LOCUS       sequence-273531-        4242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4242)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-031   EB3-Eos//BsaI
FEATURES             Location/Qualifiers
     source          1..4242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1626..1747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1663..1682)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1717..1736
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2548..2577
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2599..3186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3087..3106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3198..3225)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(3249..4040,4041..4109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3872..3891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4110..4212)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(4213..4242,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgggctgcat caagagcaag aggaaagacg tggaaaacgt cgagggcggc agctcagcga
      901 tcaaacccga catgaagatc aacctccgaa tggaaggcaa cgtaaacggc catcacttcg
      961 tgattgatgg cgatggcaca gggaagccat ttgaggggaa acaaagcatg gatctggagg
     1021 tgaaggaagg tggtcctctg ccttttgcct tcgacatact gactacagcc tttcactatg
     1081 ggaatcgcgt gtttgtcgag tatcccgacc atatacagga ttacttcaag cagagtttcc
     1141 cgaaaggcta tagctgggaa aggtccctca catttgagga tggagggatt tgcattgcaa
     1201 gaaacgacat caccatggaa ggagacacct tctacaacaa ggttcggttc catgggacaa
     1261 atttccccgc taatggtcct gtgatgcaga agaaaaccct gaagtgggaa ccatccactg
     1321 agaagatgta cgtgagagat ggagtactga cgggtgacat tcacgctgca ctgctgttgg
     1381 agggcaatgc ccactatcgg tgtgattttc gcactaccta caaggccaaa gagaaaggcg
     1441 tcaaactccc tggataccac cttgtcgatc attgcatcga gatcctttct cacgacaagg
     1501 actacaacaa ggtgaagctg tatgaacatg ccgttgctca ctctggattg ccagacaatg
     1561 caaggcgttc aggctaataa cagcttccgg actctagaac atccctacag gtgatatcct
     1621 cgggtaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1681 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1741 tatcttatca tgtctgtcgt cgaattcgag ttggcgcgcc tgtcattcta aatctctctt
     1801 tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     1861 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     1921 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     1981 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg agggggctgt
     2041 ccccgtgagc tcccaggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt
     2101 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
     2161 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
     2221 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
     2281 gagggacgta attacatccc tgggggcttt gggggggggc tgtccccgtg agctcccagg
     2341 cgcgccaact cggtacctca cccgattctc atcatggaga cacatcagga catctccaat
     2401 accgccatgg aaacgcacca agatgcgact gctcttctgc cagtgacggc attcgtctgc
     2461 aagattaacg gcatacagaa agaaacgatg agacccattc aggacgagcc tcagactcca
     2521 gcgtaactgg actgcaatca actcactggc tcaccttcac gggtgggcct ttcttcggta
     2581 gaaaatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2701 tccgaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2761 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2821 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2881 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2941 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3001 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3061 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3121 tttcgccacc tctgacttga gcatcgattt ttgtgatgct cgtcaggggg gcggagccta
     3181 tggaaaaacg ccagcaacgc agaaaggccc acccgaaggt gagccaggtg attacatttg
     3241 ggccctcatt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3301 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatgcggga gggcttacca
     3361 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     3421 gcaataaacc agccagccgg gagggccgag cgcagaagtg atcctgcaac tttatccgcc
     3481 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3541 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3601 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3661 aaaaaagcgg ttagctcctt cggtcctccg atcgttgcca gaagtaagtt ggccgcagtg
     3721 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtgaga
     3781 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3841 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3901 aaagtgctca tcattggaaa acgttcttcg gggcgtaaac tctcaaggat cttaccgctg
     3961 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4021 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4081 agggcgacac ggaaatgttg aatactcatt ttagcttcct tagctcctga aaatctcgat
     4141 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     4201 acgtgccgat caagtcaaaa gcctccggtc ggaggctttt ga
//
