LOCUS       sequence-273391-        8448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 8448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM097Env
FEATURES             Location/Qualifiers
     source          1..8448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3946..3987
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3997..4014
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4037..4054)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4043..4267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4313..4741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4400..4419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4610..4631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4750..4770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4755..5084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4935..5070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4997..5016
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5151..5945
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5205..5224)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5815..5834
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6121..6242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6158..6177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6212..6231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6291..6307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6291..6307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6304..6326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6315..6331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6339..6345,6346..6363,6364..6369))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6384..6405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6522..6539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6693..7281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6773..6792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7452..8243,8244..8312))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8075..8094
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8313..8417)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcggaaa caccgacgag gaactcctca gagcagtaag gatcatcaga
     1021 atcctgtacc aaagcagtga gtaatacaca tttgtaatga cacccttgga aattagtgca
     1081 atagtaggac tgatagtagc tctaatctta gcaatagtag tgtggactat agtatttcta
     1141 gaactcaaga aaatactaag ggaaaggaga gtagaaaggt tagttaagag aataagagaa
     1201 agagcagaag acagtggaaa tgagagtgaa ggagacacag acgaattggc caaacttgtg
     1261 gaaatggggg actttgatcc ttgggttggt gataatttgt agtgcctcag aaaatttgtg
     1321 ggttacagtt tattatgggg ttcctgtgtg gagagatgca gataccaccc tattttgtgc
     1381 atcagacgcc aaagcacagg aggcagaagc acacaatgtc tgggccacac atgcctgtgt
     1441 acccacagac cccaacccac aagaaatata cctggaaaat gtaacagaaa attttaacat
     1501 gtggaaaaat aacatggtag agcagatgca agaggatgta atcagtctat gggatcaaag
     1561 tctaaagcca tgtgtaaagt taactcctct ctgcgttact ttaaattgta gcagtgctaa
     1621 tttgaccaat accaaaaaca taaccgataa acttaacata agaaatctaa caaatgaagt
     1681 aaaaaactgt acttttaatg tgaccacaga agtaacagat aagaagcaga aggttcaggc
     1741 acttttttat acgcttgatc tagtaaaaat taataatagt agttataggt taatacattg
     1801 taatacctca gtcattaagc aggcttgtcc aaagatatcc tttgatccaa ttcctataca
     1861 ttattgtact ccggctggtt atgcgatttt aaaatgtaat gataagaatt tcaatgggac
     1921 agggccatgt aaaaatgtca gctcagtaca atgcacacat ggaattaagc cagtggtatc
     1981 aactcaatta ctgttaaatg gcagtctagc agaagataag ataataatca gatctgaaaa
     2041 tatcacagac aatgccaaaa ccataatagt gcaccttaat gaatctgtag aaatcaagtg
     2101 tatcagaccc tccaacaata caagaacaag tatatctata ggaccaggaa gagtattcta
     2161 tagaacagga gacataacag gagatataag acaagcatat tgtcagatta atgaaacaaa
     2221 atggaaaaaa gttttcaaac aggtaactga aacattaaga aggcacttta ataaaagcat
     2281 aacctttcaa gcaccaccct caggaggaga tccagaaatt acaacacatc attttaattg
     2341 tagaggggaa tttttctatt gtaatacaac aaaactgttt aattacacag caaatgaaac
     2401 tgtgtggaat gacactatca tacttccatg caggataaaa caaattgtaa acatgtggca
     2461 gggagtagga caagcaatgt atgctcctcc cgtcagggga ataattagtt gtgtatcaaa
     2521 gattacagga ttactattga caagagatgg tggtaataat aatactaacg agaccttcag
     2581 acctggggga ggaaatatga aggacaattg gagaagtgaa ttatataaat ataaagtagt
     2641 acaaattgaa ccactaggag tagcacccac cagggcaagg agaagagtgg tgcagagaga
     2701 aaagagagca gtgggaatag gagctatgat ctttgggttc ttaggagcag caggaagcac
     2761 tatgggcgca gcgtcaataa cgctgacggt acaggccaga caattgttgt ctggtatagt
     2821 gcaacagcaa agcaatttgc tgagagctat tgaggcgcag cagcatatgt tgcaactcac
     2881 agtctggggc attaaacagc tccaggcaag agtcctggct gtggaaagat acctaaaaga
     2941 tcaaaagttc ctaggacttt ggggctgctc tgggaaagtc atctgcacca ctgctgtgcc
     3001 ctggaactcc acttggagta ataaatctta taatgagatt tggaacaaca tgacatggac
     3061 agaatgggag agagaaatta gcaattacac aaaccaaata tatgatatac ttacagaatc
     3121 gcagaaccag caggacaaaa atgaaaaaga tttgttagaa cttgataaat gggcaagcct
     3181 gtggacttgg tttgatatat caaattggct gtggtatata aaaatattta taatgatagt
     3241 aggaggctta ataggtttaa gaatagtttt tgctgtaatt tccatagtaa atagagttag
     3301 gcagggatac tcacctttgt ctttccagac ccttacccgt cagcagaggg aacccgacag
     3361 gcccgaagga atcgaagaag aaggtggaga gcaaggcaga gacagatccg tgcgatttgt
     3421 gagcggattc ttagctcttg cgtgggacga tctccggagc ctgtgcctct tcagctacca
     3481 ccgcttgaga gacttcatct tgattgcagc gaggactgtg gaacttctgg gacacagcag
     3541 tctcaaggga ctgagactgg gttgggaggg cctcaaatat ctggggaacc ttctgctata
     3601 ttggagccag gaactaaaaa ctagtgctat ttctttgttt aatgctacag caataacagt
     3661 agcggggtgg acagataggg ttatagaagt agcacaaaga acttgtagag ccattctcaa
     3721 tatacctaga agaatcaggc agggcttaga aagggctttg ttataacatg ggaaacaagt
     3781 ggtcaaaaac ctggcctcaa atcagggaaa gaataaggcg aactcctcca gcagcagaag
     3841 gagtaggagc agtatctcaa gacggatcca gctaagggtc aagacaattc tgcagatatc
     3901 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     3961 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     4021 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4081 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4141 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4201 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4261 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     4321 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4381 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4441 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4501 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4561 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4621 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4681 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4741 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4801 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4861 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5041 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5101 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     5161 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5221 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5281 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5341 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5401 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5461 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5521 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5581 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5641 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5701 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5761 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5821 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5881 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5941 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca
     6001 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6061 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6121 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6181 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6241 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6301 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6361 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6421 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6481 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6541 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6601 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6661 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6721 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6781 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6841 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6901 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6961 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7021 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7081 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7141 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7201 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7261 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7321 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7381 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7441 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7501 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7561 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7621 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7681 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7741 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7801 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7861 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7921 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7981 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8041 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8101 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8161 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8221 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8281 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8401 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
