LOCUS       sequence-273442-        3418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1507
FEATURES             Location/Qualifiers
     source          1..3418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      762..771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      766..775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(805..824)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(806..824)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(831..848)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(831..847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(840..862)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      988..1443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1075..1094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1285..1306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1521..1625
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1626..1694,1695..2486)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1844..1863)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2657..3245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3146..3165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3399..3416
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      241 caagctattt aggtgacact atagaatact caagctatgc atccaacgcg ttgggagctc
      301 tcccatatgg tcgacctgca ggcggccgcg aattcactag tgatttctga ttctctcagt
      361 cccttcaaag atacatgggt attctgtctg gtagtttgaa tacaacgaaa attatcttcc
      421 agtaaaagaa acaactttct tcaagttggg tagtcagaac atctcgtgtt actgtagtcc
      481 tggagagcct gcttgtcgtg tcagggcaga gaggcactat tccagattcc tgagcaaacc
      541 aaccctttgc atctatactt tttgcccttg tctcactatg caaataactc tgcagcaaga
      601 atttctagga tggtgttcag cagactgaag ttgtgcgaca aggacagtgt atcttctgtg
      661 tcttttcaga cccagcgatg aggagggatt ccacgctgtg agcaaccagt ttcagagctg
      721 taccagtttg tcaggacaat ttaaaaaatc gaattcccgc ggccgccatg gcggccggga
      781 gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
      841 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      901 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      961 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     1021 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1081 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1141 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1201 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1261 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1321 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1381 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1441 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatcaggtg
     1501 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1561 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1621 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1681 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1741 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1801 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1861 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1921 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1981 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2041 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2101 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2161 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2221 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2281 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2341 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2401 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2461 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2521 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2581 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2641 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2701 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2761 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2821 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2881 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2941 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3001 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3061 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3121 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3181 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3241 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3301 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3361 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgagga
//
