LOCUS       sequence-273490-        4398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-020   NLSNoLS-Neptune//BsaI
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     CDS             843..890
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    1782..1903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1819..1838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1873..1892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2704..2733
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2755..3342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3243..3262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3354..3381)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(3405..4196,4197..4265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4028..4047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4266..4368)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(4369..4398,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggsgrggg gsggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgaaacggcc tgctgccacc aagaaagcag gacaggccaa gaagaagaaa cgcaggagaa
      901 ggcgacgtag aggcggtagc gggggcagcg tcagcaaagg cgaggagctc atcaaggaga
      961 acatgcacac caagctgtac atggaaggta ctgtgaataa ccaccacttc aaatgcacgc
     1021 atgaaggtga aggcaagccc tatgagggca cacagaccaa tcgcatcaaa gtggttgagg
     1081 gaggtcctct gccgtttgcg ttcgacattc tggccacatg cttcatgtat ggctccaaaa
     1141 cgttcatcaa ccacactcag ggcatacccg atttcttcaa gcagtctttt cctgaaggct
     1201 ttacatggga gagagtgacc acatacgaag atggaggcgt cctcactgtg acacaagaca
     1261 caagtctgca ggatggatgt ctcatctaca atgtgaagct gcgtggggtg aactttccct
     1321 ctaatgggcc tgtaatgcag aagaaaactc ttgggtggga agcatcaacc gagactcttt
     1381 acccagctga tggagggctt gaaggccggt gtgacatggc tctcaagttg gtaggtggag
     1441 gacatctgca ttgcaacctg aaaaccacct acaggagcaa gaagccagca aagaacctga
     1501 agatgccagg agtctatttt gtggacagac gattggagcg gattaaggag gccgacaatg
     1561 aaacctatgt cgagcaacac gaggttgccg ttgctaggta ttgtgacttg ccttccaaac
     1621 tggggcataa gctgaacggg atggatgagc tgtacaaatc aggctctgat taaggcgcca
     1681 ggtacatctc caatacggcg tcttgcatag agaacacgat tagcacaccg gttacccacg
     1741 tgatgcccga acggacccat gtcatgccag agcgcactgg taacttgttt attgcagctt
     1801 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1861 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtcgtcgaa
     1921 ttcgagttgg cgcgcctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
     1981 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     2041 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     2101 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     2161 gtaattacat ccctgggggc tttgggaggg ggctgtcccc gtgagctccc aggcgcgcct
     2221 gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc
     2281 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
     2341 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
     2401 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
     2461 ggctttgggg gggggctgtc cccgtgagct cccaggcgcg ccaactcggt acctcacccg
     2521 attctcatca tggagacaca tcaggacatc tccaataccg ccatggaaac gcaccaagat
     2581 gcgactgctc ttctgccagt gacggcattc gtctgcaaga ttaacggcat acagaaagaa
     2641 acgatgagac ccattcagga cgagcctcag actccagcgt aactggactg caatcaactc
     2701 actggctcac cttcacgggt gggcctttct tcggtagaaa atcaaaggat cttcttgaga
     2761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aggtaactgg cttcagcaga
     2881 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2941 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3001 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3061 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3121 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3181 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcat
     3301 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcagaa
     3361 aggcccaccc gaaggtgagc caggtgatta catttgggcc ctcattacca atgcttaatc
     3421 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3481 gtcgtgtaga taactacgat gcgggagggc ttaccatctg gccccagtgc tgcaatgata
     3541 ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccgggagg
     3601 gccgagcgca gaagtgatcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3661 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3721 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3781 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3841 cctccgatcg ttgccagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3901 ctgcataatt ctcttactgt catgccatcc gtgagatgct tttctgtgac tggtgagtac
     3961 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4021 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4081 tcttcggggc gtaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4141 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4201 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4261 ctcattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     4321 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaag tcaaaagcct
     4381 ccggtcggag gcttttga
//
