LOCUS       sequence-273533-        5577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5577)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-025   Lamp1-mVenus//BsaI
FEATURES             Location/Qualifiers
     source          1..5577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2427..2446)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2961..3082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2998..3017)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3052..3071
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3883..3912
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3934..4521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4422..4441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4533..4560)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4584..5375,5376..5444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5207..5226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5445..5547)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5548..5577,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggs ggggsgrggg gsggggsggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tggccgcacc cggtgcaaga cgccctcttc tgctgctgct gctggccggc ctcgctcact
      901 cagctcccgc actctttgag gtgaaagaca acaacggaac tgcgtgtatt atggcatctt
      961 tcagcgcttc attcctgaca acgtatgaag cggggcatgt cagtaaagtg agcaacatga
     1021 ctctgccagc cagtgctgaa gtgctgaaga actccagctc atgcggggaa aagaacgcct
     1081 cagaaccaac cctcgctata acctttgggg agggctactt gttgaagttg acgtttacga
     1141 agaacacaac acggtattcc gtacagcaca tgtacttcac ttacaacctg agcgatactc
     1201 agttctttcc caatgcaagt tccaagggcc cagacactgt tgattccacc accgacatca
     1261 aggccgacat caacaagaca tacagatgcg tgtctgacat ccgggtgtac atgaagaatg
     1321 tcactattgt actctgggat gccaccatac aggcctattt gcccagcagc aatttcagca
     1381 aagaggaaac aaggtgtccg caggatcagc catctcctac cactggacct cctagtccat
     1441 ccccaccgct tgtccctaca aaccccagcg tgagcaaata caacgtcaca ggagacaatg
     1501 gcacctgtct gctggcatcc atggctttgc agctgaatat cacctatatg aagaaggaca
     1561 atacgaccgt gactcgagcc tttaacatca atccttctga caaatacagc ggtacctgtg
     1621 gggctcaact ggtgacactc aaagtcggta acaaatctcg cgtgctggaa ctgcagtttg
     1681 gcatgaatgc cacctcctct ctgttcttcc tgcaaggtgt gcagctgaat atgacccttc
     1741 ctgatgccat tgagcccaca ttctccacca gcaactatag tctgaaggcg cttcaagcta
     1801 gcgttgggaa cagttacaaa tgcaattctg aggagcacat tttcgtgtca aaggcccttg
     1861 ctctcaatgt cttttccgtt caggtacagg cattcagagt ggagtctgat cgtttcggct
     1921 cagtagaaga gtgcgtccaa gacggcaata acatgctgat ccccattgcc gttggaggag
     1981 ctcttgccgg actggttctc atcgtgctca ttgcctacct gataggcagg aagaggtcac
     2041 atgcagggta tcagacaatc agcgagtttg ggagtacagg atccactggt tctactggcg
     2101 ctgatggcgg gtcaggcggc agcgtgtcaa agggagaaga gttgtttacc ggtgttgttc
     2161 ccatactggt ggaactggat ggtgacgtca atggacacaa gtttagcgtt tctggcgaag
     2221 gagaaggcga tgctacctat ggcaaactga ccctcaaact gatctgtacg actggaaagt
     2281 tgcctgtacc ttggccaaca ctggtcacaa ccttgggcta tgggctgcaa tgctttgccc
     2341 gttatcctga tcacatgaag caacatgact tctttaagtc tgctatgcca gagggatatg
     2401 tgcaggaacg gactatcttc ttcaaggacg acggcaacta caagacgaga gctgaggtga
     2461 aatttgaggg tgacacactg gtgaatcgca tagagctgaa aggcatcgac ttcaaagaag
     2521 atgggaatat tctcgggcat aaactcgagt acaactacaa ttcccacaac gtgtacatca
     2581 ctgccgataa gcagaagaat gggattaagg cgaacttcaa gatccgacac aacatcgagg
     2641 atggtggggt ccagcttgcc gaccattatc agcagaacac acccattggg gatggaccag
     2701 tcctgttgcc ggataaccac tacctcagtt accagagcaa actgtccaaa gatcccaatg
     2761 agaagaggga ccatatggta ctgctcgaat tcgtgactgc agcaggcatt acccttggga
     2821 tggatgaact ttataagtca ggctctgatt aaggcgccag gtacatctcc aatacggcgt
     2881 cttgcataga gaacacgatt agcacaccgg ttacccacgt gatgcccgaa cggacccatg
     2941 tcatgccaga gcgcactggt aacttgttta ttgcagctta taatggttac aaataaagca
     3001 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3061 ccaaactcat caatgtatct tatcatgtct gtcgtcgaat tcgagttggc gcgcctgtca
     3121 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     3181 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     3241 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     3301 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     3361 ttgggagggg gctgtccccg tgagctccca ggcgcgcctg tcattctaaa tctctctttc
     3421 agcctaaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa
     3481 gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct
     3541 gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc
     3601 tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc
     3661 ccgtgagctc ccaggcgcgc caactcggta cctcacccga ttctcatcat ggagacacat
     3721 caggacatct ccaataccgc catggaaacg caccaagatg cgactgctct tctgccagtg
     3781 acggcattcg tctgcaagat taacggcata cagaaagaaa cgatgagacc cattcaggac
     3841 gagcctcaga ctccagcgta actggactgc aatcaactca ctggctcacc ttcacgggtg
     3901 ggcctttctt cggtagaaaa tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3961 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4021 gctaccaact ctttttccga ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4081 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4141 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4201 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4261 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4321 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4381 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4441 tatagtcctg tcgggtttcg ccacctctga cttgagcatc gatttttgtg atgctcgtca
     4501 ggggggcgga gcctatggaa aaacgccagc aacgcagaaa ggcccacccg aaggtgagcc
     4561 aggtgattac atttgggccc tcattaccaa tgcttaatca gtgaggcacc tatctcagcg
     4621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgatg
     4681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg
     4741 gctccagatt tatcagcaat aaaccagcca gccgggaggg ccgagcgcag aagtgatcct
     4801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgccagaagt
     5041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5101 atgccatccg tgagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg taaactctca
     5281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcattttagc ttccttagct
     5461 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     5521 aagttggaac ctcttacgtg ccgatcaagt caaaagcctc cggtcggagg cttttga
//
