LOCUS       sequence-27343-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Liu H, Nishitoh H, Ichijo H, Kyriakis JM
  TITLE     Activation of apoptosis signal-regulating kinase 1 (ASK1) by tumor 
            necrosis factor receptor-associated factor 2 requires prior 
            dissociation of the ASK1 inhibitor thioredoxin.
  JOURNAL   Mol Cell Biol. 2000 Mar . 20(6):2198-208.
  PUBMED    10688666
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Mar . 20(6):2198-208."
COMMENT     Sequence Label: FLAG-TRX
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(633..651)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(634..651)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(671..688)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccaccgc catggactac aaggacgatg
      121 atgacaaggg cgaattcatg gtgaagcaga tcgagagcaa gactgctttt caggaagcct
      181 tggacgctgc aggtgataaa cttgtagtag ttgacttctc agccacgtgg tgtgggcctt
      241 gcaaaatgat caagcctttc tttcattccc tctctgaaaa gtattccaac gtgatattcc
      301 ttgaagtaga tgtggatgac tgtcaggatg ttgcttcaga gtgtgaagtc aaatgcatgc
      361 caacattcca gttttttaag aagggacaaa aggtgggtga attttctgga gccaataagg
      421 aaaagcttga agccaccatt aatgaattag tctaatcatg ttttctgaaa acataaccag
      481 ccattggcta tttaaaactt gtaatttttt taatttacaa aaatataaaa tatgaagaca
      541 taaacccagt tgccatctgc gtgacaataa aacattaatg ctaacaaaaa aaaaaaaaaa
      601 aaaaactcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
      661 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      721 gtgccttcct tga
//
