LOCUS       sequence-273491-        4524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Ncam2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-OCAM
FEATURES             Location/Qualifiers
     source          1..4524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..95
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     87..104
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     88..104
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     111..130
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        111..129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      164..173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1776..1794)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1777..1794)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1812..1828)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1812..1828)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1825..1847)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1836..1852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1860..1866,1867..1884,1885..1890))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1905..1926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2043..2060)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2214..2802)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2294..2313)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2973..3764,3765..3833))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3596..3615
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3834..3938)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4016..4471)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4153..4174)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4365..4384
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
       61 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
      121 cactataggg cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga
      181 attcgattga gaagtggtgt cccctcaaga gttcaagcaa ggggaggatg cagaagtggt
      241 ttgcagagtg agcagttccc cagcccctgc ggtcagctgg ttgtaccaca atgaggaagt
      301 caccaccatc cccgacaatc ggtttgcagt gcttgcaaac aataatttgc agatcctcaa
      361 tatcaataaa agtgatgaag gtatatacag atgtgaagga agagtggagg cgaggggaga
      421 gattgacttc cgggatatca ttgttattgt taacgttcca ccagcaatca tgatgcccca
      481 gaagtccttc aatgctacag cagagagagg agaagagatg accttaacat gcaaggcctc
      541 gggctcccca gatccaacca tctcttggtt caggaacggc aaactcattg aagaaaatga
      601 aaagtatatt ttaaagggca gtaatacaga gctcactgtc aggaacataa tcaataaaga
      661 cgggggctct tatgtctgca aagcaacaaa caaggcagga gaagatcaaa agcaggcctt
      721 ccttcaagtc tttgtacagc ctcatatatt acaacttaaa aatgagacaa catctgagaa
      781 tggtcatgtc acactcgtat gtgaagcaga aggggagcct gttccagaaa tcacatggaa
      841 aagagccata gatggagtca tgttttctga aggtgataag agtccggatg gccgcatcga
      901 agttaaaggg cagcatggac gctcttcact gcacattaga gatgtgaagt tgtcagattc
      961 ggggagatat gactgtgagg ccgctagtcg gattggtggg caccagagga gcatgcacct
     1021 ggacatcgaa tatgctccta agtttgtttc aaatcagaca atgtattact cttgggaagg
     1081 aaatccaatc aacataagtt gtgatgtgac agcaaaccca cctgcgtcaa tccactggag
     1141 aagagagaaa ctgctcttac cagctaagaa tacgactcat ttaaaaactc acagtgtagg
     1201 aagaaagatg atactcgaga ttgcccccac atcagacaat gactttggac gatataactg
     1261 cacagctact aaccgtatag gcacaagatt tcaggaatat attcttgaat tagcagatgt
     1321 cccctctagt ccccatggag tgaagattat agagctgtca cagaccacag ccaagatctc
     1381 tttcaataaa cccgaatccc atggaggtgt gcctattcat cactatcaag tggatgtcaa
     1441 agaagtggca tcagaaacct ggaaaatagt acgctcccat ggagttcaaa cgatggttgt
     1501 tttgagcagt ctggaaccaa atacgactta cgaaattagg gttgcagcag tgaatggcaa
     1561 aggtcaagga gactacagta aaatagaaat attccagaca ctgccagtcc gtgagccaag
     1621 tcccccttcc atacatggac agccaagcag tggcaagagt tttaaaatca gcatcaccaa
     1681 gcaagatgat ggaggaatca ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga
     1741 gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg
     1801 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     1861 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     1921 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1981 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2041 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2101 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2161 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2221 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2281 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2341 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2401 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2461 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2521 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2581 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2641 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2701 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2761 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2821 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2881 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2941 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3001 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3061 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3121 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3181 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3241 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3301 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3361 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3421 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3481 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3541 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3601 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3661 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3721 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3781 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3841 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3901 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3961 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     4021 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4081 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4141 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4201 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4261 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4321 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4381 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4441 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     4501 gggaagggcg atcggtgcgg gcct
//
