LOCUS       sequence-273502-        5538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5538)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-022   Lamp1-Eos//BsaI
FEATURES             Location/Qualifiers
     source          1..5538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2922..3043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2959..2978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3013..3032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3844..3873
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3895..4482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4383..4402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4494..4521)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4545..5336,5337..5405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5168..5187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5406..5508)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5509..5538,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggs ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tggccgcacc cggtgcaaga cgccctcttc tgctgctgct gctggccggc ctcgctcact
      901 cagctcccgc actctttgag gtgaaagaca acaacggaac tgcgtgtatt atggcatctt
      961 tcagcgcttc attcctgaca acgtatgaag cggggcatgt cagtaaagtg agcaacatga
     1021 ctctgccagc cagtgctgaa gtgctgaaga actccagctc atgcggggaa aagaacgcct
     1081 cagaaccaac cctcgctata acctttgggg agggctactt gttgaagttg acgtttacga
     1141 agaacacaac acggtattcc gtacagcaca tgtacttcac ttacaacctg agcgatactc
     1201 agttctttcc caatgcaagt tccaagggcc cagacactgt tgattccacc accgacatca
     1261 aggccgacat caacaagaca tacagatgcg tgtctgacat ccgggtgtac atgaagaatg
     1321 tcactattgt actctgggat gccaccatac aggcctattt gcccagcagc aatttcagca
     1381 aagaggaaac aaggtgtccg caggatcagc catctcctac cactggacct cctagtccat
     1441 ccccaccgct tgtccctaca aaccccagcg tgagcaaata caacgtcaca ggagacaatg
     1501 gcacctgtct gctggcatcc atggctttgc agctgaatat cacctatatg aagaaggaca
     1561 atacgaccgt gactcgagcc tttaacatca atccttctga caaatacagc ggtacctgtg
     1621 gggctcaact ggtgacactc aaagtcggta acaaatctcg cgtgctggaa ctgcagtttg
     1681 gcatgaatgc cacctcctct ctgttcttcc tgcaaggtgt gcagctgaat atgacccttc
     1741 ctgatgccat tgagcccaca ttctccacca gcaactatag tctgaaggcg cttcaagcta
     1801 gcgttgggaa cagttacaaa tgcaattctg aggagcacat tttcgtgtca aaggcccttg
     1861 ctctcaatgt cttttccgtt caggtacagg cattcagagt ggagtctgat cgtttcggct
     1921 cagtagaaga gtgcgtccaa gacggcaata acatgctgat ccccattgcc gttggaggag
     1981 ctcttgccgg actggttctc atcgtgctca ttgcctacct gataggcagg aagaggtcac
     2041 atgcagggta tcagacaatc agcgagtttg ggagtacagg atccactggt tctactggcg
     2101 ctgatggcgg gtcaggcggc agctcagcga tcaaacccga catgaagatc aacctccgaa
     2161 tggaaggcaa cgtaaacggc catcacttcg tgattgatgg cgatggcaca gggaagccat
     2221 ttgaggggaa acaaagcatg gatctggagg tgaaggaagg tggtcctctg ccttttgcct
     2281 tcgacatact gactacagcc tttcactatg ggaatcgcgt gtttgtcgag tatcccgacc
     2341 atatacagga ttacttcaag cagagtttcc cgaaaggcta tagctgggaa aggtccctca
     2401 catttgagga tggagggatt tgcattgcaa gaaacgacat caccatggaa ggagacacct
     2461 tctacaacaa ggttcggttc catgggacaa atttccccgc taatggtcct gtgatgcaga
     2521 agaaaaccct gaagtgggaa ccatccactg agaagatgta cgtgagagat ggagtactga
     2581 cgggtgacat tcacgctgca ctgctgttgg agggcaatgc ccactatcgg tgtgattttc
     2641 gcactaccta caaggccaaa gagaaaggcg tcaaactccc tggataccac cttgtcgatc
     2701 attgcatcga gatcctttct cacgacaagg actacaacaa ggtgaagctg tatgaacatg
     2761 ccgttgctca ctctggattg ccagacaatg caaggcgttc aggctctgat taaggcgcca
     2821 ggtacatctc caatacggcg tcttgcatag agaacacgat tagcacaccg gttacccacg
     2881 tgatgcccga acggacccat gtcatgccag agcgcactgg taacttgttt attgcagctt
     2941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtcgtcgaa
     3061 ttcgagttgg cgcgcctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
     3121 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     3181 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     3241 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     3301 gtaattacat ccctgggggc tttgggaggg ggctgtcccc gtgagctccc aggcgcgcct
     3361 gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc
     3421 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
     3481 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
     3541 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
     3601 ggctttgggg gggggctgtc cccgtgagct cccaggcgcg ccaactcggt acctcacccg
     3661 attctcatca tggagacaca tcaggacatc tccaataccg ccatggaaac gcaccaagat
     3721 gcgactgctc ttctgccagt gacggcattc gtctgcaaga ttaacggcat acagaaagaa
     3781 acgatgagac ccattcagga cgagcctcag actccagcgt aactggactg caatcaactc
     3841 actggctcac cttcacgggt gggcctttct tcggtagaaa atcaaaggat cttcttgaga
     3901 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3961 ggtttgtttg ccggatcaag agctaccaac tctttttccg aggtaactgg cttcagcaga
     4021 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4081 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4141 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4201 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4261 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4321 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4381 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcat
     4441 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcagaa
     4501 aggcccaccc gaaggtgagc caggtgatta catttgggcc ctcattacca atgcttaatc
     4561 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4621 gtcgtgtaga taactacgat gcgggagggc ttaccatctg gccccagtgc tgcaatgata
     4681 ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccgggagg
     4741 gccgagcgca gaagtgatcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4801 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4861 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4921 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4981 cctccgatcg ttgccagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5041 ctgcataatt ctcttactgt catgccatcc gtgagatgct tttctgtgac tggtgagtac
     5101 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5161 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5221 tcttcggggc gtaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5281 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5341 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5401 ctcattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     5461 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaag tcaaaagcct
     5521 ccggtcggag gcttttga
//
