LOCUS       sequence-273575-       10861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Himar-dCas9 gene with C-terminal 6x-His tag for protein 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Chen SP, Wang HH
  TITLE     An Engineered Cas-Transposon System for Programmable and 
            Site-Directed DNA Transpositions.
  JOURNAL   CRISPR J. 2019 Dec;2(6):376-394. doi: 10.1089/crispr.2019.0030. Epub
            2019 Nov 19.
  PUBMED    31742433
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2019.0030"; journalName: "CRISPR J"; date: 
            "2019-12"; volume: "2"; issue: "6"; pages: "376-394"
COMMENT     Sequence Label: pET-Himar-dCas9
FEATURES             Location/Qualifiers
     source          1..10861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..125
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     209..228
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        209..227
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    228..252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             267..289
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(1388..1414,1415..1417,1418..3904,3905..3907,
                     3908..5491)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5492..5509
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5562..5580)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5576..5623
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(5861..5879)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5947..6051
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6052..6120,6121..6912)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6270..6289)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7083..7671
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7572..7591
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7825..7842
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7857..7996
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7922..7941)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8082..8104
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8098..8289)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(9601..10683)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10645..10664
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10684..10761)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
      241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat ataccatgga
      301 aaaaaaggaa tttcgtgttt tgataaaata ctgttttctg aagggaaaaa atacagtgga
      361 agcaaaaact tggcttgata atgagtttcc ggactctgcc ccagggaaat caacaataat
      421 tgattggtat gcaaaattca agcgtggtga aatgagcacg gaggacggtg aacgcagtgg
      481 acgcccgaaa gaggtggtta ccgacgaaaa catcaaaaaa atccacaaaa tgattttgaa
      541 tgaccgtaaa atgaagttga tcgagatagc agaggcctta aagatatcaa aggaacgtgt
      601 tggtcatatc attcatcaat atttggatat gcggaagctc tgtgcgaaat gggtgccgcg
      661 cgagctcaca tttgaccaaa aacaacgacg tgttgatgat tctaagcggt gtttgcagct
      721 gttaactcgt aatacacccg agtttttccg tcgatatgtg acaatggatg aaacatggct
      781 ccatcactac actcctgagt ccaatcgaca gtcggctgag tggacagcga ccggtgaacc
      841 gtctccgaag cgtggaaaga ctcaaaagtc cgctggcaaa gtaatggcct ctgttttttg
      901 ggatgcgcat ggaataattt ttatcgatta tcttgagaag ggaaaaacca tcaacagtga
      961 ctattatatg gcgttattgg agcgtttgaa ggtcgaaatc gcggcaaaac ggccccacat
     1021 gaagaagaaa aaagtgttgt tccaccaaga caacgcaccg tgccacaagt cattgagaac
     1081 gatggcaaaa attcatgaat tgggcttcga attgcttccc cacccgccgt attctccaga
     1141 tctggccccc agcgactttt tcttgttctc agacctcaaa aggatgctcg cagggaaaaa
     1201 atttggctgc aatgaagagg tgatcgccga aactgaggcc tattttgagg caaaaccgaa
     1261 ggagtactac caaaatggta tcaaaaaatt ggaaggtcgt tataatcgtt gtatcgctct
     1321 tgaagggaac tatgttgaaa gcggttccga aactcccggt acatcagaaa gcgcgacccc
     1381 cgaaagcatg gataaaaagt attctattgg tttagctatc ggcacaaata gcgtcggatg
     1441 ggcggtgatc actgatgaat ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac
     1501 agaccgccac agtatcaaaa aaaatcttat aggggctctt ttatttgaca gtggagagac
     1561 agcggaagcg actcgtctca aacggacagc tcgtagaagg tatacacgtc ggaagaatcg
     1621 tatttgttat ctacaggaga ttttttcaaa tgagatggcg aaagtagatg atagtttctt
     1681 tcatcgactt gaagagtctt ttttggtgga agaagacaag aagcatgaac gtcatcctat
     1741 ttttggaaat atagtagatg aagttgctta tcatgagaaa tatccaacta tctatcatct
     1801 gcgaaaaaaa ttggtagatt ctactgataa agcggatttg cgcttaatct atttggcctt
     1861 agcgcatatg attaagtttc gtggtcattt tttgattgag ggagatttaa atcctgataa
     1921 tagtgatgtg gacaaactat ttatccagtt ggtacaaacc tacaatcaat tatttgaaga
     1981 aaaccctatt aacgcaagtg gagtagatgc taaagcgatt ctttctgcac gattgagtaa
     2041 atcaagacga ttagaaaatc tcattgctca gctccccggt gagaagaaaa atggcttatt
     2101 tgggaatctc attgctttgt cattgggttt gacccctaat tttaaatcaa attttgattt
     2161 ggcagaagat gctaaattac agctttcaaa agatacttac gatgatgatt tagataattt
     2221 attggcgcaa attggagatc aatatgctga tttgtttttg gcagctaaga atttatcaga
     2281 tgctatttta ctttcagata tcctaagagt aaatactgaa ataactaagg ctcccctatc
     2341 agcatcaatg attaaacgct acgatgaaca tcatcaagac ttgactcttt taaaagcttt
     2401 agttcgacaa caacttccag aaaagtataa agaaatcttt tttgatcaat caaaaaacgg
     2461 atatgcaggt tatattgatg ggggagctag ccaagaagaa ttttataaat ttatcaaacc
     2521 aattttagaa aaaatggatg gtactgagga attattggtg aaactaaatc gtgaagattt
     2581 gctgcgcaag caacggacct ttgacaacgg ctctattccc catcaaattc acttgggtga
     2641 gctgcatgct attttgagaa gacaagaaga cttttatcca tttttaaaag acaatcgtga
     2701 gaagattgaa aaaatcttga cttttcgaat tccttattat gttggtccat tggcgcgtgg
     2761 caatagtcgt tttgcatgga tgactcggaa gtctgaagaa acaattaccc catggaattt
     2821 tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt attgaacgca tgacaaactt
     2881 tgataaaaat cttccaaatg aaaaagtact accaaaacat agtttgcttt atgagtattt
     2941 tacggtttat aacgaattga caaaggtcaa atatgttact gaaggaatgc gaaaaccagc
     3001 atttctttca ggtgaacaga agaaagccat tgttgattta ctcttcaaaa caaatcgaaa
     3061 agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt
     3121 tgaaatttca ggagttgaag atagatttaa tgcttcatta ggtacctacc atgatttgct
     3181 aaaaattatt aaagataaag attttttgga taatgaagaa aatgaagata tcttagagga
     3241 tattgtttta acattgacct tatttgaaga tagggagatg attgaggaaa gacttaaaac
     3301 atatgctcac ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc gttatactgg
     3361 ttggggacgt ttgtctcgaa aattgattaa tggtattagg gataagcaat ctggcaaaac
     3421 aatattagat tttttgaaat cagatggttt tgccaatcgc aattttatgc agctgatcca
     3481 tgatgatagt ttgacattta aagaagacat tcaaaaagca caagtgtctg gacaaggcga
     3541 tagtttacat gaacatattg caaatttagc tggtagccct gctattaaaa aaggtatttt
     3601 acagactgta aaagttgttg atgaattggt caaagtaatg gggcggcata agccagaaaa
     3661 tatcgttatt gaaatggcac gtgaaaatca gacaactcaa aagggccaga aaaattcgcg
     3721 agagcgtatg aaacgaatcg aagaaggtat caaagaatta ggaagtcaga ttcttaaaga
     3781 gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc tatctctatt atctccaaaa
     3841 tggaagagac atgtatgtgg accaagaatt agatattaat cgtttaagtg attatgatgt
     3901 cgatgccatt gttccacaaa gtttccttaa agacgattca atagacaata aggtcttaac
     3961 gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa
     4021 gatgaaaaac tattggagac aacttctaaa cgccaagtta atcactcaac gtaagtttga
     4081 taatttaacg aaagctgaac gtggaggttt gagtgaactt gataaagctg gttttatcaa
     4141 acgccaattg gttgaaactc gccaaatcac taagcatgtg gcacaaattt tggatagtcg
     4201 catgaatact aaatacgatg aaaatgataa acttattcga gaggttaaag tgattacctt
     4261 aaaatctaaa ttagtttctg acttccgaaa agatttccaa ttctataaag tacgtgagat
     4321 taacaattac catcatgccc atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat
     4381 taagaaatat ccaaaacttg aatcggagtt tgtctatggt gattataaag tttatgatgt
     4441 tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt
     4501 ttactctaat atcatgaact tcttcaaaac agaaattaca cttgcaaatg gagagattcg
     4561 caaacgccct ctaatcgaaa ctaatgggga aactggagaa attgtctggg ataaagggcg
     4621 agattttgcc acagtgcgca aagtattgtc catgccccaa gtcaatattg tcaagaaaac
     4681 agaagtacag acaggcggat tctccaagga gtcaatttta ccaaaaagaa attcggacaa
     4741 gcttattgct cgtaaaaaag actgggatcc aaaaaaatat ggtggttttg atagtccaac
     4801 ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa
     4861 atccgttaaa gagttactag ggatcacaat tatggaaaga agttcctttg aaaaaaatcc
     4921 gattgacttt ttagaagcta aaggatataa ggaagttaaa aaagacttaa tcattaaact
     4981 acctaaatat agtctttttg agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg
     5041 agaattacaa aaaggaaatg agctggctct gccaagcaaa tatgtgaatt ttttatattt
     5101 agctagtcat tatgaaaagt tgaagggtag tccagaagat aacgaacaaa aacaattgtt
     5161 tgtggagcag cataagcatt atttagatga gattattgag caaatcagtg aattttctaa
     5221 gcgtgttatt ttagcagatg ccaatttaga taaagttctt agtgcatata acaaacatag
     5281 agacaaacca atacgtgaac aagcagaaaa tattattcat ttatttacgt tgacgaatct
     5341 tggagctccc gctgctttta aatattttga tacaacaatt gatcgtaaac gatatacgtc
     5401 tacaaaagaa gttttagatg ccactcttat ccatcaatcc atcactggtc tttatgaaac
     5461 acgcattgat ttgagtcagc taggaggtga ccaccaccac caccaccact gagatccggc
     5521 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     5581 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     5641 atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
     5701 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
     5761 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagctagct tatcgatgat
     5821 aagctgtcaa acatgagaat taattcttga agacgaaagg gcctcgtgat acgcctattt
     5881 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5941 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6001 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6061 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6121 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6181 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6241 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6301 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6361 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6421 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6481 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6541 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6601 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6661 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6721 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6781 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6841 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6901 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6961 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7021 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7081 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7141 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7201 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     7261 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7321 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7381 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7441 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7501 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7561 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7621 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7681 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7741 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7801 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     7861 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
     7921 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     7981 ctgggtcatg gctgcgcccc gacacccacc aacacccgct gacgcgccct gacgggcttg
     8041 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     8101 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     8161 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     8221 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     8281 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     8341 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     8401 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     8461 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     8521 tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact
     8581 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     8641 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     8701 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     8761 ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
     8821 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
     8881 aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg
     8941 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct
     9001 acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc
     9061 agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc
     9121 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     9181 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     9241 aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     9301 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     9361 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     9421 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     9481 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     9541 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
     9601 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9661 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     9721 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     9781 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     9841 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
     9901 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     9961 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
    10021 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
    10081 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
    10141 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
    10201 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
    10261 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
    10321 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
    10381 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
    10441 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
    10501 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
    10561 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
    10621 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
    10681 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
    10741 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
    10801 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
    10861 g
//
