LOCUS       sequence-273436-        9613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ABE and ST2-com modified sgRNA scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Lyu P, Lu Z, Cho SI, Yadav M, Yoo KW, Atala A, Kim JS, Lu B
  TITLE     Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like 
            Particles Show High On-Target Base Editing and Undetectable RNA 
            Off-Target Activities.
  JOURNAL   CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1089/crispr.2020.0095.
  PUBMED    33616436
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2020"; journalName: "CRISPR J"; date: "2021-02"; 
            volume: "4"; issue: "1"; pages: "69-81"
COMMENT     Sequence Label: pspCas9-ABE-3'UTR-sgRNA-ST2-com vector
FEATURES             Location/Qualifiers
     source          1..9613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        144..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     675..694
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        675..693
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             743..763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1952..1975,1976..1978,1979..6052)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1952..6052
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6053..6100
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6410..6427)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6416..6623
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6635..6775
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6635..6764
                     /label=AAV2 ITR
     rep_origin      6850..7305
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6937..6956)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7147..7168
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7322..7341)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7441..7463
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7501..7519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7587..7691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7692..7760,7761..8552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7910..7929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8723..9311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9212..9231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(9373..9613,1..8)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9373..9393
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9544..9563
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gaaacaccgg tgtcttcctc gaggaagacc cgtttgagag ctagaaatag caagttcaaa
       61 taaggctagt ccgttatcaa cttggctgaa tgcctgcgag catcccaccc aagtggcacc
      121 gagtcggtgc tttttttggt acccgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      661 agatccgcgg ccgctaatac gactcactat agggagagcc gccaccatga aacggacagc
      721 cgacggaagc gagttcgagt caccaaagaa gaagcggaaa gtctctgaag tcgagtttag
      781 ccacgagtat tggatgaggc acgcactgac cctggcaaag cgagcatggg atgaaagaga
      841 agtccccgtg ggcgccgtgc tggtgcacaa caatagagtg atcggagagg gatggaacag
      901 gccaatcggc cgccacgacc ctaccgcaca cgcagagatc atggcactga ggcagggagg
      961 cctggtcatg cagaattacc gcctgatcga tgccaccctg tatgtgacac tggagccatg
     1021 cgtgatgtgc gcaggagcaa tgatccacag caggatcgga agagtggtgt tcggagcacg
     1081 ggacgccaag accggcgcag caggctccct gatggatgtg ctgcaccacc ccggcatgaa
     1141 ccaccgggtg gagatcacag agggaatcct ggcagacgag tgcgccgccc tgctgagcga
     1201 tttctttaga atgcggagac aggagatcaa ggcccagaag aaggcacaga gctccaccga
     1261 ctctggagga tctagcggag gatcctctgg aagcgagaca ccaggcacaa gcgagtccgc
     1321 cacaccagag agctccggcg gctcctccgg aggatcctct gaggtggagt tttcccacga
     1381 gtactggatg agacatgccc tgaccctggc caagagggca cgcgatgaga gggaggtgcc
     1441 tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc gagggctgga acagagccat
     1501 cggcctgcac gacccaacag cccatgccga aattatggcc ctgagacagg gcggcctggt
     1561 catgcagaac tacagactga ttgacgccac cctgtacgtg acattcgagc cttgcgtgat
     1621 gtgcgccggc gccatgatcc actctaggat cggccgcgtg gtgtttggcg tgaggaacgc
     1681 aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac taccccggca tgaatcaccg
     1741 cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc gccctgctgt gctatttctt
     1801 tcggatgcct agacaggtgt tcaatgctca gaagaaggcc cagagctcca ccgactccgg
     1861 aggatctagc ggaggctcct ctggctctga gacacctggc acaagcgaga gcgcaacacc
     1921 tgaaagcagc gggggcagca gcggggggtc agacaagaag tacagcatcg gcctggccat
     1981 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
     2041 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
     2101 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
     2161 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
     2221 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
     2281 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
     2341 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
     2401 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
     2461 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
     2521 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
     2581 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
     2641 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
     2701 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
     2761 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
     2821 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
     2881 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
     2941 cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca aagagatttt
     3001 cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca gccaggaaga
     3061 gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg aactgctcgt
     3121 gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc
     3181 ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag atttttaccc
     3241 attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca tcccctacta
     3301 cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa agagcgagga
     3361 aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg cccagagctt
     3421 catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc tgcccaagca
     3481 cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga aatacgtgac
     3541 cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca tcgtggacct
     3601 gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact acttcaagaa
     3661 aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     3721 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     3781 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     3841 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     3901 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     3961 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     4021 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     4081 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     4141 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     4201 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     4261 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     4321 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     4381 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     4441 ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga aggacgactc
     4501 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     4561 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     4621 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     4681 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     4741 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     4801 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     4861 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     4921 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     4981 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     5041 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     5101 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     5161 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     5221 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     5281 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     5341 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     5401 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     5461 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     5521 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     5581 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     5641 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     5701 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     5761 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     5821 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     5881 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     5941 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     6001 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg acaaaaggcc
     6061 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctaag ctcgctttct
     6121 tgctgtccaa tttctattaa aggttccttt gttccctaag tccaactact aaactggggg
     6181 atattatgaa gggccttgag catctggatt ctgcctaata aaaaacattt attttcattg
     6241 ctagctcgct ttcttgctgt ccaatttcta ttaaaggttc ctttgttccc taagtccaac
     6301 tactaaactg ggggatatta tgaagggcct tgagcatctg gattctgcct aataaaaaac
     6361 atttattttc attgcgaatt ctagaaaatt cctagagctc gctgatcagc ctcgactgtg
     6421 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6481 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6541 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6601 gagaatagca ggcatgctgg ggaggtacgg ccgcaggaac ccctagtgat ggagttggcc
     6661 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     6721 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     6781 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     6841 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6901 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     6961 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7021 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     7081 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7141 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     7201 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     7261 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     7321 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     7381 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     7441 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     7501 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7561 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7621 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7921 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8221 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8401 agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9361 ttgctcacat gtgagggcct atttcccatg attccttcat atttgcatat acgatacaag
     9421 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     9481 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     9541 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     9601 ttgtggaaag gac
//
