LOCUS       sequence-273372-        8459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM018Env
FEATURES             Location/Qualifiers
     source          1..8459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3957..3998
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4008..4025
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4048..4065)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4054..4278
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4324..4752
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4411..4430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4621..4642
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4761..4781)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4766..5095
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4946..5081
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5008..5027
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5162..5956
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5216..5235)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5826..5845
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6132..6253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6169..6188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6223..6242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6302..6318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6302..6318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6315..6337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6326..6342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6350..6356,6357..6374,6375..6380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6395..6416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6533..6550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6704..7292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6784..6803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7463..8254,8255..8323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8086..8105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8324..8428)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcggaag caccgacgag gaactcctcg cagcagtaag aatcatcaag
     1021 atcttatacc aaagcagtaa gtatatgtaa tgtcaccttt agaaattagt gcaatagcag
     1081 gactgatagt agcattaatc ttagcaatag tagtgtggac tatagtagct atagaactta
     1141 aaaaagtact aagagagaga caaatagaaa gattaattaa gagaataaga gaaagagcag
     1201 aagacagtgg aaatgagagc gagggagaca cggatgaatt ggcaaaactt gtggaaatgg
     1261 gggactttga tccttgggtt ggggataatt tgtagtgcct cagacaactt gtgggttaca
     1321 gtttattatg gggttcctgt gtggaaagat gcagacacca ccctattttg tgcatcagat
     1381 gcccaagcac ataagacaga agtgcacaat atctgggcca cacatgcctg tgtacccaca
     1441 gaccccaacc cacaagaaat actcctagaa aatgtaacag aaaaatttaa catgtggaaa
     1501 aataacatgg tggagcagat gcaggaagat gtaatcagtt tatgggatca aagtctaaag
     1561 ccatgtgtaa agttaactcc tctctgcgtt actttacatt gtaccaatgc taatctgact
     1621 acccagtaca acgcaaccaa tgtcactaac atagtaggaa atttaacaga ggaagtaaga
     1681 aactgctctt ttaatatgac cacagaacta caagataaga agcagaaggt ccatgcactt
     1741 ttttataagc ttgatatagt acaaattgat gatgaaaata aaagtagtag tgagtataga
     1801 ctaataaact gtaatacttc agtcattaag caggcttgtc caaaagtatc ctttgatcca
     1861 attcctatac attattgtac tccagctggt tatgcgattt taaaatgtaa tgataagaag
     1921 ttcaatggga cagggccatg taaaaatgtc agctcagtac aatgcacaca tggaattaag
     1981 ccagtggtat caactcagtt gctgttaaat ggtagtctag cagaagagga gatagtaatc
     2041 agaagtgaaa atctcacaga caatgccaag accataatag tgcatcttaa taaatctgta
     2101 gacatcaatt gtaccagacc ctccaacaat acaagaacaa gtgtgtctat aggaccagga
     2161 caaatattct atagaacagg agacataata ggagatataa caaaagcata ttgtgaaatt
     2221 aatggaacaa aatggaatga aactttaaca cgggtagcta aaaaattaaa ggagcacttt
     2281 aataagacaa taatctttca accacactca ggaggagatc tagaaattac aatgcatcat
     2341 tttaattgta gaggggagtt tttctattgc aatacaacaa aactgtttaa tggtgcttgc
     2401 ccaaaaaatg gaacatgtaa tagcactatc atacttccat gtaagataaa gcgaattgta
     2461 agattgtggc aggaagtagg tcaagcaatg tatgctcctc ccatcagtgg aagcattagg
     2521 tgtaactcaa atattacagg aatactattg acaagagatg gtggtaataa tattagtaat
     2581 gaaaccttca gacctggagg aggaaatata aaggacaatt ggagaaatga attatataaa
     2641 tataaagtag tacaaattga accactagga gtagcaccca ccagggcaaa aagaagagtg
     2701 gtggagagag aaaaaagagc agtgggaata ggagctatga tctttgggtt cttaggagca
     2761 gcaggaagca ctatgggcgc ggcgtcaata acgctgacgg tacaggccag acaattattg
     2821 tctggtatag tgcaacagca aagcaatttg ctgagggcta tagaggcgca gcagcatctg
     2881 ttgcaactca cagtctgggg cattaaacag ctccaggcaa gagtcctggc tgtggaaaga
     2941 tacctaaaag atcaaaagtt cctaggactt tggggctgct ctgggaaaat catctgcacc
     3001 actgctgtgc cctggaacac cacttggagt aataaatctt atgaagagat ttggaacaac
     3061 atgacatgga tagaatggga gagagaaatt agcaattaca caaaccaaat atatgggata
     3121 cttacagaat cgcagaacca gcaggaaagg aatgaaaaag atctgttaga attggataaa
     3181 tgggcaagtc tgtggaattg gtttgacata acaagatggc tgtggtatat aaaaatgttt
     3241 ataataatag taggaagttt aataggttta agaataattt ttgctgtgct ttctatagta
     3301 aaaagagtta ggcagggata ctcacctttg tctttccaga ccccctccca tcaacagagg
     3361 gaactcggca ggcccgaagg aaccgaagaa gaaggtggag agcaaggcag aggcagatcc
     3421 gtgagattag tgaccggatt cttaccactt atctgggacg atctacggag cctgtgcctc
     3481 ttcagctacc accgcttgag agacttcatc ttgattgcaa cgaggactgt ggaacttctg
     3541 ggacagagca gtctcaaggg actgagacgg gggtgggaag gcctcaaata tctggggaat
     3601 cttctcttat attggggtca ggaactaaaa actagtgcta tttctttgtt tgatgctgca
     3661 gcaatagcag tagcggggtg gacagatagg gttatagaag tagcacaagg agcttggaga
     3721 gccattctcc acatacctag aagaatcaga cagggcttgg aaaggacttt gctataacat
     3781 gggaaacaag tggtcaaaaa catggcctcg ggtcagggaa aggttagaaa acactcctcc
     3841 agcagcagat ggagtaggag cagtatctca agacggatcc agctaagggt caagacaatt
     3901 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     3961 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
     4021 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4081 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4141 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4201 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4261 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     4321 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4381 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4441 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4501 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4561 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4621 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4681 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4741 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4801 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4861 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4921 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4981 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5041 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5101 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     5161 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     5221 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     5281 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5341 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5401 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5461 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5521 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5581 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5641 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     5701 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5761 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5821 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5881 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5941 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     6001 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6061 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6121 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6241 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6301 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6361 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6421 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6481 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6541 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6601 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6661 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6721 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6781 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6841 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6901 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6961 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7021 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7081 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7141 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7201 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7261 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7321 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7381 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7441 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7501 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7561 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7621 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7681 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7741 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7801 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7861 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7921 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7981 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8041 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8101 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8161 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8221 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8281 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
