LOCUS       sequence-273469-        3667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr6
FEATURES             Location/Qualifiers
     source          1..3667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     431..448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    565..586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(601..606,607..624,625..631)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    639..655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     644..666
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     663..679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     663..679
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        697..715
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     697..714
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1461..1470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1465..1474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1504..1523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1505..1523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1530..1547)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1530..1546)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1539..1561)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1687..2142
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1774..1793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1984..2005
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2220..2324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2325..2393,2394..3185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2543..2562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3356..3667,1..277)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
       61 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      301 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      361 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      421 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      481 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      541 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      601 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      661 cacaggaaac agctatgacc atgattacgc caagctattt aggtgacact atagaatact
      721 caagctatgc atccaacgcg ttgggagctc tcccatatgg tcgacctgca ggcggccgcg
      781 aattcactag tgattgctgg attcctattt cgccccaata ccatttcttt cttgggatgt
      841 atgactcagc tttacttctt tatgtccctt gcctgtactg aatgtgtact tttagctgcc
      901 atggcctatg atcgttatgt agccatatgt tggcctcttc gctatccagt catgatgacc
      961 acaggatttt gtgttcaact gaccatcagt tcttgggtga gtggcttcac catctccatg
     1021 gcaaaggtgt actttctctc acgagttgcc ttctgtggca ataatgtctt gaaccatttt
     1081 ttctgtgatg tgtcgcccat ccttaagtta gcttgtatga acttatctat ggctgagaca
     1141 gtagattttg cccttgccat cgtcatcctt atattcccac tctcagccac tgttctttcc
     1201 tatggcttca ttgtctctac tgtcctgcaa ataccatcag ccactgggca gcggaaggcc
     1261 ttctccacct gtgcttctca tcttacagtg gtggtcatct tctacacagc tgtgatcttc
     1321 atgtatgtcc gacctcgggc cattgcttcg tttaattcta acaagctgat ctcagccatc
     1381 tatgcagtct tcactcccat gctcaacccc attatctact gcctgaggaa caaggaagtc
     1441 aaagaaatcg aattcccgcg gccgccatgg cggccgggag catgcgacgt cgggcccaat
     1501 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     1561 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     1621 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1681 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1741 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1801 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1861 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1921 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1981 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     2041 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2101 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     2161 ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc
     2221 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2281 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2341 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2401 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2461 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2521 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2581 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2641 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2701 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2761 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2821 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2881 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2941 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3001 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3061 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3121 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3181 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3241 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3301 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3361 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3421 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3481 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3541 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3601 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3661 agcggtc
//
