LOCUS       sequence-273405-        4002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr267.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr267
FEATURES             Location/Qualifiers
     source          1..4002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     62..84
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     76..93
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     77..93
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     100..119
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        100..118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1243..1261)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1244..1261)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1279..1295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1279..1295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1292..1314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1303..1319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1327..1333,1334..1351,1352..1357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1372..1393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1510..1527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1681..2269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1761..1780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2440..3231,3232..3300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3063..3082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3301..3405)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3483..3938)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3620..3641)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3832..3851
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
       61 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
      121 gaattgggcc cgacgtcgca tgctcccggc cgccatggcg gccgcgggaa ttcgattagg
      181 aaatgccagg agaaaatgtc actgtttgga gccttttttt cctagaggga ttttcccggt
      241 acccgaggtt agagattgtt ctgtttgtct tcagccttgt catgtatctg gttaccatcc
      301 tgggtaactg tactcttatc ttaatcactg tcctggattc acgccttcaa acccccatgt
      361 acttgtttct tggaaacctc tctttcatgg atatctgtta cacgtctgct tctattccca
      421 ctttgcttgt gaacttgctg tcatctaaga aaaccattat cttttctggg tgtgctgtgc
      481 agatgtacct gtccctttcc atggggtcca cagagtgtat acttctggcg gtgatggcct
      541 atgatcgtta tgtggccatc tgtaaccctc tgaggtaccc catcatcatg aacaggcaag
      601 tctgtgtgca gatggccacc atctcctggg tgacaggctg tctgactgct ctgctggaaa
      661 ctagttttgc cctgcagatt cccctctgtg ggaacatcat caaccatttc acatgtgaaa
      721 ttcttgccgt gctaaaacta gcttgcgtga gttccctgct catggacctg gttatgctgg
      781 tggtcagtat tctccttctg cccattccaa tgctcttgat ttgcatctct tatggcttca
      841 tcctttctac aattctgagg atcagttcaa cagagggaag aaacaaagct ttttcaacct
      901 gtggtgcaca cttgactgtg gtgatcttat attatggggc tgctctctcc atgtacctga
      961 agccttcttc atcaaactca caagagatag acaaaatcat ctcgttgctt tatggagtgc
     1021 ttacccctat gttgaaccca ataatataca gcttaagaaa caaagaagta aaagatgcaa
     1081 tgataaaact gctgggcaaa gtgcccttgg caccaagtgt gtgactgtct tttattgggt
     1141 acccatgact ttcccaagga tgaatcacta gtgaattcgc ggccgcctgc aggtcgacca
     1201 tatgggagag ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa
     1261 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2101 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3421 agtgccacct gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     3481 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3541 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3601 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3661 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3721 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3781 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3841 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3901 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     3961 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat ta
//
