LOCUS       sequence-273539-        5643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5643)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-029   Vimentin-mVenus//BsaI
FEATURES             Location/Qualifiers
     source          1..5643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     primer_bind     complement(2559..2578)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    3027..3148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3064..3083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3118..3137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3949..3978
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4000..4587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4488..4507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4599..4626)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4650..5441,5442..5510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5273..5292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5511..5613)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5614..5643,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgagcaccag gagtgtcagt agctcctcct ataggcgtat gtttggcgga cctggaacag
      901 cttctcgacc cagttcatct cggtcatacg tcacgacttc aacacgaacc tatagcctgg
      961 gaagtgcgct taggcctagc actagtcgca gcttgtacgc atcttctcca ggtggggtgt
     1021 atgccacgcg ttctagtgcc gtgagactgc gttcttctgt tcctggcgta agactcctgc
     1081 aggattcagt cgacttcagc cttgcagacg ccatcaacac cgagttcaag aataccagga
     1141 ctaacgagaa ggtggagctg caggagctga atgatcggtt cgctaactac atcgataaag
     1201 tgagatttct cgagcagcag aacaagattc tgctggcaga gctggaacag ctgaaagggc
     1261 aaggcaaatc cagactcgga gacttgtacg aggaggaaat gcgggagctc aggcgacaag
     1321 tggatcagct gacaaacgac aaagctcgcg tggaagtcga gagagacaat ctggccgaag
     1381 atatcatgcg gcttcgggag aagttgcagg aagaaatgct gcagcgagag gaggcggaga
     1441 acacactcca gtcctttcgc caggatgtgg acaatgcttc cctggcacgg ctggacctcg
     1501 agaggaaagt tgaaagcctc caggaagaga tagcctttct caagaaactg cacgaagagg
     1561 aaatccagga actgcaagct cagattcagg agcaacacgt gcagattgat gtggacgtgt
     1621 ctaagcccga ccttaccgct gcactccgcg atgtccggca gcagtacgag tcagttgccg
     1681 ctaagaactt gcaagaagca gaggaatggt acaagagcaa gttcgctgac ctgagcgaag
     1741 cagcgaatcg caacaatgac gctcttagac aagccaagca ggagtctacg gagtatcgca
     1801 gacaggttca atccctgact tgcgaagttg atgccctgaa ggggaccaat gagagcttgg
     1861 aacgacaaat gcgtgagatg gaggaaaatt tcgccgtaga agctgccaac tatcaggaca
     1921 ctataggtcg cctgcaagac gagattcaga acatgaagga ggagatggcc aggcatctca
     1981 gggagtacca ggatctgctc aatgtcaaaa tggccctgga tatcgagatc gcaacttatc
     2041 ggaaactgct ggaaggcgag gagagtagaa tttcccttcc cttgccgaac ttctccagcc
     2101 tgaatcttag ggaaaccaac ctggactcac tgccactggt agacacacac tccaaaagga
     2161 ccctgttgat caagacagtg gaaaccagag atggccaggt gatcaacgaa acaagccagc
     2221 atcacgatga cctggagggc gggtcaggcg gcagcgtgtc aaagggagaa gagttgttta
     2281 ccggtgttgt tcccatactg gtggaactgg atggtgacgt caatggacac aagtttagcg
     2341 tttctggcga aggagaaggc gatgctacct atggcaaact gaccctcaaa ctgatctgta
     2401 cgactggaaa gttgcctgta ccttggccaa cactggtcac aaccttgggc tatgggctgc
     2461 aatgctttgc ccgttatcct gatcacatga agcaacatga cttctttaag tctgctatgc
     2521 cagagggata tgtgcaggaa cggactatct tcttcaagga cgacggcaac tacaagacga
     2581 gagctgaggt gaaatttgag ggtgacacac tggtgaatcg catagagctg aaaggcatcg
     2641 acttcaaaga agatgggaat attctcgggc ataaactcga gtacaactac aattcccaca
     2701 acgtgtacat cactgccgat aagcagaaga atgggattaa ggcgaacttc aagatccgac
     2761 acaacatcga ggatggtggg gtccagcttg ccgaccatta tcagcagaac acacccattg
     2821 gggatggacc agtcctgttg ccggataacc actacctcag ttaccagagc aaactgtcca
     2881 aagatcccaa tgagaagagg gaccatatgg tactgctcga attcgtgact gcagcaggca
     2941 ttacccttgg gatggatgaa ctttataagt caggctaata acagcttccg gactctagaa
     3001 catccctaca ggtgatatcc tcgggtaact tgtttattgc agcttataat ggttacaaat
     3061 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3121 gtttgtccaa actcatcaat gtatcttatc atgtctgtcg tcgaattcga gttggcgcgc
     3181 ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct
     3241 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
     3301 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
     3361 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
     3421 ggggctttgg gagggggctg tccccgtgag ctcccaggcg cgcctgtcat tctaaatctc
     3481 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     3541 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     3601 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     3661 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
     3721 ctgtccccgt gagctcccag gcgcgccaac tcggtacctc acccgattct catcatggag
     3781 acacatcagg acatctccaa taccgccatg gaaacgcacc aagatgcgac tgctcttctg
     3841 ccagtgacgg cattcgtctg caagattaac ggcatacaga aagaaacgat gagacccatt
     3901 caggacgagc ctcagactcc agcgtaactg gactgcaatc aactcactgg ctcaccttca
     3961 cgggtgggcc tttcttcggt agaaaatcaa aggatcttct tgagatcctt tttttctgcg
     4021 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4081 tcaagagcta ccaactcttt ttccgaggta actggcttca gcagagcgca gataccaaat
     4141 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4201 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4261 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4321 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4381 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4441 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4501 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcatcgatt tttgtgatgc
     4561 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cagaaaggcc cacccgaagg
     4621 tgagccaggt gattacattt gggccctcat taccaatgct taatcagtga ggcacctatc
     4681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4741 acgatgcggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
     4801 tcaccggctc cagatttatc agcaataaac cagccagccg ggagggccga gcgcagaagt
     4861 gatcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgcc
     5101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5161 actgtcatgc catccgtgag atgcttttct gtgactggtg agtactcaac caagtcattc
     5221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgtaaa
     5341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat tttagcttcc
     5521 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     5581 tggtgaaagt tggaacctct tacgtgccga tcaagtcaaa agcctccggt cggaggcttt
     5641 tga
//
