LOCUS       sequence-273373-        8468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM067Env
FEATURES             Location/Qualifiers
     source          1..8468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3966..4007
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4017..4034
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4057..4074)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4063..4287
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4333..4761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4420..4439)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4630..4651
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4770..4790)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4775..5104
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4955..5090
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5017..5036
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5171..5965
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5225..5244)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5835..5854
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6141..6262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6178..6197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6232..6251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6311..6327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6311..6327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6324..6346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6335..6351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6359..6365,6366..6383,6384..6389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6404..6425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6542..6559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6713..7301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6793..6812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7472..8263,8264..8332))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8095..8114
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8333..8437)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcggaag caccgacgag gaactcctcg cagcagtaag aatcatcaag
     1021 atcttatacc aaagcagtaa gtatatgtaa tgacaccttt agaaattagt gcaatagcag
     1081 gactgatagt agcattaatc ttagcaatag tagtgtggac tatagtagct atagaactta
     1141 agaaagtact aagagagaga caaatagaaa aattaattaa gagaataaga gagagagcag
     1201 aagacagtgg aaatgagagc gagggagaca cggaggaatt ggcaaaactt gtggaaatgg
     1261 gggactttga tccttgggtt ggggataatt tgtagtgcct cagacaacat gtgggttaca
     1321 gtttattatg gggttcctgt gtggagagat gcagacacca ccctattttg tgcatcagat
     1381 gcccaagcac ataagacaga agtgcacaat gtctgggcca cacatgcctg tgtacccaca
     1441 gaccccaacc cacaagaaat attcctaaaa aatgtaacag aaaagtttaa catatggaaa
     1501 aataacatgg tggagcagat gcaggaagat gtaatcagtt tatgggatca aagtctaaag
     1561 ccatgtgtaa agttaactcc tctctgcgtt actttaaatt gtaccaatgc tactctgcat
     1621 gcccagaaca caaccaatgt caatgtcact aaaatagtag gaaatttaac agaggaagta
     1681 agaaactgct cttttaatat gaccacagaa ctacaagata agaagcagaa ggtccatgca
     1741 cttttttata agcttgatat agtacaaatt aatgataaaa ataatagtag tgagtatagt
     1801 gagtatagac taataaactg taatacttca gtcattaagc aggcttgtcc aaaggtatcc
     1861 tttgatccaa ttcctataca ttattgtact ccagctggtt atgcgatttt aaaatgtaat
     1921 gataagaagt ttaatgggac agggccatgt aaaaatgtca gctcagtaca atgcacacat
     1981 ggaattaagc cagtggtatc aactcagttg ctgttaaatg gtagtctagc agaagaggag
     2041 atagtaatca gaagtgaaaa tctcacagac aatgccaaga ccataatagt gcatcttaat
     2101 aaatctgtag aaatcagttg taccagaccc tccaacaata caagaacaag tgtgtctatg
     2161 ggaccaggac aagtattcta tagaacagga gacataatag gagatataac aaaagcatat
     2221 tgtgagatta atggaacaaa atggaatgaa actttaacac gggtagctaa aaaattaaag
     2281 gagcacgtta ataggacaat aatctttcga ccacccgcag gaggagatct agaaattaca
     2341 atgcatcatt ttaattgtag aggggagttt ttctattgca atacaacaaa actgtttaat
     2401 ggtacttacc cgaaaaatga aacatataat ggcactatca tacttccatg taagataaag
     2461 cgaattgtaa gattgtggca ggaagtaggt caagcaatgt atgctcctcc catcagtgga
     2521 agcattaggt gtgactcaaa tattacagga atactattga caagagatgg tgataataat
     2581 actcataatg aaaccttcag acctggagga ggaaatatga aggacaattg gagaaatgaa
     2641 ttatataaat ataaagtagt acagattgaa ccactaggag tagcacccac caaggcaaaa
     2701 agaagagtgg tggagagaga aaaaagagca gtgggaatag gagctatgat ctttgggttc
     2761 ttaggagcag caggaagcac tatgggcgcg gcgtcaataa cgctgacggt acaggccaga
     2821 caattattgt ctggtatagt gcaacagcaa agcaatttgc tgagggctat agaggcgcag
     2881 cagcatctgt tgcgactcac agtctggggc attaaacagc tccaggcaag agtcctggct
     2941 gtggaaagat acctacaaga tcaaaggttc ctaggacttt ggggctgctc tgggaaaatc
     3001 atctgcacca ctgctgtgcc ctggaacacc acttggagta ataaatctta taacgagatt
     3061 tgggacaaca tgacatggat agaatgggag agagaaatta gcaattacac aaaccaaata
     3121 tatgggatac ttacagaatc gcagaaccag caggaaagaa atgaaaagga tctgttagaa
     3181 ttggataaat gggcaagtct gtggaattgg tttgacataa caaattggct gtggtatata
     3241 agaatgttta taataatagt aggaagttta ataggtttaa gaataatttt tgctgtgctt
     3301 tctatagtaa aaagagttag gcagggatac tcacctttgt ctttccagac cccttcccat
     3361 catcagaggg aactcgacag gcccgaagga atcgaagaag aaggtggaga gcaaggcaga
     3421 ggcagatccg tgcgattagt gaccggattc ttaccactta tctgggacga tctacggagc
     3481 ctgtgcctct tcagctacca ccgcttgaga gacttcatct tgattgcaac gaggactgtg
     3541 gaacttctgg gacacagcag tctcaaggga ctgagacggg ggtgggaagg cctcaaatat
     3601 ctggggaatc ttctcttata ttggagtcag gaactaaaaa ctagtgctat ttctttgttt
     3661 gatgctgcag caatagcaat agcggggtgg acagataggg ttatagaagt agcacaagga
     3721 gcttggagag ccattctcca catacctaga agaatcagac agggcttgga aagggctttg
     3781 caataacatg ggaaacaagt ggtcaaaaac atggcctcgg gtcagggaaa gattagaaaa
     3841 cactcctcca gcagcagatg gagtaggagc agtatctcaa gacggatcca gctaagggtc
     3901 aagacaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt
     3961 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc
     4021 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     4081 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4141 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4201 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4261 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4321 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4381 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4441 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4501 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4561 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4621 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4681 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4741 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4801 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4861 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4921 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4981 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5041 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5101 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     5161 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5221 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5281 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5341 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5401 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5461 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5521 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5581 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5641 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5701 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5761 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5821 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5881 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5941 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag
     6001 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6061 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6121 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     6181 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6241 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6301 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6361 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6421 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6481 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6541 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6601 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7141 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7321 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7381 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7441 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7501 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7561 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7621 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7681 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7741 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7801 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7861 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7921 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7981 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8041 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8101 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8161 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8221 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8281 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8341 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8461 ctgacgtc
//
