LOCUS       sequence-273422-        5121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5121)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-013   EB3-Eos//BsaI
FEATURES             Location/Qualifiers
     source          1..5121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2505..2626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2542..2561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2596..2615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3427..3456
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3478..4065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3966..3985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4077..4104)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4128..4919,4920..4988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4751..4770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4989..5091)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5092..5121,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tggccgtgaa cgtgtactca accagcgtaa caagcgagaa tctgtcaagg cacgacatgt
      901 tggcttgggt gaacgattcc cttcatctga actacaccaa aatcgaacag ctgtgctctg
      961 gagctgccta ttgccagttt atggacatgc tctttcccgg atgtgtgcac cttcggaaag
     1021 tcaaattcca ggcaaaactg gaacatgagt atatccacaa ctttaaggtt ctgcaagcag
     1081 cttttaagaa gatgggcgtg gacaagatca tcccggtgga aaagctcgtg aaagggaagt
     1141 ttcaggacaa tttcgagttc attcagtggt tcaagaagtt ctttgacgcc aactatgatg
     1201 ggaaagatta caatccactg cttgccagac agggacagga tgtcgcaccg ccacccaatc
     1261 ctggcgacca gatcttcaac aagagcaaga aactgattgg gacagcggtt ccacaacgta
     1321 cttcccctac tggccccaag aacatgcaga cgtctggcag actgagcaat gttgctccac
     1381 cctgtatact ccgcaagaat ccacctagtg ctcgaaatgg cggtcatgag acagatgccc
     1441 agatactgga gctcaaccaa cagctggtag acctgaaact gaccgtcgac ggattggaga
     1501 aagagaggga cttctacttc tccaagttgc gggatataga gctcatttgc caggaacacg
     1561 agagcgaaaa ctctcccgtc atcagtggta tcatcggtat tctgtatgcc actgaagaag
     1621 ggtttgcacc tcctgaagat gacgaaattg aggagcacca acaggaggat caagacgagt
     1681 acctggatgg cgggtcaggc ggcagctcag cgatcaaacc cgacatgaag atcaacctcc
     1741 gaatggaagg caacgtaaac ggccatcact tcgtgattga tggcgatggc acagggaagc
     1801 catttgaggg gaaacaaagc atggatctgg aggtgaagga aggtggtcct ctgccttttg
     1861 ccttcgacat actgactaca gcctttcact atgggaatcg cgtgtttgtc gagtatcccg
     1921 accatataca ggattacttc aagcagagtt tcccgaaagg ctatagctgg gaaaggtccc
     1981 tcacatttga ggatggaggg atttgcattg caagaaacga catcaccatg gaaggagaca
     2041 ccttctacaa caaggttcgg ttccatggga caaatttccc cgctaatggt cctgtgatgc
     2101 agaagaaaac cctgaagtgg gaaccatcca ctgagaagat gtacgtgaga gatggagtac
     2161 tgacgggtga cattcacgct gcactgctgt tggagggcaa tgcccactat cggtgtgatt
     2221 ttcgcactac ctacaaggcc aaagagaaag gcgtcaaact ccctggatac caccttgtcg
     2281 atcattgcat cgagatcctt tctcacgaca aggactacaa caaggtgaag ctgtatgaac
     2341 atgccgttgc tcactctgga ttgccagaca atgcaaggcg ttcaggctct gattaaggcg
     2401 ccaggtacat ctccaatacg gcgtcttgca tagagaacac gattagcaca ccggttaccc
     2461 acgtgatgcc cgaacggacc catgtcatgc cagagcgcac tggtaacttg tttattgcag
     2521 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2581 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtcgtc
     2641 gaattcgagt tggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc
     2701 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     2761 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     2821 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     2881 gacgtaatta catccctggg ggctttggga gggggctgtc cccgtgagct cccaggcgcg
     2941 cctgtcattc taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc
     3001 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
     3061 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
     3121 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
     3181 gggggctttg ggggggggct gtccccgtga gctcccaggc gcgccaactc ggtacctcac
     3241 ccgattctca tcatggagac acatcaggac atctccaata ccgccatgga aacgcaccaa
     3301 gatgcgactg ctcttctgcc agtgacggca ttcgtctgca agattaacgg catacagaaa
     3361 gaaacgatga gacccattca ggacgagcct cagactccag cgtaactgga ctgcaatcaa
     3421 ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag gatcttcttg
     3481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3541 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
     3601 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3661 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3721 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3781 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3841 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3901 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3961 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4021 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgca
     4081 gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatta ccaatgctta
     4141 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4201 cccgtcgtgt agataactac gatgcgggag ggcttaccat ctggccccag tgctgcaatg
     4261 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccggg
     4321 agggccgagc gcagaagtga tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4381 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4441 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4501 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4561 ggtcctccga tcgttgccag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4621 gcactgcata attctcttac tgtcatgcca tccgtgagat gcttttctgt gactggtgag
     4681 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4741 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4801 cgttcttcgg ggcgtaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4861 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4921 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4981 atactcattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     5041 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aagtcaaaag
     5101 cctccggtcg gaggcttttg a
//
