LOCUS       sequence-273487-        4745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Acsm4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-OMACS
FEATURES             Location/Qualifiers
     source          1..4745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     146..165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     399..416
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    533..554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(569..574,575..592,593..599)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    607..623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     612..634
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     631..647
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     631..647
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        665..683
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     665..682
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      2507..2516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2511..2520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2550..2569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2551..2569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2576..2593)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2576..2592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2585..2607)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2733..3188
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2820..2839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3030..3051
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3266..3370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3371..3439,3440..4231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3589..3608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4402..4745,1..245)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
       61 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      121 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      181 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      241 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      301 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      361 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      421 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      481 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      541 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      601 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      661 agctatttag gtgacactat agaatactca agctatgcat ccaacgcgtt gggagctctc
      721 ccatatggtc gacctgcagg cggccgcgaa ttcactagtg attatgaagg ttctcctcca
      781 ctgtcagaga cttagattca tctggcttgc caagccagcc ggccggcact tccacagaga
      841 ttcccagctc tgggcacctc tgacactgga tgactttgaa gccatcaatc gctgtgagaa
      901 gccattaccc aagaacttta actttgcagc agatgttctg gaccagtggt ccctaaagga
      961 gaagtctgga gagaggccag ccaacccagc cctgtggtgg gtgaatggga aaggagatga
     1021 ggtgaagtgg agcttccaag aactgggttc cttgtcccga aaggctacca atgtgctcac
     1081 caagccctgc ggcctgcaga gaggagaccg ggtggctgtg attctgcccc gtatccctga
     1141 atggtggctc atcaatgtgg cttgcatgag aacagggctt gtattcatgc caggaacgat
     1201 ccaactgacc agaaaagaca tcctctaccg gctgcaagca tccaaggcca agtgcattgt
     1261 ggctagtgag gaagtggccc ccgcagtgga ctccattgta tcagagtgtc ccagcctgaa
     1321 gactaaactc ctggtgtctc cacaccactg ggatggatgg ctcaactttc aggagttact
     1381 tcaatcggcc tctgaagagc acaattgtgt ggagacggga agtcaagaac caatggccat
     1441 ttacttcacc agtgggacca caggctcccc caagatggct cagcactcac agggcagcct
     1501 tggcattggg tatacccttt gtggaaggta ctggctggac ttgacatcct cagatatcat
     1561 gtggaacatg tcagacactg gctggatcaa ggctgccatt ggtagtgtgt tttctacatg
     1621 gcttcgaggg gcctgtgttt ttgtgcaccg gatggcccag ttcgacactg atatcttcct
     1681 agacacactt accacatatc ccatcacgac cctgtgcagt gctcctacgg tgtaccggat
     1741 gctcgtacaa aaggacctta agagatatca attcaagagg ctgcggcact gcctgacagg
     1801 aggggagcca ctcaaccccg aagtgctgga acagtggaag atgcaaactg ggctggagct
     1861 gtacgagggc tacggacaga ccgaagtggg aataatttgc gccaatcgca aaggagaagc
     1921 aattaaacca ggttccatgg gaaaaggagt agtgccgtac gatgtgcaga ttatagatga
     1981 aaatggaaat atcctaccat ccggcaagga aggggaaatt gcccttaggc tgaagtccga
     2041 tcggcccttc tgtttcttct ctgaatatgt ggacaatcca gaaaagacag atgccacaat
     2101 aagaagaaat ttttatgtca ctggagacag aggagtgatg gatgatgacg gatacttttg
     2161 gtttgtcggc agagctgatg atgtcattat atcttctggg taccgaattg ggccttttga
     2221 agtagagagc gcactgatag agcacccagc agtagttgag tcagctgttg tcagcagtcc
     2281 agatccgatc cgaggagagg tggtgaaggc atttattgta ctagctgcac cctacaagtg
     2341 ctccaaccgt gagaagttaa ccgctgaact tcaggatcat gtgaaaaatt caacagcacc
     2401 ttacaaatac ccaagaaaag tggaatttgt tcaggaactc ccaaagacaa tcactgggaa
     2461 aatcaaacgc aacgtgttaa gagaccagga aaatcgaatt cccgcggccg ccatggcggc
     2521 cgggagcatg cgacgtcggg cccaattcgc cctatagtga gtcgtattac aattcactgg
     2581 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2641 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2701 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc
     2761 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2821 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2881 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2941 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3001 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3061 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3121 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     3181 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc
     3241 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3301 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3361 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3421 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3481 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3541 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3601 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3661 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3721 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3781 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3841 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3901 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3961 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4021 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4081 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4141 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4201 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4261 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4321 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4381 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4441 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4501 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4561 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4621 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4681 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4741 gccca
//
