LOCUS       sequence-273540-        4317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4317)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-030   NLSNoLS-mVenus//BsaI
FEATURES             Location/Qualifiers
     source          1..4317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     CDS             843..890
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(1233..1252)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    1701..1822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1738..1757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1792..1811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2623..2652
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2674..3261
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3162..3181
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3273..3300)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(3324..4115,4116..4184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3947..3966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4185..4287)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(4288..4317,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggsgrggg gsggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgaaacggcc tgctgccacc aagaaagcag gacaggccaa gaagaagaaa cgcaggagaa
      901 ggcgacgtag aggcggtagc gggggcagcg tgtcaaaggg agaagagttg tttaccggtg
      961 ttgttcccat actggtggaa ctggatggtg acgtcaatgg acacaagttt agcgtttctg
     1021 gcgaaggaga aggcgatgct acctatggca aactgaccct caaactgatc tgtacgactg
     1081 gaaagttgcc tgtaccttgg ccaacactgg tcacaacctt gggctatggg ctgcaatgct
     1141 ttgcccgtta tcctgatcac atgaagcaac atgacttctt taagtctgct atgccagagg
     1201 gatatgtgca ggaacggact atcttcttca aggacgacgg caactacaag acgagagctg
     1261 aggtgaaatt tgagggtgac acactggtga atcgcataga gctgaaaggc atcgacttca
     1321 aagaagatgg gaatattctc gggcataaac tcgagtacaa ctacaattcc cacaacgtgt
     1381 acatcactgc cgataagcag aagaatggga ttaaggcgaa cttcaagatc cgacacaaca
     1441 tcgaggatgg tggggtccag cttgccgacc attatcagca gaacacaccc attggggatg
     1501 gaccagtcct gttgccggat aaccactacc tcagttacca gagcaaactg tccaaagatc
     1561 ccaatgagaa gagggaccat atggtactgc tcgaattcgt gactgcagca ggcattaccc
     1621 ttgggatgga tgaactttat aagtcaggct aataacagct tccggactct agaacatccc
     1681 tacaggtgat atcctcgggt aacttgttta ttgcagctta taatggttac aaataaagca
     1741 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1801 ccaaactcat caatgtatct tatcatgtct gtcgtcgaat tcgagttggc gcgcctgtca
     1861 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     1921 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     1981 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     2041 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     2101 ttgggagggg gctgtccccg tgagctccca ggcgcgcctg tcattctaaa tctctctttc
     2161 agcctaaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa
     2221 gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct
     2281 gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc
     2341 tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg ggggctgtcc
     2401 ccgtgagctc ccaggcgcgc caactcggta cctcacccga ttctcatcat ggagacacat
     2461 caggacatct ccaataccgc catggaaacg caccaagatg cgactgctct tctgccagtg
     2521 acggcattcg tctgcaagat taacggcata cagaaagaaa cgatgagacc cattcaggac
     2581 gagcctcaga ctccagcgta actggactgc aatcaactca ctggctcacc ttcacgggtg
     2641 ggcctttctt cggtagaaaa tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2701 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2761 gctaccaact ctttttccga ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2821 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2881 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2941 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3001 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3061 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3181 tatagtcctg tcgggtttcg ccacctctga cttgagcatc gatttttgtg atgctcgtca
     3241 ggggggcgga gcctatggaa aaacgccagc aacgcagaaa ggcccacccg aaggtgagcc
     3301 aggtgattac atttgggccc tcattaccaa tgcttaatca gtgaggcacc tatctcagcg
     3361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgatg
     3421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg
     3481 gctccagatt tatcagcaat aaaccagcca gccgggaggg ccgagcgcag aagtgatcct
     3541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgccagaagt
     3781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3841 atgccatccg tgagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg taaactctca
     4021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcattttagc ttccttagct
     4201 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     4261 aagttggaac ctcttacgtg ccgatcaagt caaaagcctc cggtcggagg cttttga
//
