LOCUS       sequence-273456-        3999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr273.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr273
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     235..254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     488..505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    622..643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(658..663,664..681,682..688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    696..712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     701..723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     720..736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     720..736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        754..772
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     754..771
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1850..1859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1854..1863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1893..1912)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1894..1912)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1919..1936)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1919..1935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1928..1950)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2076..2531
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2163..2182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2373..2394
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2609..2713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2714..2782,2783..3574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2932..2951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3745..3999,1..334)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
       61 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      421 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      481 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      541 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      601 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      661 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      721 aggaaacagc tatgaccatg attacgccaa gctatttagg tgacactata gaatactcaa
      781 gctatgcatc caacgcgttg ggagctctcc catatggtcg acctgcaggc ggccgcgaat
      841 tcactagtga ttctgatgtc tctaaggaac gtacttttgt ttcagtatgt attttacggc
      901 agctttaaca tctttattcc tcaagctgta gatgattgga ttcagcatgg gggtcactac
      961 tccgtaaaat aaagaaatga tcttatctga ggtttggaac ttgtcttgcc cagtcagatc
     1021 ttgagatttg ggttttgcat acatggagaa gatagttcca taaaatatga tcaccacagt
     1081 caggtgggca gagcacgtgg agaatgcctt tcgtctccct gtggctgagt tcattctcaa
     1141 gattgtgtgg aggatgaaca ggtaggagaa aaagatgact agtagtggaa gaaccaggaa
     1201 ggccatattt gatatgacca tggtaatgat attgagagat atatcagcac atgctagctt
     1261 gaggacagct aagacctcac acgtaaaatg attaatcaca ttattcccac agaaaggcag
     1321 ccgcatggca agggatgttt gcacaactga attgatgctg ccagagaacc atgatgcgga
     1381 tgccatgaac acatacactt ctttgctcat gatgatggag tatctcagag ggttgcagat
     1441 ggccacgtag cgatcaaaag ccatcatgcc aagtagcaaa cattctgttg atcccattgc
     1501 aaatccaaag aacatctgca ctgcacaacc agagaaagaa atgtttcttt tctttgaaat
     1561 taggctcacc aatgttgaag ggacagaaga tgttgtgtag cagatatcca ggaaagagag
     1621 gttgcccagg aagaagtaca tgggtgtgtg aagacgggaa tcaaagatgc ttgctatgat
     1681 cagaacacca ttgccagtta gaatcactag atacatgacc agaacaataa caaagtaaat
     1741 gatctcagtc tttgggtatc cagcaagacc caaaagaagg aattcagaaa caaacgtctg
     1801 gttattttta tccatgtcat ccacggtcag ccatcatcga attcccgcgg ccgccatggc
     1861 ggccgggagc atgcgacgtc gggcccaatt cgccctatag tgagtcgtat tacaattcac
     1921 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1981 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2041 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
     2101 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     2161 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2221 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2281 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2341 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2401 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2461 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2521 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2581 atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2641 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2701 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     2761 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     2821 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     2881 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     2941 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3001 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3061 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3121 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3181 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3241 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3301 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3361 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3421 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3481 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3541 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3601 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3661 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3901 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3961 gctaatcctg ttaccagtgg ctgctgccag tggcgataa
//
