LOCUS       sequence-273454-        4010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr155.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr155
FEATURES             Location/Qualifiers
     source          1..4010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     44..66
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     58..75
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     59..75
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     82..101
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        82..100
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      131..140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1233..1251)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1234..1251)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1269..1285)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1269..1285)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1282..1304)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1293..1309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1317..1323,1324..1341,1342..1347))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1362..1383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1500..1517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1671..2259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1751..1770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2430..3221,3222..3290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3053..3072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3291..3395)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3473..3928)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3610..3631)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3822..3841
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
       61 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg cccgacgtcg
      121 catgctcccg gccgccatgg cggccgcggg aattcgattt caaaccgaat gtaccatgga
      181 cagatccaat gagaccgccc ccctgtccgg cttcattctc ctgggcctct ctgcccaccc
      241 aaagctggag aaaaccttct tcgtgctcat cctgatgatg tacctggtga tcctgctggg
      301 caacggcgtc ctcatcctgg tgagcatcct cgactcccac ctgcacacgc ccatgtactt
      361 cttcctgggg aacctctcct tcctggacat ctgctacact acctcctctg tccccctcat
      421 tctggacagc tttctgactc ccaggaagac catctccttc tcgggctgtg ccgtgcagat
      481 gtttctctcc ttcgccatgg gagccacgga gtgtgtgctc ctgagtatga tggcgtttga
      541 tcgttatgtg gccatctgca acccccttag atatcctgtg gtcatgaaca aggctgccta
      601 tgtgcccatg gctgccagtt cctgggcagg tggtatcact aattctgtag tgcagacatc
      661 tttggcaatg cggctgccct tctgtgggga caatgtcatc aatcacttca cctgtgagat
      721 cctggcagtc ctgaaactgg cctgtgctga catctccatc aatgtcatca gcatggttgt
      781 ggccaacatg atcttcttgg cagtcccagt cctcttcatc tttgtctcct atgtcttcat
      841 ccttgtgaca atcctgagga tcccctctgc tgaggggagg aagaaggcct tctccacctg
      901 ctctgcccac ctcaccgtgg tacttgtctt ctatggaacc atcctcttca tgtacgggaa
      961 gcccaagtcc aaggacccac tgggggcaga caagcaggac cttgcagaca agctcatctc
     1021 cctcttctat ggagtggtga cccccatgct aaaccccatc atctacagct tgagaaacaa
     1081 ggacgtgagg gctgctgtga ggaacctggt gggccagaaa cacctaactg agtgctgtca
     1141 cagtgcagaa ctaatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag
     1201 ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg
     1261 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1321 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1381 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1441 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2101 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3421 gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     3481 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3541 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3601 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3661 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3721 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3781 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3841 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3901 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
     3961 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
//
