LOCUS       sequence-273541-       10256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged recycling endosome marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 10256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..10256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             73..706
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    753..878
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1375..1608
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1793..1837
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2100..2215
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2409..3591
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2640..3582
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3539..3559
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3635..3644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3641..3643,3644..3646,3647..4357)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3641..3643,3644..3646,3647..4357)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3686..3707)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3947..3966)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4294..4315
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5078..5652,5653..5655,5656..5664)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5245..5262)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5472..5491
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5684..6283
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5684..5703)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6180..6200
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6297..6885
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6350..6370)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6768..6779)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7092..7725
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7853..7869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7853..7869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7866..7888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7877..7893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7901..7907,7908..7925,7926..7931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7946..7967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8084..8101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8255..8843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8335..8354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9014..9805,9806..9874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9637..9656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9875..9979)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10027..10161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10064..10083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10118..10137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
       61 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
      121 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
      181 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
      241 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
      301 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
      361 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
      421 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
      481 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
      541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      721 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      781 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      841 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      901 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      961 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1021 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1081 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1141 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1201 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1261 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1321 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1381 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1441 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1501 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1561 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1621 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1681 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1741 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1801 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1861 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1921 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1981 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2041 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct
     2101 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2161 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2221 tttattacag ggacagcaga gatccagttt atcgatgagt aattcataca aaaggactcg
     2281 cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta gaacccagag
     2341 atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt ggagaagagc
     2401 atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2701 cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     2761 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     2821 gttgaggcct ggcttgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     2881 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     2941 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     3001 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     3061 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     3121 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     3181 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     3241 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     3301 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     3361 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     3421 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     3481 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     3541 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggatctatt
     3601 tccggtgaat tcctcgagac tagcgctacc ggtcgccacc atggtgagca agggcgagga
     3661 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3721 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3781 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3841 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3901 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3961 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4021 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4081 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4141 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4201 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4261 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4321 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagccat
     4381 ggggacccgg gacgacgagt acgactacct attcaaagtg gtgctcatcg gggactcagg
     4441 cgtgggcaag agcaacctgc tgtcgcgctt cacccgcaac gagttcaacc tggagagcaa
     4501 gagcaccatc ggcgtggagt tcgccacccg cagcatccag gtggacggca agaccatcaa
     4561 ggcgcagatc tgggacaccg ctggccagga gcgctaccgc gccatcacct ccgcgtacta
     4621 ccgtggtgca gtgggcgccc tgctggtgta cgacatcgcc aagcacctga cctatgagaa
     4681 cgtggagcgc tggctgaagg agctgcggga ccacgcagac agcaacatcg tcatcatgct
     4741 ggtgggcaac aagagtgacc tgcgccacct gcgggctgtg cccactgacg aggcccgcgc
     4801 cttcgcagaa aagaacaact tgtccttcat cgagacctca gccttggatt ccactaacgt
     4861 agaggaagca ttcaagaaca tcctcacaga gatctaccgc atcgtgtcac agaaacagat
     4921 cgcagaccgc gctgcccacg acgagtcccc ggggaacaac gtggtggaca tcagcgtgcc
     4981 gcccaccacg gacggacaga agcccaacaa gctgcagtgc tgccagaacc tgtgataaga
     5041 attctgcagt cgacggtacc gcgggcccgg gatcccgccc ctctccctcc ccccccccta
     5101 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     5161 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     5221 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5281 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5341 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     5401 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5461 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     5521 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     5581 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     5641 cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc
     5701 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     5761 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     5821 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5881 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5941 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6001 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6061 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6121 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6181 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6241 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tagacgcgtc tggaacaatc
     6301 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6361 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6421 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6481 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6541 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6601 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6661 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     6721 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6781 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6841 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     6901 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     6961 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     7021 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7081 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     7141 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7201 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     7261 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     7321 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     7381 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     7441 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     7501 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     7561 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7621 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7681 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7741 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     7801 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7861 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7921 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7981 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8041 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8101 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8701 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8881 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8941 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9001 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9061 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9121 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9181 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9241 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9301 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9361 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9421 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9481 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9541 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9601 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9661 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9721 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9781 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9841 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9901 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9961 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    10021 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10141 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    10201 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaac
//
