LOCUS       sequence-273360-        1933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone plasmid with SV40 promoter and SV40 polyA with 
            Zeocin/Bleocin bacterial resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1933)
  TITLE     Piggybac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SV40-MCS-Z1
FEATURES             Location/Qualifiers
     source          1..1933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..216
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(48..68)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        66..363
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      233..368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    450..571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(461..480)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     515..534
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(730..1104)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1114..1132)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1124..1170)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(1243..1831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1323..1342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcagaagc tttactaact agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
       61 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      121 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      181 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
      241 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      301 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
      361 tggaggccta gggggtccgt acgaccggta ctcgaggagc tctataggta cctctagatg
      421 tacacatatg gctagctggc cagacatgat aagatacatt gatgagtttg gacaaaccac
      481 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      541 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      601 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      661 tatggaattc tggactcaca aagaaaaaac gcccggtgtg caagaccgag cgttctgaac
      721 aatttatcct cagtcctgct cctctgccac aaagtgcacg cagttgccgg ccgggtcgcg
      781 cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc
      841 gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg
      901 cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa
      961 cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa
     1021 cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg
     1081 aacggcactg gtcaacttgg ccatgatggc cctcctatag tgagtcgtat tatactatgc
     1141 cgatatacta tgccgatgat taattgtcaa ggatcctaag aacatgtgag caaaaggcca
     1201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1681 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1861 tgacgctcag tgaaaactca cgttaaggga ttttggtcat ggctagttaa ttaacattta
     1921 aatcagcggc cgc
//
