LOCUS       sequence-273482-        5709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5709)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-019   Vimentin-mVenus//BsaI
FEATURES             Location/Qualifiers
     source          1..5709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     primer_bind     complement(2559..2578)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    3093..3214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3130..3149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3184..3203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      4015..4044
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4066..4653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4554..4573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4665..4692)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4716..5507,5508..5576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5339..5358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5577..5679)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5680..5709,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgagcaccag gagtgtcagt agctcctcct ataggcgtat gtttggcgga cctggaacag
      901 cttctcgacc cagttcatct cggtcatacg tcacgacttc aacacgaacc tatagcctgg
      961 gaagtgcgct taggcctagc actagtcgca gcttgtacgc atcttctcca ggtggggtgt
     1021 atgccacgcg ttctagtgcc gtgagactgc gttcttctgt tcctggcgta agactcctgc
     1081 aggattcagt cgacttcagc cttgcagacg ccatcaacac cgagttcaag aataccagga
     1141 ctaacgagaa ggtggagctg caggagctga atgatcggtt cgctaactac atcgataaag
     1201 tgagatttct cgagcagcag aacaagattc tgctggcaga gctggaacag ctgaaagggc
     1261 aaggcaaatc cagactcgga gacttgtacg aggaggaaat gcgggagctc aggcgacaag
     1321 tggatcagct gacaaacgac aaagctcgcg tggaagtcga gagagacaat ctggccgaag
     1381 atatcatgcg gcttcgggag aagttgcagg aagaaatgct gcagcgagag gaggcggaga
     1441 acacactcca gtcctttcgc caggatgtgg acaatgcttc cctggcacgg ctggacctcg
     1501 agaggaaagt tgaaagcctc caggaagaga tagcctttct caagaaactg cacgaagagg
     1561 aaatccagga actgcaagct cagattcagg agcaacacgt gcagattgat gtggacgtgt
     1621 ctaagcccga ccttaccgct gcactccgcg atgtccggca gcagtacgag tcagttgccg
     1681 ctaagaactt gcaagaagca gaggaatggt acaagagcaa gttcgctgac ctgagcgaag
     1741 cagcgaatcg caacaatgac gctcttagac aagccaagca ggagtctacg gagtatcgca
     1801 gacaggttca atccctgact tgcgaagttg atgccctgaa ggggaccaat gagagcttgg
     1861 aacgacaaat gcgtgagatg gaggaaaatt tcgccgtaga agctgccaac tatcaggaca
     1921 ctataggtcg cctgcaagac gagattcaga acatgaagga ggagatggcc aggcatctca
     1981 gggagtacca ggatctgctc aatgtcaaaa tggccctgga tatcgagatc gcaacttatc
     2041 ggaaactgct ggaaggcgag gagagtagaa tttcccttcc cttgccgaac ttctccagcc
     2101 tgaatcttag ggaaaccaac ctggactcac tgccactggt agacacacac tccaaaagga
     2161 ccctgttgat caagacagtg gaaaccagag atggccaggt gatcaacgaa acaagccagc
     2221 atcacgatga cctggagggc gggtcaggcg gcagcgtgtc aaagggagaa gagttgttta
     2281 ccggtgttgt tcccatactg gtggaactgg atggtgacgt caatggacac aagtttagcg
     2341 tttctggcga aggagaaggc gatgctacct atggcaaact gaccctcaaa ctgatctgta
     2401 cgactggaaa gttgcctgta ccttggccaa cactggtcac aaccttgggc tatgggctgc
     2461 aatgctttgc ccgttatcct gatcacatga agcaacatga cttctttaag tctgctatgc
     2521 cagagggata tgtgcaggaa cggactatct tcttcaagga cgacggcaac tacaagacga
     2581 gagctgaggt gaaatttgag ggtgacacac tggtgaatcg catagagctg aaaggcatcg
     2641 acttcaaaga agatgggaat attctcgggc ataaactcga gtacaactac aattcccaca
     2701 acgtgtacat cactgccgat aagcagaaga atgggattaa ggcgaacttc aagatccgac
     2761 acaacatcga ggatggtggg gtccagcttg ccgaccatta tcagcagaac acacccattg
     2821 gggatggacc agtcctgttg ccggataacc actacctcag ttaccagagc aaactgtcca
     2881 aagatcccaa tgagaagagg gaccatatgg tactgctcga attcgtgact gcagcaggca
     2941 ttacccttgg gatggatgaa ctttataagt caggctctga ttaaggcgcc aggtacatct
     3001 ccaatacggc gtcttgcata gagaacacga ttagcacacc ggttacccac gtgatgcccg
     3061 aacggaccca tgtcatgcca gagcgcactg gtaacttgtt tattgcagct tataatggtt
     3121 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3181 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtcgtcga attcgagttg
     3241 gcgcgcctgt cattctaaat ctctctttca gcctaaagct ttttccccgt atccccccag
     3301 gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct
     3361 tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga
     3421 ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca
     3481 tccctggggg ctttgggagg gggctgtccc cgtgagctcc caggcgcgcc tgtcattcta
     3541 aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa
     3601 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     3661 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     3721 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     3781 ggggggctgt ccccgtgagc tcccaggcgc gccaactcgg tacctcaccc gattctcatc
     3841 atggagacac atcaggacat ctccaatacc gccatggaaa cgcaccaaga tgcgactgct
     3901 cttctgccag tgacggcatt cgtctgcaag attaacggca tacagaaaga aacgatgaga
     3961 cccattcagg acgagcctca gactccagcg taactggact gcaatcaact cactggctca
     4021 ccttcacggg tgggcctttc ttcggtagaa aatcaaagga tcttcttgag atcctttttt
     4081 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4141 gccggatcaa gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata
     4201 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4261 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4321 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4381 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4441 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4501 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4561 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg
     4621 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcaga aaggcccacc
     4681 cgaaggtgag ccaggtgatt acatttgggc cctcattacc aatgcttaat cagtgaggca
     4741 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4801 ataactacga tgcgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa
     4861 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccgggag ggccgagcgc
     4921 agaagtgatc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4981 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5041 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5101 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5161 gttgccagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5221 tctcttactg tcatgccatc cgtgagatgc ttttctgtga ctggtgagta ctcaaccaag
     5281 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5341 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5401 cgtaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5461 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5521 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatttta
     5581 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     5641 tcattatggt gaaagttgga acctcttacg tgccgatcaa gtcaaaagcc tccggtcgga
     5701 ggcttttga
//
