LOCUS       sequence-273464-        3228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr71.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr71
FEATURES             Location/Qualifiers
     source          1..3228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     81..98
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    215..236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(251..256,257..274,275..281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    289..305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     294..316
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     313..329
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     313..329
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        347..365
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     347..364
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      672..681
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      676..685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(715..734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(716..734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(741..758)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(741..757)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(750..772)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      898..1353
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(985..1004)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1195..1216
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1431..1535
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1536..1604,1605..2396)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1754..1773)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2567..3155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3056..3075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
       61 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      121 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      181 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      241 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      301 aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt aggtgacact
      361 atagaatact caagctatgc atccaacgcg ttgggagctc tcccatatgg tcgacctgca
      421 ggcggccgcg aattcactag tgattctaac ttgtcttgag gtcagcccag agactactgt
      481 ggaggcccag ggacaggtgc taggcagcag cctggaccag gacagggcag gagcagaaag
      541 tgacttgtga gtgattctgt gtcaggatta gtgggatttg gtactgtgtc tctggagtgt
      601 aggtctccat agctccatgt tctgtcccac agtggtattg gcataggctg atccacaatc
      661 gaattcccgc ggccgccatg gcggccggga gcatgcgacg tcgggcccaa ttcgccctat
      721 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      781 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      841 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
      901 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      961 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1021 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1081 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1141 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1201 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     1261 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     1321 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     1381 tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1441 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1501 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1561 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1621 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1681 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1741 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1801 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1861 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1921 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1981 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2041 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2101 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2161 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2221 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2281 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2341 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2401 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2461 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2521 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2581 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2641 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2701 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2761 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2821 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2881 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2941 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3001 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3061 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgt
//
