LOCUS       sequence-273485-        9921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged synaptic vesicle protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..9921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             73..706
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    753..878
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1375..1608
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1793..1837
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2100..2215
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2409..3591
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2640..3582
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3539..3559
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3646..3655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3652..3654,3655..3657,3658..4368)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3652..3654,3655..3657,3658..4368)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3697..3718)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3958..3977)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4305..4326
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4743..5317,5318..5320,5321..5329)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4910..4927)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5137..5156
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5349..5948
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5349..5368)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5845..5865
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5962..6550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6015..6035)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6433..6444)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6757..7390
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7518..7534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7518..7534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7531..7553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7542..7558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7566..7572,7573..7590,7591..7596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7611..7632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7749..7766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7920..8508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8000..8019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8679..9470,9471..9539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9302..9321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9540..9644)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9692..9826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9729..9748)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9783..9802
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
       61 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
      121 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
      181 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
      241 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
      301 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
      361 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
      421 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
      481 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
      541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      721 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      781 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      841 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      901 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      961 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1021 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1081 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1141 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1201 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1261 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1321 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1381 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1441 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1501 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1561 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1621 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1681 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1741 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1801 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1861 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1921 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1981 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2041 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct
     2101 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2161 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2221 tttattacag ggacagcaga gatccagttt atcgatgagt aattcataca aaaggactcg
     2281 cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta gaacccagag
     2341 atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt ggagaagagc
     2401 atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2701 cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     2761 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     2821 gttgaggcct ggcttgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     2881 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     2941 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     3001 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     3061 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     3121 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     3181 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     3241 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     3301 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     3361 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     3421 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     3481 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     3541 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggatctatt
     3601 tccggtgaat tcctcgagac tagttctaga gcggccgcac cggtcgccac catggtgagc
     3661 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     3721 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     3781 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     3841 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     3901 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3961 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     4021 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     4081 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     4141 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     4201 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     4261 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     4321 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ctcagatctc
     4381 gacatgtctg ctaccgctgc cacggccccc cctgctgccc cggctgggga gggtggtccc
     4441 cctgcacccc ctccaaacct caccagtaac aggagactgc agcagaccca ggcccaggtg
     4501 gatgaggtgg tggacatcat gagggtgaac gtggacaagg tcctggagcg agaccagaag
     4561 ctgtcggagc tggacgaccg tgcagatgca ctccaggcgg gggcctccca gtttgaaaca
     4621 agcgcagcca agctcaagcg caaatactgg tggaaaaacc tcaagatgat gatcatcttg
     4681 ggagtgattt gcgccatcat cctcatcatc atcatagttt acttcagcac ttaaggatcc
     4741 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     4801 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     4861 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     4921 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     4981 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5041 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5101 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5161 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5221 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5281 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac
     5341 gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     5401 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5461 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5521 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5581 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5641 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5701 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5761 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5821 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5881 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5941 gtgcctagac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     6001 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6061 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6121 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6181 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6241 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6301 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6361 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6421 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6481 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6541 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     6601 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6661 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6721 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     6781 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     6841 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     6901 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     6961 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     7021 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     7081 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     7141 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     7201 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7261 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7321 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7381 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7441 tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag
     7501 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7561 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7621 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7681 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7741 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7801 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7861 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7921 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7981 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8041 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8101 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8161 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8221 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8281 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8341 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8401 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8461 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8521 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8581 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8641 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8701 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8761 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8821 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8881 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8941 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9001 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9061 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9121 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9181 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9241 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9301 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9361 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9421 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9481 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9541 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9601 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9661 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
     9721 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9781 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     9841 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     9901 gtggtttcat ttactctaaa c
//
