LOCUS       sequence-273538-       11124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged ERGIC marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..11124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     150..169
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             362..961
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(362..381)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     858..878
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    975..1563
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1028..1048)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1446..1457)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1770..2403
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2531..2547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2531..2547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2544..2566)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2555..2571
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2579..2585,2586..2603,2604..2609))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2624..2645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2762..2779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2933..3521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3013..3032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3692..4483,4484..4552))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4315..4334
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4553..4657)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4705..4839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4742..4761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4796..4815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5007..5640
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5687..5812
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6309..6542
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6727..6771
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7034..7149
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7343..8525
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7574..8516
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8473..8493
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8580..8589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8586..8588,8589..8591,8592..9302)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8586..8588,8589..8591,8592..9302)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8631..8652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8892..8911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9239..9260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(10880..11124,1..330,331..333,334..342)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11047..11064)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
       61 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
      121 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
      181 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
      241 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
      301 tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga gacgaccttc
      361 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
      421 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
      481 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
      541 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
      601 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
      661 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
      721 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
      781 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
      841 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
      901 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta
      961 gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1021 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1081 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1141 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1201 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     1261 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     1321 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     1381 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     1441 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     1501 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     1561 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     1621 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     1681 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     1741 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     1801 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     1861 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     1921 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     1981 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     2041 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     2101 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     2161 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     2221 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     2281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     2341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     2401 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     2461 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg
     2521 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2581 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2641 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2701 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2761 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2821 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2881 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2941 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3001 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3061 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3121 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3181 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3241 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3301 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3361 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3421 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3481 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3541 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3601 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3661 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3721 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3781 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3841 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3901 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3961 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4021 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4081 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4141 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4201 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4261 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4321 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4381 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4441 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4501 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4561 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4621 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4681 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
     4741 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4801 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     4861 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     4921 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     4981 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     5041 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     5101 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     5161 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     5221 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     5281 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     5341 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     5401 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     5461 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5521 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5581 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5641 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     5701 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     5761 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     5821 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     5881 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     5941 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6001 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     6061 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     6121 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     6181 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     6241 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     6301 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     6361 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     6421 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     6481 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     6541 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     6601 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     6661 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     6721 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     6781 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     6841 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     6901 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     6961 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     7021 cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     7081 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     7141 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat gagtaattca
     7201 tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa ctcccactaa
     7261 cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc gtcatcactg
     7321 aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     7381 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     7441 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     7501 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     7561 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     7621 tggcccttgc gtgccttgaa ttacttccac gcccctggct gcagtacgtg attcttgatc
     7681 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     7741 cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg ccgccgcgtg cgaatctggt
     7801 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     7861 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     7921 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     7981 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     8041 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     8101 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     8161 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     8221 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     8281 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     8341 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     8401 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     8461 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     8521 cgtgaggatc tatttccggt gaattcctcg agactagttc tagagcggcc gcaccggtcg
     8581 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     8641 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     8701 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     8761 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     8821 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     8881 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     8941 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     9001 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     9061 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     9121 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     9181 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     9241 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     9301 agtactcaga tctcgacatg gcggtgtcca ggcggagggt tccccaggct ggagcgcggt
     9361 cgttcttctg cgccctgctg ctgtctttca gccaattcac cgggagcgac ggcacgggag
     9421 gcgacgctgc ggctcccggc gccgcgggca cccaggccga gttgccacac cgccgtttcg
     9481 agtacaaata cagctttaag gggccgcatc tggtgcagag cgatgggacc gtgccctttt
     9541 gggcccacgc ggggaatgct attccaagtg cagatcagat tcgaatagcg ccgtcattaa
     9601 aaagccaaag aggttccgtg tggaccaaag caaaagcagc cttcgagaac tgggaagtgg
     9661 aagtgacatt ccgagtgacc ggaagaggtc gaattggagc cgatggctta gcaatttggt
     9721 atacagaaaa tcaaggcttg gacggccctg tgtttggatc agctgatacg tggaatggtg
     9781 ttggaatatt ttttgattct tttgacaatg atggaaagaa aaataatcct gctatagtag
     9841 ttataggcaa caatggacaa atcaattatg accatcaaaa tgatggtgcc actcaagctt
     9901 tagcgagttg tcagagggac tttcgtaata aaccctatcc tgtccgggca aagattacgt
     9961 attaccagaa gacactgaca gtcatgatca ataatggctt tacaccagat aaaaatgatt
    10021 atgaattctg tgccaaagtg gaaaacatgg ttatccccac acaaggtcat tttgggatat
    10081 cagcggcaac agggggactt gcagatgacc acgacgttct ttctttcctg actttccaac
    10141 tgaccgagcc tggaaaagag ccacctacag cagaaaaaga catttcagaa aaagaaaagg
    10201 aaaagtatca agaggagttt gagcacttcc agcaagaatt agacaagaag aaggaggaat
    10261 tccagaaggg ccaccctgac ctccaggggc agccagcgga tgacatcttc gagagcatag
    10321 gcgatcggga gctgagacag gtgtttgaag gacagaatcg tattcatctg gagatcaagc
    10381 agctgaaccg gcagctggat atgattcttg atgaacagag gaggtacgtc tcgtccctga
    10441 cagaggagat ctccaggaga ggagcaggaa ccccgggaca gcctgggcag gtctctcagc
    10501 aggaactgga caccgttgtg aagagccagc aggagattct gagacaagtg aatgaggtga
    10561 agaactccat gagtgaaact gtgcggctgg tgagtggcat ccagcacccg ggctcagcag
    10621 gcgtctacga gacaactcag cacttcatgg acatcaagga acacctgcac gtggtgaaga
    10681 gagatatcga cagcctcgca cagcgcagca tgccatctaa tgaaaaacca aaatgcccag
    10741 acctaccacc attcccgtcg tgtctatcca cgatccactt tgtgatattt gtagtggtgc
    10801 agacagtgtt gttcgttggc tatatcatgt acaggactca gcaagaagca gctgccaaaa
    10861 aattcttttg aggatcccgc ccctctccct cccccccccc taacgttact ggccgaagcc
    10921 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
    10981 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
    11041 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
    11101 tggaagcttc ttgaagacaa acaa
//
