LOCUS       sequence-273431-       15565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host range bacterial artificial chromosome vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15565)
  AUTHORS   Nasrin S, Ganji S, Kakirde KS, Jacob MR, Wang M, Ravu RR, Cobine PA,
            Khan IA, Wu CC, Mead DA, Li XC, Liles MR
  TITLE     Chloramphenicol Derivatives with Antibacterial Activity Identified 
            by Functional Metagenomics.
  JOURNAL   J Nat Prod. 2018 Jun 22;81(6):1321-1332. doi: 
            10.1021/acs.jnatprod.7b00903. Epub 2018 Jun 13.
  PUBMED    29897754
REFERENCE   2  (bases 1 to 15565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jnatprod.7b00903"; journalName: "J Nat Prod"; date: 
            "2018-06-22- 22"; volume: "81"; issue: "6"; pages: "1321-1332"
COMMENT     Sequence Label: pSmartBAC-S
FEATURES             Location/Qualifiers
     source          1..15565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(254..265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(831..2648)
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     protein_bind    2658..2757
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             2828..2839
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     oriT            4280..4389
                     /note="incP origin of transfer"
     CDS             complement(4615..5586)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(5586..6761)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    7087..7337
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(7340..8095)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      8186..8405
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      8473..9004
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8481..9095
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     terminator      complement(9862..9889)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        9948..9966
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9948..9965
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(10024..10308)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHATSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDAA"
     primer_bind     10029..10048
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10242..10264
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10256..10273
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10257..10273
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10304..10320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10304..10320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10317..10339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10328..10344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10352..10358,10359..10376,10377..10382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(10535..10552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10706..11294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10786..10805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11401..12735,12736..12822))
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(12823..13268)
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     primer_bind     complement(13344..13363)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13345..13363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      13386..13431
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        13435..13537
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             13538..14197
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(13604..13623)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(14211..14242)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    14268..14301
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    14319..14717
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
ORIGIN
        1 ccccccccct tccggggaca cccccctatc cgtcgacacc cccctaccta ctcacgtgag
       61 taggctgggc gcggcagcgc tagaagcgac cgcccgtcat gaagtgggca gtgagccaac
      121 ccatgaaggc cagtagcgca aagcgtcgag cacgcaccca acccgaaggc ttgcctgtac
      181 tgccgctttg cgtggcgaac catgaccaga cgtgttcgct cagcgtgtcg cccggcttct
      241 tattgaagag cgctcgaccc tcaatgacgc cgaacgcggc aagccatgcg agccaagcga
      301 ttgtgtatcc gctcattgag ccccccgacg gattcgaacc gccgaccccc tgattacgaa
      361 acaggcgctc taaccgaact gagctaggga ggcaacgccg gtcgctcaac cctgggagag
      421 atcaggggag tgaccggcct gagtgggtag cgctcgacgc cgttccttag cggtgcttga
      481 tgcgtggcgc gtaaacccct ctacctatat gaaggttgtc ggcaacctga ttcggggtca
      541 cagcgaaggg cgcccgggtg acgctgtgat gaagtggcgt gaagtctgga ttacccaatg
      601 agaagtcatt aggaatgtgt gtttcgagtc attccgtcac agcatcactt cccccaggtc
      661 aacggctcgc cccgctgtgc tgtggtgacg gagtcactgt tacttaggct aactaactaa
      721 accgagacaa gagaaatggg ccgaccgggg gaccggccga cccttcccgc tgccccagga
      781 agcccgctgt gcgcccgtct cagcgcctaa caggcttccc gggtgtctcg ctacgccgct
      841 acgtcttccg tgccgtcctg ggcgtcgtct tcgtcgtcgt cggtcggcgg cttcgcccac
      901 gtgatcgaag cgcgcttctc gatgggcgtt ccctgccccc tgcccgtagt cgacttcgtg
      961 acaacgatct tgtctacgaa gagcccgacg aacacgcgct tgtcgtctac tgacgcgcgc
     1021 ccccaccacg acttagggcc ggtcgggtca gcgtcggcgt cttcggggaa ccattggtca
     1081 aggggaagct tcggggcttc ggcggcttca agttcggcaa gccgctcttc cgccccttgc
     1141 tgccggagcg tcagcgctgc ctgttgcttc cggaagtgct tcctgccaac gggtccgtcg
     1201 tacgcgcctg ccgcgcggtc ttcgtacagc tcttcaaggg cgttcagggc gtcggcgcgc
     1261 tccgcaacaa ggttcgcccg ttcgccgctc ttctcaggcg cctcagtgag cttgccgaag
     1321 cgtcgggcgg cttcccacag aagcgccaac gtctcttcgt cgccttcggc gtgcctgatc
     1381 ttgttgaaga tgcgttccgc aacgaacttg tcgagtgccg ccatgctgac gttgcacgtg
     1441 ccttcgtgct gcccaggtgc ggacgggtcg accaccttcc ggcgacggca gcggtaagag
     1501 tccttgatcg attcttcccc gcgcttcgaa gtcatgacgg cgccacactc gcagtacagc
     1561 ttgtccatgg cggacagaat ggcttgcccc cgggaaagcc ccttgccgcg ccccctgccg
     1621 tccaaccacg cctgaagctc ataccactca gcgggctcga tgatcggtcc gcaatcaagc
     1681 tcgaccggcc ggagcgtgat cgggtcgcgc tgaatgcggt aaccctcaat cttcgtggtc
     1741 ggcgtgccgt ccggcttctt cttgtagatc acctcagcgg cgaagcccgc aatacgcggg
     1801 tcccgaagga ttcgcataac ggttgccggg tcccaggcgc ttgaagcggt cttcttccca
     1861 atcgtctcgc cccgggtcgg cacggcgtca gcgtccatgc gcttacaaag ccccgtgatg
     1921 ctgcccgggt gaatggcggc ttgactgccc ggcttgaagg gaaggtgttt gtgcgtcttg
     1981 atctcacgcc accaccaccg gattacgtcg ggctcgaact cgaagggtcc ggtaagggga
     2041 gtggtcgagt gcgcaagctt gttgatgacg acattgacca ttcggccgtt gcgcgtgatc
     2101 tccttcgtct ccgaaacaag ctcgaagccg taaggcgcct tcccgccgac gtacccgccc
     2161 aattcgcgct gaaggttctt cgtgtcgaga atcttcgccg acttcagcga agattctttg
     2221 tgcgacgcgt cgagccgcat aatcaggtga atcaggtcca tgacgtttcc ctgccggaag
     2281 acgccttcct gagtggaaac aatcgtcacg cccagggcga gcaattccga gacaatcgga
     2341 atcgcgtcca tgaccttcag gcgcgagaag cgcgacacgt catagacaat gatcatgttg
     2401 agccgcccgg cgcggcattc gttcaggatg cgttcgaact ccgggcgctc cgccgtcccg
     2461 aacgccgacg tgcccggcgc ttcgctgaaa tgcccgacga acctgaaccg gcccccgtcg
     2521 cgctcgactt cgcgctgaag gtcggccgcc ttgtcttcgt tggcgctacg ctgtgtcgct
     2581 gggcttgctg cgctcgaatt ctcgcgctcg cgcgactgac ggtcgtaagc acccgcgtac
     2641 gtgtccaccc cggtcacaac cccttgtgtc atgtcggcga ccctacgccc ccaactgaga
     2701 gaactcaaag gttaccccag ttggggcact actcccgaaa accgcttctg acctgggaaa
     2761 acgtgaagcc ccggggcatc cgctgagggt tgccgccggg gcttcggtgt gtccgtcagt
     2821 acgggccata gaggggcgtc gtgtaaccca cccagggcga ttgctccggc acggggaagc
     2881 cgcgccacgc cttcgggacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccg
     2941 gacgagcgtc tgctccgcca ttcgccgtcc gccgtgccaa tcggatcagc cgtccaaatg
     3001 cgggattttc gttagtcgga ggccaaacgg cattgagcgt cagcatatca tcagcgagct
     3061 gaagaaagac aatccccgat ccgctccacg tgttgcccca gcaatcagcg cgaccttgcc
     3121 cctccaacgt catctcgttc tccgctcatg agctcagcca ctggcgagcg gcatcgcatt
     3181 cttcgcatcc cgcctctggc ggatgcagga agatcaacgg atctcggccc agttgaccca
     3241 gggctgtcgc cacaatgtcg cgggagcgga tcaaccgagc aaaggcatga ccgactggac
     3301 cttccttctg aaggctcttc tccttgagcc acctgtccgc caaggcaaag cgctcacagc
     3361 agtggtcatt ctcgagataa tcgacgcgta ccaacttgcc atcctgaaga atggtgcagt
     3421 gtctcggcac cccataggga acctttgcca tcaactcggc aagatgcagc gtcgtgttgg
     3481 catcgtgtcc cacgccgagg agaagtacct gcccatcgag ttcatggaca cgggcgaccg
     3541 ggcttgcagg cgagtgaggt ggcaggggca atggatcaga gatgatctgc tctgcctgtg
     3601 gccccgctgc cgcaaaggca aatggatggg cgctgcgctt tacatttggc aggcgccaga
     3661 atgtgtcaga gacaactcca aggtccggtg taacgggcga cgtggcagga tcgaacggct
     3721 cgtcgtccag acctgaccac gagggcatga cgagcgtccc tcccggaccc agcgcagcac
     3781 gcagggcctc gatcagtcca agtggcccat cttcgagggg ccggacgcta cggaaggagc
     3841 tgtggaccag cagcacaccg ccgggggtaa ccccaaggtt gagaagctga ccgatgagct
     3901 cggcttttcg ccattcgtat tgcacgacat tgcactccac cgctgatgac atcagtcgat
     3961 catagcacga tcaacggcac tgttgcaaat agtcggtggt gataaactta tcatcccctt
     4021 ttgctgatgg agctgcacat gaacccattc aaaggccggc attttcagcg tgacatcatt
     4081 ctgtgggccg tacgctggta ctgcaaatac ggcatcagtt accgtgagct gcaggtcgac
     4141 ggatcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     4201 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     4261 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     4321 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     4381 gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc
     4441 caaccaggaa gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga
     4501 ttgaggaaaa ggcggcggcg gccgagagag aatattcagg ccagttatgc tttctggcct
     4561 gtaacaaagg acattaagta aagacagata aacgtagact aaaacgtggt cgcatcaggg
     4621 tgctggcttt tcaagttcct taagaatggc ctcaattttc tctatacact cagttggaac
     4681 acgagacctg tccaggttaa gcaccatttt atcgccctta tacaatactg tcgctccagg
     4741 agcaaactga tgtcgtgagc ttaaactagt tcttgatgca gatgacgttt taagcacaga
     4801 agttaaaaga gtgataactt cttcagcttc aaatatcacc ccagcttttt tctgctcatg
     4861 aaggttagat gcctgctgct taagtaattc ctctttatct gtaaaggctt tttgaagtgc
     4921 atcacctgac cgggcagata gttcaccggg gtgagaaaaa agagcaacaa ctgatttagg
     4981 caatttggcg gtgttgatac agcgggtaat aatcttacgt gaaatatttt ccgcatcagc
     5041 cagcgcagaa atatttccag caaattcatt ctgcaatcgg cttgcataac gctgaccacg
     5101 ttcataagca cttgttgggc gataatcgtt acccaatctg gataatgcag ccatctgctc
     5161 atcatccagc tcgccaacca gaacacgata atcactttcg gtaagtgcag cagctttacg
     5221 acggcgactc ccatcggcaa tttctatgac accagatact cttcgaccga acgccggtgt
     5281 ctgttgacca gtcagtagaa aagaagggat gagatcatcc agtgcgtcct cagtaagcag
     5341 ctcctggtca cgttcattac ctgaccatac ccgagaggtc ttctcaacac tatcaccccg
     5401 gagcacttca agagtaaact tcacatcccg accacataca ggcaaagtaa tggcattacc
     5461 gcgagccatt actcctacgc gcgcaattaa cgaatccacc atcggggcag ctggtgtcga
     5521 taacgaagta tcttcaaccg gttgagtatt gagcgtatgt tttggaataa caggcgcacg
     5581 cttcattatc taatctccca gcgtggttta atcagacgat cgaaaatttc attgcagaca
     5641 ggttcccaaa tagaaagagc atttctccag gcaccagttg aagagcgttg atcaatggcc
     5701 tgttcaaaaa cagttctcat ccggatctga cctttaccaa cttcatccgt ttcacgtaca
     5761 acatttttta gaaccatgct tccccaggca tcccgaattt gctcctccat ccacggggac
     5821 tgagagccat tactattgct gtatttggta agcaaaatac gtacatcagg ctcgaaccct
     5881 ttaagatcaa cgttcttgag cagatcacga agcatatcga aaaactgcag tgcggaggtg
     5941 tagtcaaaca actcagcagg cgtgggaaca atcagcacat cagcagcaca tacgacatta
     6001 atcgtgccga tacccaggtt aggcgcgctg tcaataacta tgacatcata gtcatgagca
     6061 acagtttcaa tggccagtcg gagcatcagg tgtggatcgg tgggcagttt accttcatca
     6121 aatttgccca ttaactcagt ttcaatacgg tgcagagcca gacaggaagg aataatgtca
     6181 agccccggcc agcaagtggg ctttattgca taagtgacat cgtccttttc cccaagatag
     6241 aaaggcagga gagtgtcttc tgcatgaata tgaagatctg gtacccatcc gtgatacatt
     6301 gaggctgttc cctgggggtc gttaccttcc acgagcaaaa cacgtagccc cttcagagcc
     6361 agatcctgag caagatgaac agaaactgag gttttgtaaa cgcctccttt atgggcagca
     6421 accccgatca ccggtggaaa tacgtcttca gcacgtcgca atcgcgtacc aaacacatca
     6481 cgcatatgat taatttgttc aattgtataa ccaacacgtt gctcaacccg tcctcgaatt
     6541 tccatatccg ggtgcggtag tcgccctgct ttctcggcat ctctgatagc ctgagaagaa
     6601 accccaacta aatccgctgc ttcacctatt ctccagcgcc gggttatttt cctcgcttcc
     6661 gggctgtcat cattaaactg tgcaatggcg atagccttcg tcatttcatg accagcgttt
     6721 atgcactggt taagtgtttc catgagtttc attctgaaca tcctttaatc attgctttgc
     6781 gtttttttat taaatcttgc aatttactgc aaagcaacaa caaaatcgca aagtcatcaa
     6841 aaaaccgcaa agttgtttaa aataagagca acactacaaa aggagataag aagagcacat
     6901 acctcagtca cttattatca ctagcgctcg ccgcagccgt gtaaccgagc atagcgagcg
     6961 aactggcgag gaagcaaaga agaactgttc tgtcagatag ctcttacgct cagcgcaaga
     7021 agaaatatcc accgtgggaa aaactccagg tagaggtaca cacgcggata gccaattcag
     7081 agtaataaac tgtgataatc aaccctcatc aatgatgacg aactaacccc cgatatcaag
     7141 tcacatgacg aagggaaaga gaaggaaatc aactgtgaca aactgccctc aaatttggct
     7201 tccttaaaaa ttacagttca aaaagtatga gaaaatccat gcaggctgaa ggaaacagca
     7261 aaactgtgac aaattaccct cagtaggtca gaacaaatgt gacgaaccac cctcaaatct
     7321 gtgacagata accctcagac tatcctgtcg tcatggaagt gatatcgcgg aaggaaaata
     7381 cgatatgagt cgtctggcgg cctttctttt tctcaatgta tgagaggcgc attggagttc
     7441 tgctgttgat ctcattaaca cagacctgca ggaagcggcg gcggaagtca ggcatacgct
     7501 ggtaactttg aggcagctgg taacgctcta tgatccagtc gattttcaga gagacgatgc
     7561 ctgagccatc cggcttacga tactgacaca gggattcgta taaacgcatg gcatacggat
     7621 tggtgatttc ttttgtttca ctaagccgaa actgcgtaaa ccggttctgt aacccgataa
     7681 agaagggaat gagatatggg ttgatatgta cactgtaaag ccctctggat ggactgtgcg
     7741 cacgtttgat aaaccaagga aaagattcat agcctttttc atcgccggca tcctcttcag
     7801 ggcgataaaa aaccacttcc ttccccgcga aactcttcaa tgcctgccgt atatccttac
     7861 tggcttccgc agaggtcaat ccgaatattt cagcatattt agcaacatgg atctcgcaga
     7921 taccgtcatg ttcctgtagg gtgccatcag attttctgat ctggtcaacg aacagataca
     7981 gcatacgttt ttgatcccgg gagagactat atgccgcctc agtgaggtcg tttgactgga
     8041 cgattcgcgg gctattttta cgtttcttgt gattgataac cgctgtttcc gccatgacag
     8101 atccatgtga agtgtgacaa gtttttagat tgtcacacta aataaaaaag agtcaataag
     8161 cagggataac tttgtgaaaa aacagcttct tctgagggca atttgtcaca gggttaaggg
     8221 caatttgtca cagacaggac tgtcatttga gggtgatttg tcacactgaa agggcaattt
     8281 gtcacaacac cttctctaga accagcatgg ataaaggcct acaaggcgct ctaaaaaaga
     8341 agatctaaaa actataaaaa aaataattat aaaaatatcc ccgtggataa gtggataacc
     8401 ccaagggaag ttttttcagg catcgtgtgt aagcagaata tataagtgct gttccctggt
     8461 gcttcctcgc tcactcgacc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc
     8521 gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa
     8581 aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc
     8641 tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag
     8701 cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca
     8761 agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg
     8821 cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa
     8881 atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa
     8941 cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc
     9001 ggccggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca
     9061 tagacggccg ccagcccagc ggcgagggca accagccgag ggcttcgccc tgtcgctcga
     9121 ctgcggcgag cactactggc tgtaaaagga cagaccacat catggttctg tgttcattag
     9181 gttgttctgt ccattgctga cataatccgc tccacttcaa cgtaacaccg cacgaagatt
     9241 tctattgttc ctgaaggcat attcaaatcg ttttcgttac cgcttgcagg catcatgaca
     9301 gaacactact tcctataaac gctacacagg ctcctgagat taataatgcg gatctctacg
     9361 ataatgggag attttcccga ctgtttcgtt cgcttctcag tggataacag ccagcttctc
     9421 tgtttaacag acaaaaacag catatccact cagttccaca tttccatata aaggccaagg
     9481 catttattct caggataatt gtttcagcat cgcaaccgca tcagactccg gcatcgcaaa
     9541 ctgcacccgg tgccgggcag ccacatccag cgcaaaaacc ttcgtgtaga cttccgttga
     9601 actgatggac ttatgtccca tcaggctttg cagaactttc agcggtatac cggcatacag
     9661 catgtgcatc gcataggaat ggcggaacgt atgtggtgtg accggaacag agaacgtcac
     9721 accgtcagca gcagcggcgg caaccgcctc cccaatccag gtcctgaccg ttctgtccgt
     9781 cacttcccag atccgcgctt tctctgtcct tcctgtgcga cggttacgcc gctccatgag
     9841 cttatcgggt ccactcgcga acgcagaaag gcccacccga aggtgagcca gtgtgattac
     9901 atttgcggcc taactgtggc cagtccagtt acgctggagt cactagtatt taggtgacac
     9961 tatagaagcg gccgcgacaa cttgtccacg tggaattcta agcttaggat cccaatgtga
    10021 tggctatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    10081 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10141 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10201 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10261 aacgacggcc agtgaagtcg catgcaaact tggcgtaatc atggtcatag ctgtttcctg
    10321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    10381 aagcctgggg tgcctaatga gtgagctaac ccacattaat tgcgttgcgc tcactgcccg
    10441 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    10501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10981 ccgaccgctg cgccttatcc agtaactatc gtcttgagtc caacccggta agacacgact
    11041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    11101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    11161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    11221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    11281 aaaaaggatc tcaagaagat cctttgatct tttctaccag cacagacgtc ggattcacct
    11341 ttatgttgat aagaaataaa agaaaatgcc aataggatat cggcattttc ttttgcgttt
    11401 ttatttgtta actgttaatt gtccttgttc aaggatgctg tctttgacaa cagatgtttt
    11461 cttgcctttg atgttcagca ggaaacttgg cgcaaacgtt gattgtttgt ctgcgtagaa
    11521 tcctctgttt gtcatatagc ttgtaatcac gacattgttt cctttcgctt gaggtacagc
    11581 gaagtgtgag taagtaaagg ttacatcgtt aggatcaaga tccattttta acacaaggcc
    11641 agttttgttc agcggcttgt atgggccagt taaagaatta gaaacataac caagcatgta
    11701 aatatcgtta gacgtaatgc cgtcaatcgt catttttgat ccgcgggagt cagtgaacag
    11761 gtaccatttg ccgttcattt taaagacgtt cgcgcgttca atttcatctg ttactgtgtt
    11821 agatgcaatc agcggtttca tcactttttt cagtgtgtaa tcatcgttta gctcaatcat
    11881 accgagagcg ccgtttgcta actcagccgt gcgtttttta tcgctttgca gaagtttttg
    11941 actttcttga cggaagaatg atgtgctttt gccatagtat gctttgttaa ataaagattc
    12001 ttcgccttgg tagccatctt cagttccagt gtttgcttca aatactaagt atttgtggcc
    12061 tttatcttct acgtagtgag gatctctcag cgtatggttg tcgcctgagc tgtagttgcc
    12121 ttcatcgatg aactgctgta cattttgata cgtttttccg tcaccgtcaa agattgattt
    12181 ataatcctct acaccgttga tgttcaaaga gctgtctgat gctgatacgt taacttgtgc
    12241 agttgtcagt gtttgtttgc cgtaatgttt accggagaaa tcagtgtaga ataaacggat
    12301 ttttccgtca gatgtaaatg tggctgaacc tgaccattct tgtgtttggt cttttaggat
    12361 agaatcattt gcatcgaatt tgtcgctgtc tttaaagacg cggccagcgt ttttccagct
    12421 gtcaatagaa gtttcgccga ctttttgata gaacatgtaa atcgatgtgt catccgcatt
    12481 tttaggatct ccggctaatg caaagacgat gtggtagccg tgatagtttg cgacagtgcc
    12541 gtcagcgttt tgtaatggcc agctgtccca aacgtccagg ccttttgcag aagagatatt
    12601 tttaattgtg gacgaatcga actcaggaac ttgatatttt tcattttttt gctgttcagg
    12661 gatttgcagc atatcatggc gtgtaatatg ggaaatgccg tatgtttcct tatatggctt
    12721 ttggttcgtt tctttcgcaa acgcttgagt tgcgcctcct gccagcagtg cggtagtaaa
    12781 ggttaatact gttgcttgtt ttgcaaactt tttgatgttc atcgttcatg tctccttttt
    12841 tatgtactgt gttagcggtc tgcttcttcc agccctcctg tttgaagatg gcaagttagt
    12901 tacgcacaat aaaaaaagac ctaaaatatg taaggggtga cgccaaagta tacactttgc
    12961 cctttacaca ttttaggtct tgcctgcttt atcagtaaca aacccgcgcg atttactttt
    13021 cgacctcatt ctattagact ctcgtttgga ttgcaactgg tctattttcc tcttttgttt
    13081 gatagaaaat cataaaagga tttgcagact acgggcctaa agaactaaaa aatctatctg
    13141 tttcttttca ttctctgtat tttttatagt ttctgttgca tgggcataaa gttgcctttt
    13201 taatcacaat tcagaaaata tcataatatc tcatttcact aaataatagt gaacggcagg
    13261 tatatgtgat gggttaaaat gcatccacca cagtgggatc cgaagcttgg aattcacgtg
    13321 acttgaagtc gcggccgcac tgaccctata gtgagtcgta ttaatttaaa tcataccaac
    13381 atggtcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt aatctgatcg
    13441 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
    13501 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
    13561 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
    13621 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
    13681 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
    13741 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
    13801 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
    13861 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
    13921 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
    13981 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
    14041 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
    14101 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
    14161 acaacagtac tgcgatgagt ggcagggcgg ggcgtaacct aggtgacaga agttaaaagc
    14221 ctccggtcgg aggcttttga ctttctgcta gatcggccgc atcgaatata acttcgtata
    14281 atgtatgcta tacgaagtta ttagcgatga gctcggactt ccattgttca ttccacggac
    14341 aaaaacagag aaaggaaacg acagaggcca aaaagctcgc tttcagcacc tgtcgtttcc
    14401 tttcttttca gagggtattt taaataaaaa cattaagtta tgacgaagaa gaacggaaac
    14461 gccttaaacc ggaaaatttt cataaatagc gaaaacccgc gaggtcgccg ccccgtaacc
    14521 tgtcggatca ccggaaagga cccgtaaagt gataatgatt atcatctaca tatcacaacg
    14581 tgcgtggagg ccatcaaacc acgtcaaata atcaattatg acgcaggtat cgtattaatt
    14641 gatctgcatc aacttaacgt aaaagcaact tcagacaata caaatcagcg acactgaata
    14701 cggggcaacc tcatgtccga gctcgcgagc tcgtcgacag cgacacactt gcatcggatg
    14761 cagcccggtt aacgtgccgg cacggcctgg gtaaccaggt attttgtcca cataaccgtg
    14821 cgcaaaatgt tgtggataag caggacacag cagcaatcca cagcaggcat acaaccgcac
    14881 accgaggtta ctccgttcta caggttacga cgaatgtcaa tacttgccct tgacaggcat
    14941 tgatggaatc gtagtctcac gctgatagtc tgatcgacaa tacaagtggg accgtggtcc
    15001 cagaccgata atcagaccga caacacgagt gggatcgtgg tcccagacta ataatcagac
    15061 cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga
    15121 ccgtggtycc agwctratwa tcagaccgac gatacgagtg ggaccgtggt cccagactaa
    15181 taatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag accgacgata
    15241 cgagtgggac cgtggtccca gtctgattat cagaccgacg atacaagtgg aacagttatg
    15301 cccgggaatt cctgcaggat cgatccgctc gagaagcttt ctagtggtac caacccaggc
    15361 aggtagcaca gtgcgcgccc actgccttac ggatagcacg acgcatcttc gccgcagcgt
    15421 tgttcccacg ggcgatagca gcacgccgct tcgcatggct cttcacactg cgctgtgcct
    15481 gataggtggc gtggtgctga gcacagcgcc caccatgagt agcccagtcc ctacagtcga
    15541 gacaccgggt acgcataggg caccc
//
