LOCUS       sequence-273554-       14436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to Sox17 gene locus for induction of P2A-fused 
            phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by 
            FRT(F5) sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata 
            S, Ikawa M, Okano H
  TITLE     Developmental analyses of mouse embryos and adults using a 
            non-overlapping tracing system for all three germ layers.
  JOURNAL   Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 
            10.1242/dev.174938.
  PUBMED    31597657
REFERENCE   2  (bases 1 to 14436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2019-11-4"; volume: "146(21). pii: 
            dev.174938. doi"; pages: " 10.1242/dev.174938"
COMMENT     Sequence Label: Sox17(2A-phiC31o)
FEATURES             Location/Qualifiers
     source          1..14436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5898..5954
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5958..5978
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5979..7793
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             7794..7814
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7862..7910
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7917..8516)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8000..8020)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8497..8516
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8535..8582)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(8594..9093)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8642..8661)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9035..9058
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(12053..12069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12053..12069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12066..12088)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12077..12093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12101..12107,12108..12125,12126..12131))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12146..12167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12226..12421
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12272..12407
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12427..12561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12464..12483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12518..12537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12629..12646)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12800..13388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12880..12899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13559..14350,14351..14419))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14182..14201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accggttact gagctgggaa gtggagcggg agcagtttac ttcctgcttc gccgcggaaa
       61 aactcagcta gggccgagcg ggaaggagga cgtggtctcc gagtcgccag ctgctagggt
      121 ggggtttcgg ggcacgatta gacagagatt cggacagtta cgtgagaccc cgcccaccca
      181 ggatgcacgc tcccaacggt gtgcggaatg agcccccgaa gttaatcttg gggtcagacg
      241 ttctcagctc tcacgggaga ggctagcaaa gcgaagattg cagggtctgg cctgaatgtt
      301 ctgggccacc gtgggggccg gtgatcactg tctttcttgc cactctttcc tctcaagttc
      361 ccgaatttcc atctccacct ccgacccact ggggactgac cagggactgg aaagagcgcg
      421 aaaggtataa aggggagaag agactgcgcc ttttggtgcc cgggtgaaag gtgccaatcg
      481 accgcatcgc tctgcgtggc cctacctggg aggtgcggag cccggggcac gacgctcggt
      541 gttctcagag gcagactctg aggcacgtat tttagaccgt ccagtggtac tattgtctct
      601 gttatcttag agtccgccgg gggctgtctc cgtgatgaca gcccacgctc ccttttccgc
      661 cctcatgaac ttgctctcca cagaccacgg atctgcttga gtgcccacgg atcctgtgca
      721 attggacttg aatgtccttt aacaggcctt ggccatcaga acaccaaaac cataaaacct
      781 tcgagaaatc aaccactcac tctgaggctc gctgtagaag agtggcttag actctcccac
      841 ttcggagtcc cagttgatca ccaacccgct tgctacaggc cccaaatggt tacgggcaca
      901 gcttctagtt acattagacg cttccttgcc ccagcctttc gagcctcagg tctgctttgg
      961 ggatgtggct taggcagtag ccacctagaa atcctgcact gtttgagtgt gataccgctg
     1021 cggactacag cagacaggcg tgccaggctt ctagtccaga tacccacccc caccccagta
     1081 aatctctagt gttgagggaa gcaaggcttg caggaagccg ctccggatgt ttgcttcttt
     1141 acttgtggca tttgtggctg gctttgataa cgtcgtgagc tcggcttcca acacccattt
     1201 agtgaagaaa ctgaaatatg gcccactcac actgctggcg ggtctgaagt gcggttggcc
     1261 ccaacactcc tcccaaagta tctatcaaga gaatggtcag cagaagttag atctaggtaa
     1321 gaggacggga agggggacta gtcttgggaa agcgccacat ttcctggtag atttgttaag
     1381 taaagtgcta tctagggact ccaggggagg ctgttttaag gaacggggaa gagcccaaaa
     1441 gtggtcgctt ggactcctac tgtgtttgtg ctttccctca gaaagatcag ttgttaatct
     1501 tcagggatgc ttgtaaataa cgctgcctcc ccatccccgg gctttctttc taaccacttt
     1561 ctcagttcct tttatggagc tcgaacagaa ctaggtaccc aggtaaaatg gtgtaaatac
     1621 attttctaga cctacacctg aattatattt ctgttgtctc ttagttttta gagaaatcaa
     1681 ccaagacagg gaagacagag aaaatgcatt ctggacccgc tactgttttc aatcgtcatt
     1741 tcaaacaaaa cccgtagttt atttaaaaaa aaaaaaaaca cggaaagaga aaattaggcc
     1801 ctgagatctt tgaaaagatg cccattacta gatacaaaag agaagagtca ctgtggaggt
     1861 gagggactgc gtccctgctt ctgagaggca ggagagctac atggggagac tgtctcaaaa
     1921 ctaacaacag aagagataga attttttttt tcttaaagag cacctggcta gagaatcgat
     1981 ttgtaaaatt gctatttcta cccttagagt ttccttgatt aaacatctga aatgcatttt
     2041 ttttgtttta ctttcaagct aatgtttttc agaaaaaaat aaaaagcttt gaggaaaagc
     2101 acattatcag ttagatttaa gaccaattgc ttttaaatta ccaatttaaa atatcatttt
     2161 ttttatttct taaagcagat ttaattttac cccctttgta tgaatcttac agacctggag
     2221 ttttaactac tatcactgat ctctacacac aacctccagc tttaaatggg agggagggtc
     2281 accactgctt tataacataa caaattagag aaaagctggc ttctgtatga gttctttgga
     2341 gacaaagtag ctccagaaac tgcagaccag aagctatcaa aatctctgtg cttttttgga
     2401 acctccagta agccagattt ggtctctgaa ataatacagg aaaacctcag catgtcacct
     2461 catggataca agtaagtaat acttttcaat cagctattca cagtaaagaa agtctgtttt
     2521 ggaaactgac atgttgagtc cttccttcct tccttccttc cttccttcct tccttccttc
     2581 cttccttcct ccctctctcc ctccctccct tccttccttt cttcctttct tcctctcctt
     2641 caagacaggg ttgcatgtag cccaggctgg cctctaattc catatgtagc taaggacaat
     2701 catgaacgta tttttctgcc ccccacatct caagtgctgg aattatagaa ttacaggcac
     2761 taggcatctc atagactgtc tttcggaatt gtactagatg aacattcaaa ctgagctcga
     2821 tcaccattcc tttgagtttt cctaccttaa aactacgcaa ggatgaccgt tggatctggt
     2881 agagaacaac acaaacatgc aaacaaactc aaatgtaaca cagagctttt ggagctcctt
     2941 gtctgaagga gggctgctaa gtgtgtgtaa cactctccca gtgtctgcct taacaggtga
     3001 tgagtctccc tttcctctcc tagaatctat aatgctgccc ttgaaaaaga ctacgtctag
     3061 aactaaatga gtttgctaat atttggaggg tgctgagtgg ttgctgggat aagcattgta
     3121 ggaaggcatg ttttgaagcc cgtgggaaag tactttcagg tttaaaataa atttaagggt
     3181 ggtcagcttt tcattccagt cttaagaaaa cgttgtaacc gtctgtcagg ctaacagtct
     3241 tacacgctac ggatttgctg cttaaatgga ctgatccttt tcctccgagg aacagcacag
     3301 tgctgctgtc tttgcacttt attttacatt ttaatctcgt ggtgtttgcg atggaatcat
     3361 ggccttgcaa gtctttagaa ataaaaccag atcttgcctc ttttgccgaa cacacaaaag
     3421 gagcttttgt tttatatttt tatgtttcac actgaaattc ctcagggttg acttgagctc
     3481 ttattgagca gtgatatatt agtgatatag cattttatgt taaactttgt tttattacaa
     3541 cttgctattt accttggata tttttattat aatataagca tgatggatga gttcttggga
     3601 gaagaacagg attttttttc tctgaaaaat catgctaagt taattacctt cggtaatatt
     3661 tttttaaaag aatggaataa atacctatac ctagtatgtt gatgtgaaca accttggact
     3721 tgttcttcaa tcttccgtta cagtgagcag cacctccaga catctgaatt tcagccttcc
     3781 tatttcccca agaggtcttg gcgccagcgc ccggctccag ccagttttcc ccaaggcagg
     3841 tgtagccaga acactgccgg gggaggggaa aggggcgggt gtgggacttg ggactcggac
     3901 cactggctgg gcgggggcct aaggactagg gtccggtccg cccccgcctc gccattggcc
     3961 gcgtccataa agaaaagggc cccgcggccc ggggggacac ccgcagtgtc actgggtaga
     4021 tctccagcgc ggattaggcg aagggttagg tctaccagga gagcagcggg agggtagcac
     4081 ccgctcagct ttacgagttc ctctgggcat ctccaggcta gcttccgatc cctgcctcag
     4141 ggtcggggga agcggcgtgt cccgtggcca tagcagagct cggggtcggt ctggagagcc
     4201 atgagcagcc cggatgcggg atacgccagt gacgaccaga gccagccccg gagcgcgcag
     4261 cccgcggtga tggcagggtt gggcccctgt ccctgggccg agtccctgag ccccctcggg
     4321 gatgtaaagg tgaaaggcga ggtggtggcg agtagcgggg cgccagccgg gacgtcgggc
     4381 cgagccaaag cggagtctcg catccggcgg ccgatgaacg cctttatggt gtgggccaaa
     4441 gacgaacgca agcggttggc acagcagaac ccagatctgc acaacgcaga gctaagcaag
     4501 atgctaggtg agtcggacca gggggataca gggcaccggc gcactccaga gaatctcctt
     4561 gagccacttc cttgagccaa gaggtgtgct gtagactcaa tcccttcttc cagctccctc
     4621 tctcgccaaa gtaacacttt ggtctttggg ccataggagc gaatgtaagg gtttctcagt
     4681 atttcagaga ggtaacccgc acagccctgg ctcgacccct tcccagatca ggggactttg
     4741 gcagtttttc gagttaaggg gagaaaggta agggaggaag ccccttctag gcgtcctgta
     4801 aggacgcgat ctaacacttt catccgaata ggaccgggct tctcacctgc tcgctcagtg
     4861 agaggttgca ccagagaggg gtcagggtgc cggagctcac tcgggtaacc ctgcgcgcct
     4921 ctccaccttt ccctgcaggc aagtcttgga aggcgttgac cttggcagag aagcggccct
     4981 tcgtggaaga ggccgagcgg ctgcgcgtgc agcatatgca ggaccacccc aactacaagt
     5041 accggccgcg gcggcgcaag caggtgaagc gcatgaagcg ggtggaggga ggcttcctgc
     5101 acgctctcgt cgagccccag gccggcgcgc ttggtcccga gggcggccgc gtggccatgg
     5161 atggcctggg tctgcctttc ccggagccgg gctatccggc cggtcctccg ctgatgtctc
     5221 cgcacatggg cccccactat cgggactgcc agggactggg cgctcccgcg ctcgacggct
     5281 accctctgcc cactccggac acatccccgc tggatggcgt ggagcaggac ccggctttct
     5341 ttgcagcccc gctgccaggg gactgcccgg cggccggcac ctacacttac gctccagtct
     5401 cggactatgc agtgtccgta gagccgcccg ctggccccat gcgagtgggg ccggacccct
     5461 cgggccctgc gatgccgggg atcctggcgc cccccagcgc tctgcacctg tactacggcg
     5521 cgatgggctc gcccgccgca agtgcggggc gcggtttcca cgcgcaaccc cagcagccgc
     5581 tgcaaccgca ggcaccgccg ccgccaccgc agcagcagca cccagcgcac ggccccgggc
     5641 aaccttcgcc ccctcccgag gctctgccct gccgggatgg cacggaatcc aaccagccca
     5701 ctgagctcct aggggaggtg gaccgcacgg aattcgaaca gtatctgccc tttgtgtata
     5761 agcccgagat gggtcttccc taccagggac acgactgcgg agtgaacctc tcagacagcc
     5821 acggagccat ttcctccgtg gtgtccgacg ctagctcagc ggtctactat tgcaactacc
     5881 ccgacattgg aagcggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg
     5941 agaaccctgg acctgatcca aaaaagaaga gaaaggtaat ggatacctac gccggagcct
     6001 acgacagaca gagccgggag agagagaaca gcagcgccgc cagccccgcc acccagagaa
     6061 gcgccaacga ggataaggcc gccgatctgc agagagaggt ggagagggac ggcggcagat
     6121 tcagatttgt gggccacttc agcgaggccc ctggcaccag cgccttcggc accgccgaga
     6181 gacccgagtt cgagagaatc ctgaacgagt gtagggccgg caggctgaac atgatcatcg
     6241 tgtacgacgt gtcccggttc agcaggctga aggtgatgga cgccatccct atcgtgtccg
     6301 agctgctggc cctgggcgtg accatcgtgt ccacccagga aggcgtcttt agacagggca
     6361 acgtgatgga cctgatccac ctgatcatga ggctggacgc cagccacaag gagagcagcc
     6421 tgaagagcgc caagatcctg gacaccaaga acctgcagag ggagctgggc ggctatgtgg
     6481 gcggcaaggc cccctacggc ttcgagctgg tgtccgagac caaggagatc acccggaacg
     6541 gcaggatggt gaacgtggtg atcaacaagc tggcccacag caccaccccc ctgaccggcc
     6601 ccttcgagtt tgagcccgac gtgatcaggt ggtggtggcg ggagatcaag acccacaagc
     6661 acctgccttt caagcccggc agccaggccg ccatccaccc cggcagcatc accggcctgt
     6721 gtaagagaat ggacgccgac gccgtgccca ccagaggcga gaccatcggc aagaaaaccg
     6781 ccagcagcgc ctgggacccc gccaccgtga tgagaatcct gagggaccct aggatcgccg
     6841 gcttcgccgc cgaggtgatc tacaagaaga agcccgacgg cacccccacc accaagatcg
     6901 agggctacag aatccagaga gaccccatca ccctgagacc tgtggagctg gactgtggcc
     6961 ctatcatcga gcctgccgag tggtacgagc tgcaggcctg gctggacggc agaggcagag
     7021 gcaagggcct gagcagaggc caggccatcc tgagcgccat ggacaagctg tactgtgagt
     7081 gtggcgccgt gatgaccagc aagagaggcg aggagagcat caaggacagc taccggtgcc
     7141 ggagaagaaa ggtggtggac cccagcgccc ctggccagca cgagggcacc tgtaatgtga
     7201 gcatggccgc cctggacaag ttcgtggccg agcggatctt caacaagatc cggcacgccg
     7261 agggcgacga ggagaccctg gccctgctgt gggaggccgc cagaagattc ggcaagctga
     7321 ccgaggcccc cgagaagagc ggcgagaggg ccaacctggt ggccgagaga gccgacgccc
     7381 tgaacgccct ggaggagctg tacgaggaca gagccgccgg agcctatgac ggccctgtgg
     7441 gcaggaagca cttcagaaag cagcaggccg ccctgaccct gagacagcag ggcgccgagg
     7501 aaagactggc cgagctggag gccgccgagg cccctaagct gcccctggat cagtggttcc
     7561 ccgaggatgc cgacgccgac cccaccggcc ccaagtcctg gtggggcaga gccagcgtgg
     7621 acgacaagag ggtgttcgtg ggcctgttcg tggataagat cgtggtgacc aagagcacca
     7681 ccggcagggg ccagggcacc cccatcgaga agagagccag catcacctgg gccaagcctc
     7741 ccaccgacga cgacgaggat gacgcccagg acggcaccga ggacgtggcc gcccctaaga
     7801 aaaagcggaa agtgtgatag atgaagttcc tatacctttt gaagaatagg aacttcgatc
     7861 cgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg atcccctcag
     7921 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
     7981 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
     8041 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
     8101 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
     8161 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
     8221 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     8281 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     8341 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     8401 ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     8461 gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggtt
     8521 tagttcctca ccttgtcgta ttatactatg ccgatatact atgccgatga ttaattgtca
     8581 acaggctgca ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc
     8641 gcttttgaag cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc
     8701 tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag
     8761 cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag
     8821 cggtgctgtc catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc
     8881 acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg
     8941 cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc
     9001 gaggccagag gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc
     9061 cagactgcct tgggaaaagc gcctccccta cccggtagaa ttgacctgca ggggccctcg
     9121 atagcttggc tggacgtaaa ctcctcttca gacctgaagt tcctatacct tttgaagaat
     9181 aggaacttcc ggttgccgac ccgacctgag ggccagaagc agtgttacac acttcctgga
     9241 agcgctaagg aaatccttag cgtcaagctg ttttgttttt aagttgcctt ggcatataat
     9301 ttatggtaat ttattttgtc tgccacttga acagttgagg ggctacacga gatttttgtt
     9361 ttaaatgcct tgagggactt gctccccaca atcactgtcc agttttagcc agttttaaaa
     9421 tatctcactg acttattcca tttcttgaaa gtgtattgat ctagagaaat tttgtcatgc
     9481 gtgttttctc aatcttaaat aaatcctgga atctttttaa aatctctcgc atcgattttt
     9541 atcaaattaa agatcaacag taagtattat acaattagaa gtgtatactt aattgtaaaa
     9601 ttttcatttt tcttacaatg gtaagaaaat gcatctaaga aaccctaaac acaaacagcg
     9661 gggtgttcaa atgtgatata attgttctga gaaaaattct caaaagatga acttgttatg
     9721 tacattccaa atattggttt ctaaaaatga tactgtaaat gtttttaatt ctgtacactt
     9781 taatgaggct gttcaaaaat ttcggatttt cagtcatctc gacacctgcc aaaagagctt
     9841 ttcagatata actctttcct gtttgtcttt tcttccaaaa gtcttgatcc cacttcccac
     9901 aaaatttgcc tttaattcct acaattttag agtcagagaa actaatcaaa ataaagtttg
     9961 tgttgtgact gttagtgaac tttagtatgt aatttcaacc gtgcatcttg ctatagctag
    10021 attgtgtccc ttcattttac agttttcagg ctcaagattc tcctagagtg atttgtgtgt
    10081 ggtgtgcttt acgcaggtga agacacaaac cagcatccgt cctattgtga agaaacaggt
    10141 acaagactag cccctgcaaa tgctgtgctg agtgctgcgg tccacatggc catcatagga
    10201 actgctggtt tgtgggcttt tcctgtgcca ggtgtttcct tagtgtcatt ctgcacagct
    10261 tcggtctgag caagcctgcc tcccagttga gcgctattct ctcattttcc agtccccata
    10321 caggctgggg actgaagcca cagcttcatc aagggagaca agagaaccac ctttgagctg
    10381 caaacccagt ccagatgcta actcctaatg tatttacaga gtctctttgt gtagggggca
    10441 taggtgggca ctagaattca cattctggtc aacatgtaag gtatttagat gatggaaaac
    10501 ttgaaggcga tggatttgaa gggttccatg gaaatgtcag tgtaacattt taaagggggg
    10561 attcctgatt actaggcaca gaagggtaga cattttcagt gacaaaaggt tgagcctact
    10621 ttgttggaaa tttctagtga ctataatcat tttaaaattt ctatttaaaa atttaaagtt
    10681 catcccaggg agtttatcta acctatttcc ttttcctttc tccctcctag tccctgacac
    10741 taggcaggaa aaccagagtc tctacaacct gtctcatttt attattactt tttgttgttg
    10801 ttgttgttgt tgttttctag ctgtcctgga actcactttg tagaccaggc tggcttcaaa
    10861 cccacagaga tctgcatacc tctgcctcct aagtactgtg actaaaggca tgtaccaaga
    10921 catcaggctt ccaggctgat gtttatttag tgctcttccc tgctctacac tgtttctttc
    10981 atcccagaga ggttgttctg cttgagcata agttcattta cagggatttc caagacgttc
    11041 tcagtagact atgtaatgag cagaggattt gtccaggctc accttcaagg ccacatggct
    11101 agttgaaatt tctctagaca ttttattgtg tgaatccaaa gaaacaactg ctggaaattt
    11161 aactcttaaa ataaagaagg taagtaggga aaggggaaag agagaaagaa tctctaatac
    11221 ttatggcaaa gtccaaaatc agattacatt acattttttt ttttttagag tgagcaattt
    11281 ttattgaaac cctcaaagat taaagaggac caaaagatga gttcggtacc ataacaaaaa
    11341 aaatctcacc tacattacat ttttaaccac tcctaaggtg tgaataggaa gacaagacag
    11401 ctaaaattat tgaggcaatg ttactataca gatggaggtc tcttcatcct cacagacaac
    11461 tcttgtaggt agagactttg ggctagttcc tttcctcaag cactcaggta aagtgatcaa
    11521 agaatctgct aatggttgtt ttgtcctttg atatcatctc atttttcttt cacccttgta
    11581 tggagaaaaa acagccaata aaggacacca tgctccctac tgcgtctttc tgagatcctt
    11641 tagaaataga tatgagcatc tagggcaggt ttctgttggg ttctaagcag ctttgtatgt
    11701 ttgcatattt gccttctcct tatgattggc tgagaaaaac aggaattctt ctacaggaat
    11761 cctagtaaca cacatacaaa gcacacactc tttaattctg gcattatgtg actctttcca
    11821 gctatcctcg ggttacagaa gcctcggtgt tggtcagcaa ataacacaga cagttcaagc
    11881 atccaaaaca tgacaggcat cagatatttg aaatcaaaga actatttctt cgaggaattc
    11941 tttgttatga gtcacatgat tcacatgatt taactaacag atactattct cttgaattta
    12001 aagggagcct ataatgtatg gtgagatgaa gatgaagctt ggcgtaatca tggtcatagc
    12061 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    12121 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    12181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
    12241 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    12301 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    12361 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    12421 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    12481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    12541 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    12601 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    12661 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    12721 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    12781 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    12841 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    12901 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    12961 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    13021 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    13081 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    13141 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    13201 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    13261 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    13321 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    13381 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    13441 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    13501 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    13561 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    13621 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    13681 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    13741 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    13801 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    13861 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    13921 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    13981 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    14041 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    14101 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    14161 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    14221 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    14281 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    14341 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    14401 ggaaatgttg aatactcata ctcttccttt ttcaat
//
