LOCUS       sequence-273455-        3754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr157.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr157
FEATURES             Location/Qualifiers
     source          1..3754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     365..382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    499..520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(535..540,541..558,559..565)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    573..589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     578..600
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     597..613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     597..613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        631..649
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     631..648
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1482..1491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1486..1495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1525..1544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1526..1544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1551..1568)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1551..1567)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1560..1582)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1708..2163
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1795..1814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2005..2026
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2241..2345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2346..2414,2415..3206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2564..2583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3377..3754,1..211)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
       61 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      361 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      601 aaacagctat gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct
      661 atgcatccaa cgcgttggga gctctcccat atggtcgacc tgcaggcggc cgcgaattca
      721 ctagtgattg caagtcacag atgcctcacc ttctaatgga gggatagagc ccatgatcca
      781 catgttctga tggctctcac ttgagaatct tattgcaagg caatgctagg tgaggaacaa
      841 gctgtcttag agtcattcct agtgtgatcc acagactgga aatgtcaaca tcacccatca
      901 cccgggagct ttttggcata cagagtttcc tgacccccag cagatctact gactgggaaa
      961 gttcatttta acaagatcct ccactggatt tatatgcatg ctggacctgg agaagtatca
     1021 gttaagacca tggaatggat gccctggtgt attgtctgga tccccagaaa tacttatcac
     1081 cccagccact ggacctgtca gtgactgcca acatctggat ctctctgcag cactgctcct
     1141 tactaaggag agccacctca cccagtgcca cactctcttc ttacatcagc cctcatcctg
     1201 tcaccatcat tggtaatgag aaggctttgt tatctctaaa agcttcgcca caagtgcttc
     1261 tctgtggcag ttcatgttgt cccctgctcg ggcctgatcc ctttaacagt tgccacagca
     1321 aacatcctgc atgcaaatct ctccacaaga gtcagcttca ggggaaaccg gcctttgaca
     1381 caaaacaacc acgtcagcac aggtgtgtga accatgtgtg tgttaccagt gtgtgccctt
     1441 tgcagccaat gcttgtgctg gcttccaatc gaattcccgc ggccgccatg gcggccggga
     1501 gcatgcgacg tcgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     1561 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     1621 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1681 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     1741 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1801 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1861 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1921 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1981 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2041 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2101 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     2161 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatcaggtg
     2221 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2281 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2341 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2401 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2461 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2521 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2581 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2641 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2701 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2761 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2821 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2881 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2941 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3001 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3061 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3721 gcttggagcg aacgacctac accgaactga gata
//
