LOCUS       sequence-273357-        3693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1402.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1402
FEATURES             Location/Qualifiers
     source          1..3693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..91
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     83..100
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     84..100
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     107..126
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        107..125
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(941..959)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(942..959)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(977..993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(977..993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(990..1012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1001..1017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1025..1031,1032..1049,1050..1055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1070..1091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1208..1225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1379..1967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1459..1478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2138..2929,2930..2998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2761..2780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2999..3103)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3181..3636)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3318..3339)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3530..3549
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
       61 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
      121 atagggcgaa ttgggcccga cgtcgcatgc tcccggccgc catggcggcc gcgggaattc
      181 gattggctgt tccaaagaaa accaccacca cagtgaggtg tggggcacag gtggagaagg
      241 cctttctctt gccctgagta gagggagtcc tcaggatggc agagatgatc agtatatagg
      301 agcctgcagt gaacaggcag ggactcaggc caacagtggc actggacaca tggaggacag
      361 attcattgac agaggtgtct gagcaggaga gcttcaggag caatgggaca tcacagagga
      421 agtggctgat ttggttgggg ccacacagag taagtgtgct tgtcaatact gtgtgagtca
      481 cagaattcac caagccacag agccaggatc cagtcaccaa gctgatacag acccttgtgc
      541 tcatgagaac tggataccga agagggtgac aaacggccac atagcggtca tatgccatgg
      601 cagccaacaa gacacattca gttccagcac atgacacaaa gaagaagatc tgagagaggc
      661 agccctcaaa ggagatggtc ttcttctccc tgaagaagtt cactagcatg actggaatgg
      721 aggtagatgt gtagcagatg tccaggaagc tcaagttgct gaggaaatag tacattggag
      781 tgtggagtgc agggctgacc ctggtggcca ctatcatgag tgtgttcccc aggagagttg
      841 tcaggtaaat ggccaggaag aatcactagt gaattcgcgg ccgcctgcag gtcgaccata
      901 tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata
      961 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1021 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1081 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1141 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1201 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1261 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1321 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1381 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1561 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1801 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1861 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1921 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1981 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2041 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2101 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2161 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2221 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2281 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2341 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2401 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2461 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2521 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2581 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2641 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2701 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2761 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2821 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2881 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2941 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3001 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3061 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3121 tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     3181 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3241 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3301 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3361 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3421 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3481 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3541 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3601 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
     3661 ctgttgggaa gggcgatcgg tgcgggcctc ttc
//
