LOCUS       sequence-273394-        3486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1323.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1323
FEATURES             Location/Qualifiers
     source          1..3486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     302..321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     555..572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    689..710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(725..730,731..748,749..755)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    763..779
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     768..790
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     787..803
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     787..803
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        821..839
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     821..838
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1404..1413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1408..1417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1447..1466)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1448..1466)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1473..1490)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1473..1489)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1482..1504)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1630..2085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1717..1736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1927..1948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2163..2267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2268..2336,2337..3128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2486..2505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3299..3486,1..401)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
       61 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      481 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      541 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
      601 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      661 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      721 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      781 ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa
      841 tactcaagct atgcatccaa cgcgttggga gctctcccat atggtcgacc tgcaggcggc
      901 cgcgaattca ctagtgatta tcatccgatt tcctgcttga gatccatgcc gagagggttg
      961 caaatacgtg tacatagttg ttccatagta cagagcaact gcagtcaagt gtgacgcaca
     1021 ggtggagaag gctttctgtt tgccagaaac tgacgggatg cccatgatag tcatgaatat
     1081 agacaggtag gagacaagaa ttaccataac agaggtgaca agggtgaatc caccacagcc
     1141 aactaccaag acttgggtta cccagggatc agaacaagcc aaggccagca gtggggggat
     1201 atcacagaag aagtgtgaga tgacctgtgg ctggcagaat gacagctgtg tgatggaagt
     1261 tgttacagca accatgttaa gtaacccacc aaggtaacat cctgctacca agaggagaca
     1321 acgaactttt gacatggtaa tatggtaaag caatggccga cagatggctg tgtatcggtc
     1381 ataggccaaa tcgaattccc gcggccgcca tggcggccgg gagcatgcga cgtcgggccc
     1441 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     1501 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1561 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1621 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1681 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1741 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     1801 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     1861 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     1921 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     1981 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2041 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     2101 tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt cggggaaatg
     2161 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2221 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2281 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2341 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2401 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2461 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2521 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2581 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2641 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2701 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2761 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2821 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2881 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2941 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3001 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3061 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3121 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3181 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3241 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3421 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3481 agaact
//
