LOCUS       sequence-273524-        3491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Env gene from HIV-1 LAI in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pLAI-Env
FEATURES             Location/Qualifiers
     source          1..3491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1869..2102
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2287..2331
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2625..2651
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3087..3128
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3138..3155
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3178..3195)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3184..3408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcttggtacc gagctcggat ccagtaccct tcaccgatca agctttaggc atctcctatg
       61 gcaggaagaa gcggagacag cgacgaagac ctcctcaagg cagtcagact catcaagttt
      121 ctctatcaaa gcagtaagta gtacatgtaa tgcaacctat acaaatagca atagcagcat
      181 tagtagtagc aataataata gcaatagttg tgtggtccat agtaatcata gaatatagga
      241 aaatattaag acaaagaaaa atagacaggt taattgatag actaatagaa agagcagaag
      301 acagtggcaa tgagagtgaa ggagaaatat cagcacttgt ggagatgggg gtggaaatgg
      361 ggcaccatgc tccttgggat attgatgatc tgtagtgcta cagaaaaatt gtgggtcaca
      421 gtctattatg gggtacctgt gtggaaggaa gcaaccacca ctctattttg tgcatcagat
      481 gctaaagcat atgatacaga ggtacataat gtttgggcca cacatgcctg tgtacccaca
      541 gaccccaacc cacaagaagt agtattggta aatgtgacag aaaattttaa catgtggaaa
      601 aatgacatgg tagaacagat gcatgaggat ataatcagtt tatgggatca aagcctaaag
      661 ccatgtgtaa aattaacccc actctgtgtt agtttaaagt gcactgattt ggggaatgct
      721 actaatacca atagtagtaa taccaatagt agtagcgggg aaatgatgat ggagaaagga
      781 gagataaaaa actgctcttt caatatcagc acaagcataa gaggtaaggt gcagaaagaa
      841 tatgcatttt tttataaact tgatataata ccaatagata atgatactac cagctatacg
      901 ttgacaagtt gtaacacctc agtcattaca caggcctgtc caaaggtatc ctttgagcca
      961 attcccatac attattgtgc cccggctggt tttgcgattc taaaatgtaa taataagacg
     1021 ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca tggaattagg
     1081 ccagtagtat caactcaact gctgttgaat ggcagtctag cagaagaaga ggtagtaatt
     1141 agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa ccaatctgta
     1201 gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat ccagagggga
     1261 ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc acattgtaac
     1321 attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt aagagaacaa
     1381 tttggaaata ataaaacaat aatctttaag caatcctcag gaggggaccc agaaattgta
     1441 acgcacagtt ttaattgtgg aggggaattt ttctactgta attcaacaca actgtttaat
     1501 agtacttggt ttaatagtac ttggagtact gaagggtcaa ataacactga aggaagtgac
     1561 acaatcacac tcccatgcag aataaaacaa tttataaaca tgtggcagga agtaggaaaa
     1621 gcaatgtatg cccctcccat cagcggacaa attagatgtt catcaaatat tacagggctg
     1681 ctattaacaa gagatggtgg taataacaac aatgggtccg agatcttcag acctggagga
     1741 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1801 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1861 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1921 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1981 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     2041 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     2101 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2161 agttggagta ataaatctct ggaacagatt tggaataaca tgacctggat ggagtgggac
     2221 agagaaatta acaattacac aagcttaata cattccttaa ttgaagaatc gcaaaaccag
     2281 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2341 tttaacataa caaattggct gtggtatata aaaatattca taatgatagt aggaggcttg
     2401 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2461 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2521 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcc
     2581 ttagcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca ccgcttgaga
     2641 gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg gtgggaagcc
     2701 ctcaaatatt ggtggaatct cctacagtat tggagtcagg aactaaagaa tagtgctgtt
     2761 agcttgctca atgccacagc catagcagta gctgagggga cagatagggt tatagaagta
     2821 gtacaaggag cttgtagagc tattcgccac atacctagaa gaataagaca gggcttggaa
     2881 aggattttgc tataagatgg gtggcaagtg gtcaaaaagt agtgtggttg gatggcctac
     2941 tgtaagggaa agaatgagac gagctgagcc agcagcagat ggggtgggag cagtatctcg
     3001 agacggatcc agctaagggt caagacaatt ctgcagatat ccagcacagt ggcggccgct
     3061 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     3121 attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     3181 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3241 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3301 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3361 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     3421 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     3481 cggcgggtgt g
//
