LOCUS       sequence-273451-       10162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Three input / three output logic circuit plasmid - Expresses 
            different intein split ECFs from different inducible promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Pinto F, Thornton EL, Wang B
  TITLE     An expanded library of orthogonal split inteins enables modular 
            multi-peptide assemblies.
  JOURNAL   Nat Commun. 2020 Mar 23;11(1):1529. doi: 10.1038/s41467-020-15272-2.
  PUBMED    32251274
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-23"; volume: "11(1):1529. doi"; pages: " 
            10.1038/s41467-020-15272-2"
COMMENT     Sequence Label: pFP.E222
FEATURES             Location/Qualifiers
     source          1..10162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..69
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(73..116)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    119..140
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(141..1019)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1046..1330
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     terminator      2565..2636
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2652..2679
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(2757..3593)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        complement(3611..3645)
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      3661..3688
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3746..3864
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     RBS             3876..3887
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        4622..4740
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     terminator      5222..5293
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5309..5336
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        5351..5385
                     /label=BBa_J23115
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.15"
     RBS             5397..5408
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      6212..6283
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6299..6326
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             7008..7019
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    7631..7651
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(7791..8606)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7791..8606)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8514..8533
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8598..8617
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(9201..9745)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9280..9299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
      121 attcgcggcc gcttctagag ttatgacaac ttgacggcta catcattcac tttttcttca
      181 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa
      241 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg
      301 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc
      361 cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg
      421 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg
      481 cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt
      541 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg
      601 gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga
      661 aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc
      721 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga
      781 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc
      841 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc
      901 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc
      961 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata
     1021 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
     1081 cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa
     1141 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
     1201 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
     1261 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
     1321 gtttctccat tactagagtc acacaggact actagatgaa tgaaaccgat cctgatctgg
     1381 aactgctgaa acgtattggt aataatgatg cacaggccgt taaagaaatg gttacccgta
     1441 aactgcctcg tctgctggca ctggcaagtc gcctgctggg tgatgcagat gaagcacgtg
     1501 atattgcaca agaaagtttt ctgcgcattt ggaaacaggc agcaagctgg cgtagcgaac
     1561 aggcacgttt tgatacctgg ctgcatcgtg ttgcactgaa tctgtgttat gatcgtctgc
     1621 gtcgtcgtaa agaacatgtg ccggttgata gcgaacatgc ctgtgaagtg gatgcttgca
     1681 tctcgggaga tagtttgatc agcttggcga gcacagggaa aagagttcct attaaggatt
     1741 tgttaggcga aaaagatttt gaaatatggg caattaatga acagacgatg aagctggaat
     1801 cagctaaagt tagtcgtgta ttttgtaccg gcaaaaagct agtctatact ctaaaaactc
     1861 gactaggtag aactatcaag gcaacagcaa atcatagatt tttaactatt gatggttgga
     1921 aaagattaga tgagctatct ttaaaagagc atattgctct accccgtaaa ctagaataat
     1981 aatactagag ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt
     2041 cgttttggtc ctactagagg ctatgccata gcatttttat ccataagatt agcggatcct
     2101 acctgacgct ttttatcgca actctctact gtttctccat tactagagtc acacaggaaa
     2161 gtactagatg gaagcagtat taaattacaa tcacagaatt gtaaatattg aagctgtgtc
     2221 agaaacaatc gatgtttatg atattgaggt tccccacacc cacaattttg ctttggcaag
     2281 cggagtgttt gtccataaca gtgcaaaatt tgcagcaagt gatgaacagc ctgcacaggc
     2341 aagccgtgat ctgggtaaac tgctgggtca gctgccggat cgtcagcgtc tgccgattgt
     2401 tcatgttaaa ctggaaggtc tgagcgttga agaaaccgca cagattaccg gtctgagcag
     2461 cagcgcagtt aaagttggta ttcatcgtgg tctgaaagca ctgggcaaac tgattcgtgg
     2521 taaaggtcat gatgaagatc gttaataata ctagagccag gcatcaaata aaacgaaagg
     2581 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2641 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagggaccaa
     2701 aacgaaaaaa gacgcttttc agcgtcttat tgttcgtctt tggtaccgag ctagtattat
     2761 tgcagaaagc catcccgtcc ctggcgaata tcacgcggtg accagttaaa ctctcggcga
     2821 aaaagcgtcg aaaagtggtt actgtcgctg aatccacagc gataggcgat gtcagtaacg
     2881 ctggcctcgc tgtggcgtag cagatgtcgg gctttcatca gtcgcaggcg gttcaggtat
     2941 cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgtacg cagtgaaaga
     3001 gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag
     3061 gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa actgctttta
     3121 cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga gggtaaatca
     3181 ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg caatacgctg
     3241 tggttaacgc gccagtgaga cggatactgc ccatccagct cttgtggcag caactgattc
     3301 agcccggcga gaaactgaaa tcgatccggc gagcgataca gcacattggt cagacacaga
     3361 ttatcggtat gttcatacag atgccgatca tgatcgcgta cgaaacagac cgtgccaccg
     3421 gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc gacaatcaca
     3481 atttcatgaa aatcatgatg atgttcagga aaatccgcct gcgggagccg gggttctatc
     3541 gccacggacg cgttaccgga cggaaaaaaa tccacactat gtaatacggt catttgggct
     3601 ccctctagta gctagcataa tacctaggac tgagctagct gtaaactcta gtatcacact
     3661 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagaccttt acgccgctgg
     3721 agcaggaatg cggtgagcat cacatcacca caattcagca aattgtgaac atcatcacgt
     3781 tcatctttcc ctggttgcca atggcccatt ttcctgtcag taacgagaag gtcgcgaatt
     3841 gaggcgcttt ttagactggt cgtatactag agattaaaga ggagaaatac tagatggcac
     3901 gtgttagcgg tgcagcagca gccgaagcag cactgatgcg tgcactgtat gatgaacatg
     3961 cagcagttct gtggcgttat gcactgcgtc tgaccggtga tgcagcacag gcagaagatg
     4021 ttgttcaaga aaccctgctg cgtgcatggc agcatccgga agttattggt gataccgcac
     4081 gtccggcacg cgcatggctg tttaccgttg cacgtaatat gattattgat gaacgtcgta
     4141 gcgcacgttt tcgtaatgtt gttggtagca ccgataatcc gtgttgtctg gttggtagca
     4201 gcgaaatcat tacccgtaat tatggtaaaa ccaccatcaa agaagtggtc gagatcttcg
     4261 ataacgacaa aaacattcag gtgctggcct ttaataccca taccgataat attgaatggg
     4321 caccgattaa agcagcacag ctgacccgtc cgaatgcaga actggttgaa ctggaaattg
     4381 ataccctgca tggtgttaaa accattcgtt gtacaccgga tcatcctgtg tataccaaaa
     4441 atcgtggtta tgttcgtgca gatgaactga ccgatgatga tgaactggtg gttgcaattt
     4501 aataatacta gagaggaaac acagaaaaaa gcccgcacct gacagtgcgg gctttttttt
     4561 tcgaccaaag gtactagagg acctttacgc cgctggagca ggaatgcggt gagcatcaca
     4621 tcaccacaat tcagcaaatt gtgaacatca tcacgttcat ctttccctgg ttgccaatgg
     4681 cccattttcc tgtcagtaac gagaaggtcg cgaattgagg cgctttttag actggtcgta
     4741 tactagagtc acacaggaaa gtactagatg agctcctctt tacaattggc accagaaata
     4801 gaaaagttgc ctcagagtga tatttactgg gaccccatcg tttctattac ggagactgga
     4861 gtcgaagagg tttttgattt gactgtgcca ggactacgta actttgtcgc caatgacatc
     4921 attgtacata acagcgatct gacccgtccg gcaccggatg aacagctgga agcaagcgca
     4981 cagagccgtc gtatggcaca ggcactggat cagctgccgg atcgtcagcg tgaagcaatt
     5041 gttctgcagt attatcaaga actgagcaat accgaagcag cagcactgat gcaaattagc
     5101 gttgaagccc tggaaagcct gctgagccgt gcacgtcgta atctgcgtag ccatctggcc
     5161 gaagcaccgg gtgcagatct gagcggtcgt cgcaaaccgt aataatacta gagccaggca
     5221 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     5281 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     5341 tatactagag tttatagcta gctcagccct tggtacaatg ctagctacta gagattaaag
     5401 aggagaaata ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa
     5461 ttaaagcttg tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg
     5521 tacattgtga atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata
     5581 tttcaatcct agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa
     5641 taaaatatga tcctatagta gattattcta actccaatca ttcaccaatt aattggaata
     5701 tatttgaaaa caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat
     5761 caggtcttat cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc
     5821 ttagttttgc acattcagaa aaagacaact atatagatag tttattttta catgcgtgta
     5881 tgaacatacc attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa
     5941 ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg
     6001 aaggaaaaag ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt
     6061 tccatttaac caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta
     6121 aagcaatttt aacaggagca attgattgcc catactttaa aaattaataa cactgatagt
     6181 gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     6241 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     6301 ctcaccttcg ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt
     6361 ttacgcaaga aaatggtttg ttactttcga ataaatacta gagtcacaca ggactactag
     6421 atgcagcgta ccaatagcca ggatgttctg agcacccgtg aaagccagct gcaggcatta
     6481 ctgctgcagg gtctggcagg cgataccttt gcatatcgtc agtttctgac cgcactggca
     6541 gcacatattc gtggttttct gcgtcgtcgt ctgccgcagc atccggcaga agttgaagat
     6601 ctgctgcaag aagttctgct ggcagttcat aatgcacgtc atacctatca ggcacgtcag
     6661 ccgctgaccg catgggttca ggcaattgca cgttataaac tggcagatca tctgcgtagc
     6721 catgcacgtc gtgaagcacg tcatgatctg ctggcaggtg gttgtttttc tggagataca
     6781 ttagtcgctt taactgatgg tcgtagcgtt agctttgagc aattggttga agaagaaaaa
     6841 caaggaaaac aaaacttttg ttataccatc cgccatgatg gttctatagg ggttgaaaaa
     6901 atcatcaatg cccgcaaaac aaaaactaat gcgaaggtaa tcaaggttac gttggacaat
     6961 ggtgagtcta ttatttgcac cccggatcat aaattcatgt tgcgggatgg gagctacaaa
     7021 tgtgcgatgg atttaactct cgatgattcg ttaatgccgt tacaccgaaa aatttcgact
     7081 acggaagatt ctggtcatgc gtaataatac tagagctcgg taccaaattc cagaaaagag
     7141 gcctcccgaa aggggggcct tttttcgttt tggtcctact agagacctgt aggatcgtac
     7201 aggtttacgc aagaaaatgg tttgttactt tcgaataaat actagagtca cacaggacta
     7261 ctagatggaa gccaaaacct atatcggcaa actgaaaagc cgtaaaattg tgagcaacga
     7321 ggatacctat gatattcaga ccagcaccca taactttttc gccaatgata ttctggtgca
     7381 taattcagag atccagagca caccggatga agttaatgca gcactggatc gtctgctgat
     7441 tgcagatgca ctggcacagc tgagcgcaga acatcgtgca gttattcagc gtagctatta
     7501 tcgtggttgg agcaccgcac agattgcaac cgatctgggt attgcagaag gcaccgttaa
     7561 aagccgtctg cattatgcag ttcgtgccct gcgcctgacc ctgcaagaac tgggtgttac
     7621 ccgttaataa tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc
     7681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatct cgagtcccgt
     7741 caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac
     7801 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcgat accatatttt
     7861 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     7921 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     7981 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     8041 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc
     8101 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     8161 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     8221 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     8281 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     8341 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     8401 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     8461 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     8521 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctggagcaa
     8581 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     8641 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     8701 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg
     8761 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg
     8821 tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg
     8881 gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcagcgctag
     8941 cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg
     9001 tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc
     9061 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     9121 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     9181 agggccgcgg caaagccgtt tttccatagg ctccgccccc tgacaagcat cacgaaatct
     9241 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9301 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     9361 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     9421 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     9481 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
     9541 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
     9601 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
     9661 tcagagaacc ttcaaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
     9721 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aaggggtctg acgctcagtg
     9781 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9841 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9901 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9961 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10021 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10081 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10141 ctccatccag tctattccat gg
//
