LOCUS       sequence-273537-        5094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5094)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-028   EB3-mVenus//BsaI
FEATURES             Location/Qualifiers
     source          1..5094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2010..2029)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2478..2599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2515..2534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2569..2588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3400..3429
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3451..4038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3939..3958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4050..4077)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4101..4892,4893..4961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4724..4743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4962..5064)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5065..5094,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggsgrggg gsggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tggccgtgaa cgtgtactca accagcgtaa caagcgagaa tctgtcaagg cacgacatgt
      901 tggcttgggt gaacgattcc cttcatctga actacaccaa aatcgaacag ctgtgctctg
      961 gagctgccta ttgccagttt atggacatgc tctttcccgg atgtgtgcac cttcggaaag
     1021 tcaaattcca ggcaaaactg gaacatgagt atatccacaa ctttaaggtt ctgcaagcag
     1081 cttttaagaa gatgggcgtg gacaagatca tcccggtgga aaagctcgtg aaagggaagt
     1141 ttcaggacaa tttcgagttc attcagtggt tcaagaagtt ctttgacgcc aactatgatg
     1201 ggaaagatta caatccactg cttgccagac agggacagga tgtcgcaccg ccacccaatc
     1261 ctggcgacca gatcttcaac aagagcaaga aactgattgg gacagcggtt ccacaacgta
     1321 cttcccctac tggccccaag aacatgcaga cgtctggcag actgagcaat gttgctccac
     1381 cctgtatact ccgcaagaat ccacctagtg ctcgaaatgg cggtcatgag acagatgccc
     1441 agatactgga gctcaaccaa cagctggtag acctgaaact gaccgtcgac ggattggaga
     1501 aagagaggga cttctacttc tccaagttgc gggatataga gctcatttgc caggaacacg
     1561 agagcgaaaa ctctcccgtc atcagtggta tcatcggtat tctgtatgcc actgaagaag
     1621 ggtttgcacc tcctgaagat gacgaaattg aggagcacca acaggaggat caagacgagt
     1681 acctggatgg cgggtcaggc ggcagcgtgt caaagggaga agagttgttt accggtgttg
     1741 ttcccatact ggtggaactg gatggtgacg tcaatggaca caagtttagc gtttctggcg
     1801 aaggagaagg cgatgctacc tatggcaaac tgaccctcaa actgatctgt acgactggaa
     1861 agttgcctgt accttggcca acactggtca caaccttggg ctatgggctg caatgctttg
     1921 cccgttatcc tgatcacatg aagcaacatg acttctttaa gtctgctatg ccagagggat
     1981 atgtgcagga acggactatc ttcttcaagg acgacggcaa ctacaagacg agagctgagg
     2041 tgaaatttga gggtgacaca ctggtgaatc gcatagagct gaaaggcatc gacttcaaag
     2101 aagatgggaa tattctcggg cataaactcg agtacaacta caattcccac aacgtgtaca
     2161 tcactgccga taagcagaag aatgggatta aggcgaactt caagatccga cacaacatcg
     2221 aggatggtgg ggtccagctt gccgaccatt atcagcagaa cacacccatt ggggatggac
     2281 cagtcctgtt gccggataac cactacctca gttaccagag caaactgtcc aaagatccca
     2341 atgagaagag ggaccatatg gtactgctcg aattcgtgac tgcagcaggc attacccttg
     2401 ggatggatga actttataag tcaggctaat aacagcttcc ggactctaga acatccctac
     2461 aggtgatatc ctcgggtaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2521 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2581 aactcatcaa tgtatcttat catgtctgtc gtcgaattcg agttggcgcg cctgtcattc
     2641 taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca
     2701 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
     2761 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
     2821 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
     2881 ggagggggct gtccccgtga gctcccaggc gcgcctgtca ttctaaatct ctctttcagc
     2941 ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
     3001 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
     3061 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
     3121 cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg gctgtccccg
     3181 tgagctccca ggcgcgccaa ctcggtacct cacccgattc tcatcatgga gacacatcag
     3241 gacatctcca ataccgccat ggaaacgcac caagatgcga ctgctcttct gccagtgacg
     3301 gcattcgtct gcaagattaa cggcatacag aaagaaacga tgagacccat tcaggacgag
     3361 cctcagactc cagcgtaact ggactgcaat caactcactg gctcaccttc acgggtgggc
     3421 ctttcttcgg tagaaaatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3481 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3541 accaactctt tttccgaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3601 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3661 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3721 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3781 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3841 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3901 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3961 agtcctgtcg ggtttcgcca cctctgactt gagcatcgat ttttgtgatg ctcgtcaggg
     4021 gggcggagcc tatggaaaaa cgccagcaac gcagaaaggc ccacccgaag gtgagccagg
     4081 tgattacatt tgggccctca ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatgcgg
     4201 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
     4261 ccagatttat cagcaataaa ccagccagcc gggagggccg agcgcagaag tgatcctgca
     4321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4381 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgc cagaagtaag
     4561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4621 ccatccgtga gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4741 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgtaa actctcaagg
     4801 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4861 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4921 aaaaagggaa taagggcgac acggaaatgt tgaatactca ttttagcttc cttagctcct
     4981 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     5041 ttggaacctc ttacgtgccg atcaagtcaa aagcctccgg tcggaggctt ttga
//
