LOCUS       sequence-273499-        9921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged plasma membrane marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..9921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..1188
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          237..1179
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1136..1156
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1243..1252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1249..1251,1252..1254,1255..1965)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1249..1251,1252..1254,1255..1965)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1294..1315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1555..1574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1902..1923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2340..2914,2915..2917,2918..2926)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2507..2524)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2734..2753
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2946..3545
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2946..2965)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3442..3462
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3559..4147
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3612..3632)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4030..4041)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4354..4987
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5115..5131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5115..5131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5128..5150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5139..5155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5163..5169,5170..5187,5188..5193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5208..5229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5346..5363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5517..6105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5597..5616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6276..7067,7068..7136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6899..6918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7137..7241)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7289..7423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7326..7345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7380..7399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7591..8224
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8271..8396
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8893..9126
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9311..9355
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9618..9733
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
       61 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      121 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      181 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      241 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      301 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      361 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      421 gaggcctggc ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      481 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      541 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      601 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      661 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      721 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
      781 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
      841 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
      901 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
      961 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1021 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1081 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1141 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg atctatttcc
     1201 ggtgaattcc tcgagactag ttctagagcg gccgcaccgg tcgccaccat ggtgagcaag
     1261 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1321 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1381 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1441 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1501 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1561 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1621 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1681 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1741 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1801 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1861 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1921 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtactc agatctcgac
     1981 atggcaggaa tagagttgga gcggtgccag cagcaggcga acgaggtgac ggaaattatg
     2041 cgtaacaact tcggcaaggt cctggagcgt ggtgtgaagc tggccgaact gcagcagcgt
     2101 tcagaccaac tcctggatat gagctcaacc ttcaacaaga ctacacagaa cctggcccag
     2161 aagaagtgct gggagaacat ccgttaccgg atctgcgtgg ggctggtggt ggttggtgtc
     2221 ctgctcatca tcctgattgt gctgctggtc gtctttctcc ctcagagcag tgacagcagt
     2281 agtgccccac ggacccagga tgcaggcatt gcctcagggc ctgggaactg aggatcccgc
     2341 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     2401 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     2461 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     2521 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     2581 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     2641 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     2701 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     2761 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     2821 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     2881 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac
     2941 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     3001 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     3061 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     3121 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     3181 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     3241 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     3301 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     3361 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     3421 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     3481 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     3541 cctagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3601 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3661 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3721 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3781 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3841 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3901 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     3961 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4021 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4081 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4141 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     4201 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     4261 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     4321 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     4381 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     4441 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     4501 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     4561 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     4621 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     4681 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     4741 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     4801 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4861 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4921 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4981 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5041 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
     5101 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5161 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5221 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5281 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5341 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5401 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5461 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5521 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5581 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5641 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5701 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5761 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5821 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5881 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5941 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6001 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6061 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6121 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6181 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6241 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6301 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6361 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6421 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6481 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6541 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6601 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6661 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6721 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6781 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6841 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6901 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6961 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7021 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7081 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7141 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7201 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7261 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
     7321 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7381 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     7441 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     7501 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     7561 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
     7621 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     7681 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     7741 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     7801 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     7861 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     7921 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     7981 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     8041 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8101 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8161 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8221 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     8281 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     8341 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     8401 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     8461 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     8521 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     8581 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     8641 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8701 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     8761 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8821 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8881 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8941 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     9001 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9061 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9121 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9181 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9241 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9301 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9361 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9421 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9481 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     9541 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     9601 atctcgacgg tatcgccttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     9661 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     9721 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttat cgatgagtaa
     9781 ttcatacaaa aggactcgcc cctgccttgg ggaatcccag ggaccgtcgt taaactccca
     9841 ctaacgtaga acccagagat cgctgcgttc ccgccccctc acccgcccgc tctcgtcatc
     9901 actgaggtgg agaagagcat g
//
