LOCUS       sequence-273364-        8430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM086Env
FEATURES             Location/Qualifiers
     source          1..8430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3928..3969
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3979..3996
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4019..4036)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4025..4249
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4295..4723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4382..4401)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4592..4613
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4732..4752)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4737..5066
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4917..5052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4979..4998
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5133..5927
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5187..5206)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5797..5816
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6103..6224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6140..6159)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6194..6213
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6273..6289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6273..6289)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6286..6308)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6297..6313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6321..6327,6328..6345,6346..6351))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6366..6387
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6504..6521)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6675..7263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6755..6774)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7434..8225,8226..8294))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8057..8076
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8295..8399)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcggaag caccgacgag gacctcctca gagcagtcag aatcatcaaa
     1021 atcctgtacc agagcagtaa gtaatatagg taatgacacc tttggaaatt agtgcaattg
     1081 taggactaat agtagcgcta atcttagcaa tagtagtgtg gactatagta gctatagaat
     1141 ataggaaaat actaaagcaa agaaaaatag acaggttagt taagagaata agagaaagag
     1201 cagaagacag tggaaatgag agtgaaggag acacagatga attggccaaa cttgtggaaa
     1261 tgggggactt tgatccttgg gttggtgata atctgtagtg cctcaaacaa cttgtgggtt
     1321 acagtttatt atggggttcc tgtgtggaga gatgcagata ccaccctatt ttgtgcatca
     1381 gatgccaaag cacatgagac agaagtgcac aatatctggg ccacacatgc ctgtgtaccc
     1441 acagacccca acccacaaga aatatacctg gaaaatgtaa cagaaaactt taacatgtgg
     1501 aaaaataaca tggtagagca gatgcaggag gatgtaatca gtttatggga tgaaagtcta
     1561 aagccatgtg taaagttaac tcctctctgc gttactttaa attgtacatc tgcaaatata
     1621 acaggaaatg gaatagatac aaaggtagaa aatctaacaa atgaggtaag gaactgttct
     1681 ttcaatatga ccacagaatt aagagataag aagcagaagg gctatgcact tttttataag
     1741 cttgatttag ttagtataaa taataataat actgagtata ggttaataaa ttgtaatact
     1801 tcagtcatta agcaggcttg tccaaaggca tcctttgatc caattcccat acattattgt
     1861 actccagctg gttttgcgct tttaaagtgt aatgaaaaaa tgttcaatgg gacagggcca
     1921 tgtaaaaatg tcagctcagt acaatgtacg catggaatta agccagtggt atcaactcaa
     1981 ttgctgttaa atggcagtct agcagaagac gagataataa tcagatctga aaacctcaca
     2041 gacaatgcca aaaccataat agtgcatctt aataaatctg tagaaatcaa ttgtaccaga
     2101 ccctccaaca atacaagaac aagtatgcgt ataggaccag gacaaacatt ctatagaaca
     2161 ggagaaataa caggagatat aagacaagca tattgtgaga ttaatggaac gaaatggaat
     2221 gaaactctaa aacaggtaac taaaaaatta gaaaagcact ttattaataa gacaataatc
     2281 tttcaaccac cctcaggagg agatctggaa attacaatgc accattttaa ttgtagaggg
     2341 gaatttttct attgcaatac aacaagactg tttaatggaa ccacggaggg gaataatggc
     2401 actattatac ttccatgcaa aataaaacaa attgtaagga tgtggcaggg agtaggacaa
     2461 gcaatgtatg ctcctcccat cagtggaata attaattgtg tatcaaatat tacagggcta
     2521 ctattgacaa gggatggtgg tggtaatggg actactgaga tcttcagacc tggaggagga
     2581 aatataaagg acaattggag aagtgaatta tataaatata aagtagtaca aattgaccca
     2641 ctaggagtag cacccaccag ggcaaggaga agagtggtgg agagacaaaa aagagcagtg
     2701 ggaataggag ctatgatctt tgggttctta ggagcagcag gaagcactat gggcgcggcg
     2761 tcaataacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca acagcaaagc
     2821 aatttgctga gggctataga ggcgcagcag catctgttgc aactcacagt ctggggcatt
     2881 aaacagctcc aggcaagagt cctggctgta gaaagatacc taagggatca aaagctccta
     2941 ggactttggg gctgctctgg aaaaatcatc tgcaccacta atgtgccctg gaactcctct
     3001 tggagtaata aatcttatga agagatttgg aataacatga catggacaga atgggagaga
     3061 gaaattagca attacacaaa cacaatatat ggaatactta cagaatcgca gaaccagcag
     3121 gacagaaatg aaaaggatct attggaattg gataaatgga caagtctgtg gaattggttt
     3181 gacataacaa agtggctgtg gtacataaaa atatttataa tgataatagg aggtttaata
     3241 gggttaagaa taatttttgc tgtgctttct atagtaaata gagttaggca gggatactca
     3301 cctttgtctt ttcagacccc ttcccatcat cagaggggac ccgacaggcc cgaaggaatc
     3361 gaagaagaag gtggcgagca agacagagac agatccgtga gattagtgag cggattctta
     3421 gcactctttt gggacgacct acggagcctg tgcctcttca gttaccaccg cttgagagac
     3481 ttcatcttga ttgcagtgag gattgtggaa cttctgggac gcaacagtct caagggactg
     3541 agacggggtt gggaaggact caaatatctg ggaaaccttc tgttatattg gaaacaggaa
     3601 ctaaaaatta gtgctatttc tttgcttaat gctgtagcca tagcagtagc ggggtggaca
     3661 gatagggtta tagaagcagc acaaagaact gggagggccg ttctccacat acctagaaga
     3721 atcagacagg gcttagaaag gcttttgcta taacatggga ggcaagtggt caaagggatg
     3781 gcctcaggtc agggaaagaa taaggcgaac tcctccagca gcagaaggag taggagcagt
     3841 atctcaagac ggatcaaggg tcaagacaat tctgcagata tccagcacag tggcggccgc
     3901 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     3961 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     4021 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4081 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4141 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4201 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4261 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4321 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4381 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4441 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4501 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4561 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4621 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4681 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     4741 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4801 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4861 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4921 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4981 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     5041 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     5101 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5281 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     5341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5641 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     5941 gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6001 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6061 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6241 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6841 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6901 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6961 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7021 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7081 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7141 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7201 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7261 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7321 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7381 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7441 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7501 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7561 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7621 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7681 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7741 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7801 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7861 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7921 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7981 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8041 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8101 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8161 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8221 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8281 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8341 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8401 acatttcccc gaaaagtgcc acctgacgtc
//
