LOCUS       sequence-273507-        3913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Prochlorococcus phage P-SSM2 phage Fd fused to mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Campbell IJ, Olmos JL Jr, Xu W, Kahanda D, Atkinson JT, Sparks ON, 
            Miller MD, Phillips GN Jr, Bennett GN, Silberg JJ
  TITLE     Prochlorococcus phage ferredoxin: Structural characterization and 
            electron transfer to cyanobacterial sulfite reductases.
  JOURNAL   J Biol Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501.
  PUBMED    32434930
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501."
COMMENT     Sequence Label: pFd022.R2.RFP
FEATURES             Location/Qualifiers
     source          1..3913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             163..837
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     terminator      867..938
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      954..981
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1033..1067
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             1102..1728
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1178..1197)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      1734..1820
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1985..2573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2065..2084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2661..2755)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2776..3435)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3350..3369
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3604..3622
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(3643..3661,3662..3667,3668..3684,3685..3690,
                     3691..3716)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    3643..3661
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3668..3686
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcagttaaca atgaggaaca gtctttttta gacgacgatc agctggaaga aggatggtca
       61 ctgctttgcg tggcaatgcc acagtcggac tgtgttatcc tgacggagca agaagacaac
      121 ctggatgggg gctctggagg ttctggaggt tctggtgggt ccgcgagtag cgaagacgtt
      181 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
      241 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
      301 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
      361 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
      421 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
      481 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
      541 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
      601 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
      661 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
      721 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
      781 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaagaa
      841 ttctgataat aatctagacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      961 cttcgggtgg gcctttctgc gtttataggt acccgtggat cctctagagt cgacctgcag
     1021 gcatgcaagc tttttacggc tagctcagtc ctaggtatag tgctagccca gccagagaaa
     1081 cactctttaa cagggggtag tatgatgtct cgtttagata aaagtaaagt gattaacagc
     1141 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     1201 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     1261 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     1321 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     1381 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     1441 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     1501 gcactcagcg cagtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     1561 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     1621 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     1681 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaagg catcaaataa
     1741 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1801 ctctcctgag taggacaaat ccgccgccct agacctaggg cgttcggctg cggcgagcgg
     1861 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcatgca
     1921 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1981 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2041 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2101 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2161 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2221 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2281 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2341 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2401 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2461 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2521 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2581 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2641 tggtcatgac tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     2701 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     2761 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     2821 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     2881 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     2941 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     3001 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccataaca
     3061 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     3121 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     3181 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     3241 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     3301 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     3361 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     3421 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     3481 gaccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgg gccggccaac
     3541 gtctcatttt cgccagatat cgacgtctaa gaaaccatta ttatcatgac attaacctat
     3601 aaaaataggc gtatcacgag gccctttcgt cttcacctcg agtccctatc agtgatagag
     3661 attgacatcc ctatcagtga tagagatact gagcacatca gcaggacgca ctgaccgtat
     3721 aaacggggaa tatagaaaag agggttctat ggcgtatagc attaccctgc gctcgccgga
     3781 tggcgctgaa gaagtggtac agtgcgagga agatcagtat attttagaag cggcggagga
     3841 tgctgggctg gatatgccat caagttgccg tgcgggcgct tgctccgctt gtctcggcaa
     3901 agtgttagag ggg
//
