LOCUS       sequence-273377-        8438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM108Env
FEATURES             Location/Qualifiers
     source          1..8438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3936..3977
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3987..4004
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4027..4044)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4033..4257
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4303..4731
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4390..4409)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4600..4621
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4740..4760)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4745..5074
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4925..5060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4987..5006
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5141..5935
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5195..5214)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5805..5824
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6111..6232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6148..6167)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6202..6221
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6281..6297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6281..6297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6294..6316)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6305..6321
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6329..6335,6336..6353,6354..6359))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6374..6395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6512..6529)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6683..7271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6763..6782)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7442..8233,8234..8302))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8065..8084
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8303..8407)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcggaag caccgacgag gaactcctca cagcagtaag aatcatcaag
     1021 atcttatacc agagcagtaa gtatatgtaa tgtcaccttt agaaattagt gcaatagcag
     1081 gactgatagt agtattaatc ttagcaatag tagtgtggac tatagtagtt atagaactta
     1141 agaaaatact aagagagaga aaaatagaaa gattagttaa gagaataaga gagagagcag
     1201 aagacagtgg aaatgagagt gagggggaca cggatgactt ggcaaaactt gtggaaatgg
     1261 gggactttga tccttgggtt ggggataatt tgtaatgcct cagacaactt gtgggttaca
     1321 gtttattatg gggttcctgt gtggagagat gcagacacca ccctattttg tgcatcagat
     1381 gcccaagcac ataagacaga agtgcacaat gtctgggcca cacatgcctg tgtacccaca
     1441 gaccccaacc cacaagaaat actcctggaa aatgtaacag aaaattttaa catgtggaaa
     1501 aataacatgg tggagcagat gcaggaagat gtaatcagtt tatgggatca aagtctaaag
     1561 ccatgtgtaa agttaactcc tctctgcgtt actttaaatt gtaccaatgc tcatctgaat
     1621 gccacaggga acaacaattt cactagcata gtaggaaatt taacagagga agtaaaaaac
     1681 tgctctttta atatgacaac agaactaaca gataagaagc agaaggtcca tgcacttttt
     1741 tataagcttg atatagtaca aattgatgat acaaataata gtagtagtga atataggcta
     1801 ataaactgta atacttcagt cattaagcag gcttgtccaa agatatcctt tgatccaatt
     1861 cctatacatt attgtactcc agctggttat gcgattttaa aatgtaatga taagaagttc
     1921 aatgggacag ggccatgtag aaatgtcagc tcagtacaat gcacacatgg aattaagcca
     1981 gtggtatcaa ctcagttgct gttaaatggt agtctagcag aagatgagat agtaatcaga
     2041 agtgaaaatc tcacagacaa tgccaagacc ataatagtgc atcttaataa atctgtagac
     2101 atcaattgta ccagaccctc caacaataca agaacaagtg tgtctatagg accaggaaga
     2161 gtattctata gaccaggaga cataatagga gatataacaa aagcatattg tgagattaat
     2221 ggaacaaaat ggaatgaaac tttaacacag gtagctaaaa aattaaagga gcactttaat
     2281 aagacaataa cctttcaacc acactcagga ggagatctag aaattacaat gcatcatttt
     2341 aattgtagag gggagttttt ctattgcaat acaacaaaac tgtttaatgg tacttgcccg
     2401 aaagatggaa catgtaatgg cactatcata cttccatgta agataaagcg aattgtaaga
     2461 ttgtggcagg aagtaggtca agcaatgtat gctcctccca tcagcggaat aattaggtgt
     2521 aactcaagta ttacaggaat actattgaca agagatggtg gtaatacgag taatgaaacc
     2581 tttagacctg gaggaggaaa tataaaggac aattggagaa gtgaattata taaatataaa
     2641 gtagtacaaa ttgaaccact aggaatagca cccaccaggg caaaaagaag agtggtggag
     2701 agagaaaaaa gagcagtggg aataggagct atgatctttg ggttcttagg agcagcagga
     2761 agcactatgg gcgcggcgtc aataacgctg acggtacagg ccagacaatt attgtctggt
     2821 atagtgcaac agcaaagcaa tttgctgaga gctatagagg cgcagcaaca tctgttgcaa
     2881 ctcacagtct ggggcattaa acagctccag gcaagagtcc tggctgtgga aagataccta
     2941 aaagatcaaa agttcctagg actttggggc tgctctggaa aaaccatctg caccactgct
     3001 gtgccctgga acaccacttg gagtaataaa tcttatgaag agatttggaa caacatgaca
     3061 tggatagaat gggagagaga aattagcaat tacacaaacc aaatatatgg gctaattaca
     3121 gaatcacaga accagcagga aaggaatgaa aaggatctgt tagaattgga taaatgggca
     3181 agtctgtgga attggtttga cataacaaat tggctgtggt atataaaaat gtttataata
     3241 atagtaggaa gcttaatagg tttaagaata atttttgctg tgctttctat agtaaataga
     3301 gttaggcagg gatactcacc tttgtctttc cagacccctt cccatcatca gagggaactc
     3361 gacaggcccg aaggaatcga agaaggaggt ggcgagcaaa acagagacag atccgtcaga
     3421 ttagtgagcg gattcttaat aatctgggac gatctacgga gcctgtgcct cttcctctac
     3481 caccgcttga gagacttcat cttgattgca acgaggactg tggaacttct gggaaggaga
     3541 ctgagacggg ggtgggaagg cctcaaatat ctggggaatc ttctattata ttggggtcag
     3601 gaactgaaaa ctagtgctat ttctttgttt gatgctgcag caatagcagt agcggggtgg
     3661 acagataggg ttatagaagt agcacaaaga gcttggagag ccattctcca catacctaga
     3721 agaatcagac agggctttga aagagctttg ctataacatg ggaaacaagt ggtcaaaaac
     3781 atggcctcgg gtcagggaaa gattagagag aactcctcca gcagcagatg gagtaggagc
     3841 agtatctcaa gacggatcca gctaagggtc aagacaattc tgcagatatc cagcacagtg
     3901 gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
     3961 tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct
     4021 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4081 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4141 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4201 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     4261 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     4321 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4381 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4441 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4501 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4561 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4621 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4681 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4741 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4801 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4861 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4921 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4981 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     5041 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5101 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     5161 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5221 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5281 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     5341 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5401 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5461 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5521 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5581 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5641 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5701 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5761 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5821 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5881 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5941 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6001 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6061 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6121 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6181 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6241 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6301 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6361 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6421 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6481 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7081 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7741 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8281 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8341 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8401 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
