LOCUS       sequence-273463-        3931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr49.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr49
FEATURES             Location/Qualifiers
     source          1..3931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..85
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     77..94
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     78..94
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     101..120
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        101..119
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1173..1191)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1174..1191)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1209..1225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1209..1225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1222..1244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1233..1249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1257..1263,1264..1281,1282..1287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1302..1323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1440..1457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1611..2199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1691..1710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2370..3161,3162..3230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2993..3012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3231..3335)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3413..3868)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3550..3571)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3762..3781
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
       61 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg
      121 cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgattcg
      181 tctggctgaa cttttggaac ttgcttacgt gttccctaaa tacctgcttc acctgcttgt
      241 tcctcagagt gtagatgaag gggttgagtg tgggtgtcac tacagtgttg agcaatgcca
      301 ccaccttgtt atgatcctcc ccctgtccat tcttgccaga ccggacatac atgaagatac
      361 agctgccata gaagagagac accacaataa tatgagagga gcaagttgag aaggctttct
      421 tcctctcctt ggctgaagga atgtgtagaa tggtatagag aatgtggcca tagcaggtgg
      481 cagtcacagc cagagtgccc aggaggctga aattggcaat aacaaaaccc aggaactcta
      541 ccaggctggt atctgcacat atgagttcca taagtggaaa gttgtcacag aagaaatgat
      601 taatgatgtt tgggccacag aacggctgct gaaatacaat tgaactagga actatgatga
      661 gaagcaatcc agacatccat gagcaaaaca caagctggac acagactctt ttgctcataa
      721 tggtggcata acgcaaaggg ttacaaatgg ccacatacct gtcaaaggac atcactgcca
      781 gtagaaagaa ctcagtggtg ccaaggaaga aatagaggaa tgcttggagg aaacaaccta
      841 gtagggagat ggtcttatgt cctgtgatga tgttggttag catcttgggg aagatgacag
      901 aggtgaacca gatctccagc atggcaaagt tgcggaggaa gtaatacatg ggggtgtaaa
      961 ggcgcctgtc cacaagggtg atgaagatga tgaggaagtt tcccagcaga atgaggaagt
     1021 aggtcaggag aaagcccagg aatatgagca cctgcagctc acaggcatct gacagcccca
     1081 gcagaataaa ctaatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag
     1141 ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg
     1201 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1261 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1321 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1381 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1441 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1501 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1561 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1621 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1681 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1741 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1801 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1861 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1921 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1981 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2041 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2101 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2161 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2221 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2281 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2341 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2401 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2461 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2521 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2581 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2641 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2701 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2761 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2821 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2881 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2941 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3001 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3061 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3121 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3181 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3241 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3301 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3361 gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     3421 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3481 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3541 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3601 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3661 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3721 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3781 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3841 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
     3901 aagggcgatc ggtgcgggcc tcttcgctat t
//
