LOCUS       sequence-273404-        3995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr272.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr272
FEATURES             Location/Qualifiers
     source          1..3995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    519..540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(555..560,561..578,579..585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     598..620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     617..633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     617..633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        651..669
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     651..668
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1743..1752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1747..1756
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1786..1805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1787..1805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1812..1829)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1812..1828)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1821..1843)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1969..2424
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2056..2075)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2266..2287
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2502..2606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2607..2675,2676..3467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2825..2844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3638..3995,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga
      661 cactatagaa tactcaagct atgcatccaa cgcgttggga gctctcccat atggtcgacc
      721 tgcaggcggc cgcgaattca ctagtgattg gcgggcatcc tcctatggag atgaccaatg
      781 attccatgtt gacagaattt ctcttggtgg ggctttctga tcacccgaag ctgcagacgg
      841 ttttatttgt gctagttttg tgcatgtatc tgatgatcct gcttggaaat ggcgttctta
      901 tcgcagtagt catccatgac atccgcctgc acactcccat gtattttttc ctctgtaatc
      961 tttccttcct ggacatctgc tacacaagct cttctgtccc cctaattctc agtagcttcc
     1021 tgacagtaag gaagagagtg tccttctctg agtgtatgat tcaaatgttc ttctcctttg
     1081 ccatgggtgc cacagaatgt gtacttctag gcacgatggc acttgaccgc tttatggcca
     1141 tctgttaccc actgaggtat cctgtcatca tgagcaagga tacctatgtg cccatggcag
     1201 ctggatgctg ggttgctggg cttgttgact cagtggtaca gacctctctt gcagtgcaat
     1261 taccattctg cactaataat gtcattcacc acttcgtctg cgaaattctg ggcattctgg
     1321 aactggcttg tgcagatatt tcaatcaacg tgatcagcct aacagggtca aatctgcttt
     1381 ttctagctgt tccgttgcta gttattgctg tctcttacat gttcatcatt gcaactattt
     1441 tgaggattcc ttctgctgaa ggaaaacgga aggccttctc cacctgctct gcccacctga
     1501 cagtggtaat tatcttctat ggaaccatct tctccatgta cgcaaagccc aagtctaaag
     1561 acaccgctgg ggcaggccat caaagcgtga tggaggccct catctccctc ttctatgggg
     1621 tgatgacccc catgctcaat cctcttatct acagtctgag gaataaggat gtgaaggctg
     1681 ctgttcagaa catgctggga aggaaaacgt tgagtaagat gtaagtgaat cgaattcccg
     1741 cggccgccat ggcggccggg agcatgcgac gtcgggccca attcgcccta tagtgagtcg
     1801 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1861 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1921 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     1981 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2041 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2101 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2161 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     2221 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2281 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2341 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2401 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     2461 tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2521 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2581 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2641 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2701 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2761 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2821 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2881 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2941 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3001 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3061 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3121 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3181 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3241 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3301 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3361 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3421 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3481 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3541 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3601 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3661 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3721 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3781 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3841 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3901 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3961 ggggttcgtg cacacagccc agcttggagc gaacg
//
