LOCUS       sequence-273358-        3614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1412.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1412
FEATURES             Location/Qualifiers
     source          1..3614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..110
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     102..119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     103..119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     126..145
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        126..144
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      175..184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(771..782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(881..899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(882..899)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(917..933)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(917..933)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(930..952)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    941..957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(965..971,972..989,990..995))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1010..1031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1148..1165)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1319..1907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1399..1418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2078..2869,2870..2938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2701..2720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2939..3043)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3121..3576)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3258..3279)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3470..3489
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
       61 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
      121 aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc
      181 atggcggccg cgggaattcg attataaagc agaaggccca ggtccaagct tgggctctca
      241 ataggctagg cttagccacc tatatgctac ttatagagtc aagagatggc ttgagtcaaa
      301 gagaggaagt ttaattaata taaatacatt gttcagagta aatagcaagg tccagtgagt
      361 gacctgagtc cattagtggg ggtactgatg agaattttat gttgaaatgg gacagggatt
      421 gggacagagg acaagtgatg tggtgtggtt ggtcatgctc atcacagagg gtctccatca
      481 tagttctaga gaacagccca agaaaccctc tgctgtttga aggaaaaatt ggttctcagg
      541 aggtaattat ttcgcctcca aggagcagcc tcatccttgt agataattgt cctaatacag
      601 gggtctatcc tgagagacta tgctgctcct ggcatacaag aggatggaag aaatcacaca
      661 taaatgccag aaggaggaaa gtatgcagga agttacccag aagcaaacaa gaaaacaaga
      721 ttcaaaccac atataaactg ccctagtgat gagattgtct tccacacagc ccttccttct
      781 atctccagga tgccttcctt aatcactagt gaattcgcgg ccgcctgcag gtcgaccata
      841 tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata
      901 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      961 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1021 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1081 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1141 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1201 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2461 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3061 tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     3121 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3181 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3241 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3301 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3361 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3421 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3481 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3541 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
     3601 ctgttgggaa gggc
//
