LOCUS       sequence-273483-        3992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr556.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr556
FEATURES             Location/Qualifiers
     source          1..3992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     76..98
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     90..107
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     91..107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     114..133
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        114..132
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      163..172
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      167..176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1247..1265)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1248..1265)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1283..1299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1283..1299)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1296..1318)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1307..1323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1331..1337,1338..1355,1356..1361))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1376..1397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1514..1531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1685..2273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1765..1784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2444..3235,3236..3304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3067..3086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3305..3409)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3487..3942)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3624..3645)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3836..3855
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
       61 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
      121 actcactata gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg
      181 ggaattcgat tatgctgggg ccatcctaca accacacaat ggaatcccct ggcactttct
      241 tcctcttggg catcccaggg tttcagtctt cttatctttg gctggccatc tcactgagta
      301 ccatgtatag catagctgtc ttaggaaata tgctcattat cattgtgatc tgcatggatt
      361 ccactctaca agagcctatg tacttctttc tatgtgttct ggctgctgtg gatattgtta
      421 tggcttcttc tgtggtacct aagatggtga gcatcttcag ttcaggagac agctccatca
      481 gctttaatgc ctgtttcact cagatgtact ttgtccatgc ggccactgct gtggagacag
      541 gtctgctact ggccatggct tttgatcgct acgtggccat ctgcaagccc ttacattaca
      601 tgagaattct cacccgtcat gtgatgctgg gaattagtgt gactatcacc gttagagctg
      661 ttatttttat gactcctttg agttggatgt tgagtcattt gccattttgt gcctccaatg
      721 tagttcctca ttcctattgt gaacacatgg ctgtggccaa gttagcatgt gctgacccca
      781 tgcccagcag tctctacagt ttgatttttt cctccatcat tgtgggctca gatgtggcct
      841 tcatttcagc ctcttattct ttgattctca aggcagtttt tggactgtcc tcaaggaatg
      901 cacagtggaa ggctttgagt acatgtggct ctcatgttgg ggtcatggct ctgtactatc
      961 tccctgggat ggcatccatc tatgtggcct ggctagggca ggaccgagtt ccactgcaca
     1021 ctcaagtgct attagctgac ttgtacctga tcatcccacc caccttaaac cctatcattt
     1081 atggtattag gaccagacaa atccgggagc ggatatggag tctgctgaca cattgcttct
     1141 tttcccagtg cactcaaggt tcatgaaatc actagtgaat tcgcggccgc ctgcaggtcg
     1201 accatatggg agagctccca acgcgttgga tgcatagctt gagtattcta tagtgtcacc
     1261 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     1321 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1381 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1441 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1501 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1561 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1621 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2101 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2581 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3301 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3361 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3421 gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     3481 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     3541 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     3601 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     3661 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     3721 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3781 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3841 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3901 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3961 gcgcaactgt tgggaagggc gatcggtgcg gg
//
