LOCUS       sequence-273525-        3469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pYU-2-Env
FEATURES             Location/Qualifiers
     source          1..3469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1819..2052
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2575..2601
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3067..3108
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3118..3135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3158..3175)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3164..3389
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcttggtac cgagctcgga tccagtaccc ttcaccgatc aagctttagg catctcctat
       61 ggcaggaaga agcggagaca gcgacgaaga cctcctcagg acagtcagac tcatcaaagt
      121 tctctatcaa agcagtaagt agtacatgta atgcaatctt tacaagtatt agcaatagta
      181 gcattagtag tagcaacaat aatagcaata gttgtgtgga ccatagtatt catagaatat
      241 aggaaaatat taagacaaag gaaaatagac aggttaatta atagaataac agaaagagca
      301 gaagacagtg gcaatgagag cgacggagat caggaagaat tatcagcact tgtggaaagg
      361 gggcaccttg ctccttggga tgttgatgat ctgtagtgct gcagaacaat tgtgggtcac
      421 agtctattat ggggtacctg tgtggaaaga agcaaccacc actctatttt gtgcatcaga
      481 tgctaaagca tatgatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac
      541 agaccccaac ccacaagaag taaaattgga aaatgtgaca gaaaatttta acatgtggaa
      601 aaataacatg gtagaacaaa tgcatgagga tataatcagt ttatgggatc aaagcctaaa
      661 gccatgtgta aaattaactc cactctgtgt tactttaaat tgcactgatt taaggaatgc
      721 tactaatacc actagtagta gctgggaaac gatggagaaa ggagaaataa aaaactgctc
      781 tttcaatatc accacaagca taagagataa ggtacagaaa gaatatgcac ttttttataa
      841 ccttgatgta gtaccaatag ataatgctag ctataggttg ataagttgta acacctcagt
      901 cattacacag gcctgtccaa aggtatcctt tgagccaatt cccatacatt attgtgcccc
      961 ggctggtttt gcgattctaa aatgtaatga taaaaagttc aatggaacag gaccatgtac
     1021 aaatgtcagc acagtacaat gtacacatgg aattaggcca gtagtatcaa ctcaactgct
     1081 gttaaatggc agtctagcag aagaagagat agtaattaga tctgaaaatt tcacaaacaa
     1141 tgctaaaact ataatagtac agctgaacga atctgtagta attaattgta caagacccaa
     1201 caacaataca agaaaaagta taaatatagg accagggaga gcattgtata caacaggaga
     1261 aataatagga gatataagac aagcacattg taaccttagt aaaacacaat gggaaaacac
     1321 tttagaacag atagctataa aattaaaaga acaatttggg aataataaaa caataatctt
     1381 taatccatcc tcaggagggg acccagaaat tgtaacacac agttttaatt gtggagggga
     1441 atttttctac tgtaattcaa cacaactgtt tacttggaat gatactagaa agttaaataa
     1501 cactggaaga aatatcacac tcccatgtag aataaaacaa attataaata tgtggcagga
     1561 agtaggaaaa gcaatgtatg cccctcccat cagaggacaa attagatgtt catcaaatat
     1621 tacagggctg ctattaacaa gagatggtgg taaggacacg aacgggactg agatcttcag
     1681 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1741 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1801 aaaaagagca gtgggactag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1861 tatgggcgca gcgtcaataa cgctgacggt acaggccaga caattattgt ctggtatagt
     1921 gcaacagcag aacaatctgc tgagggctat tgaggcgcaa cagcacctgt tgcaactcac
     1981 agtctggggc atcaagcagc tccaggcaag agtcctggct gtggaaagat acctaaggga
     2041 tcaacagctc ctagggattt ggggttgctc tggaaaactc atttgcacca ctactgtgcc
     2101 ttggaatact agttggagta ataaatctct gaatgaaatt tgggataaca tgacttggat
     2161 gaagtgggaa agagaaattg acaattacac acacataata tactccttaa ttgaacaatc
     2221 gcagaaccaa caagaaaaga atgaacaaga attattggca ttagataaat gggcaagttt
     2281 gtggaattgg tttgacataa caaaatggct gtggtatata aaaatattca taatgatagt
     2341 aggaggcttg ataggtttaa gaatagtttt tgttgtactt tctatagtga atagagttag
     2401 gcagggatac tcaccattat cgtttcagac ccacctccca gctcagaggg gacccgacag
     2461 gcccgacgga atcgaagaag aaggtggaga gagagacaga gacagatccg gtccattagt
     2521 ggatggcttc ttagcaatta tctgggtcga cctacggagc ctgtgccttt tcagctacca
     2581 ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg
     2641 gtggggagtc ctcaaatatt ggtggaatct cctccagtat tggattcagg aactaaagaa
     2701 tagtgctgtt agcttgctca acgccacagc tatagcagta gctgagggaa cagatagggt
     2761 tatagaaata ttacaaagag cttttagagc tgttcttcac atacctgtaa gaataagaca
     2821 gggcttggaa agagctttgc tataagatgg gtggcaagtg gtcaaaacgt agtatggctg
     2881 gatggcctac tgtaagggaa agaatgagac gagccgagcc agcagcagaa agaatgagac
     2941 gagctgagcc agcagcagat ggggtgggag cagtatctcg agacggatcc agctaagggt
     3001 caagacaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
     3061 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
     3121 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     3181 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3241 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3301 attctggggg gtgggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3361 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3421 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggg
//
