LOCUS       sequence-273578-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged Golgi apparatus marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-EGFP-Membrin-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..1188
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          237..1179
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1136..1156
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1243..1252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1249..1251,1252..1254,1255..1965)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1249..1251,1252..1254,1255..1965)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1294..1315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1555..1574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1902..1923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2624..3194
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2788..2805)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3015..3034
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3227..3826
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3227..3246)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3723..3743
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3840..4428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3893..3913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4311..4322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4635..5268
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5396..5412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5396..5412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5409..5431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5420..5436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5444..5450,5451..5468,5469..5474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5489..5510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5627..5644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5798..6386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5878..5897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6557..7348,7349..7417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7180..7199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7418..7522)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7570..7704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7607..7626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7661..7680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7872..8505
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8552..8677
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9174..9407
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9592..9636
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9899..10014
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
       61 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      121 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      181 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      241 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      301 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      361 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      421 gaggcctggc ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      481 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      541 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      601 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      661 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      721 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
      781 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
      841 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
      901 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
      961 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1021 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1081 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1141 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg atctatttcc
     1201 ggtgaattcc tcgagactag ttctagagcg gccgcaccgg tcgccaccat ggtgagcaag
     1261 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1321 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1381 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1441 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1501 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1561 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1621 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1681 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1741 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1801 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1861 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1921 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtactc agatctcgac
     1981 atggatcccc tgttccagca aacgcacaag caggtccacg agatccagtc ttgcatggga
     2041 cgcctggaga cggcagacaa gcagtctgtg cacatagtag aaaacgaaat ccaagcaagc
     2101 atagaccaga tattcagccg tctagaacgt ctggagattt tgtccagcaa ggagccccct
     2161 aacaaaaggc aaaatgccag acttcgggtt gaccagttaa agtatgatgt ccagcacctg
     2221 cagactgcgc tcagaaactt ccagcatcgg cgccatgcaa gggagcagca ggagagacag
     2281 cgagaagagc ttctgtctcg aaccttcacc actaacgact ctgacaccac cataccaatg
     2341 gacgaatcac tgcagtttaa ctcctccctc cagaaagttc acaacggcat ggatgacctc
     2401 attttagatg ggcacaatat tttagatgga ctgaggaccc agagactgac cttgaagggg
     2461 actcagaaga agatccttga cattgccaac atgctgggct tgtccaacac agtgatgcgg
     2521 ctcatcgaga agcgggcttt ccaggacaag tactttatga taggtgggat gctgctgacc
     2581 tgtgtggtca tgttcctcgt ggtgcagtac ctgacatgag aattccgccc cccccccccc
     2641 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     2701 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     2761 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     2821 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     2881 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     2941 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     3001 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     3061 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     3121 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     3181 aaacacgatg ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa
     3241 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     3301 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     3361 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     3421 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     3481 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     3541 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     3601 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     3661 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     3721 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     3781 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctagacgc gtctggaaca
     3841 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3901 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3961 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4021 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4081 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4141 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4201 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4261 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4321 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4381 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     4441 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     4501 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4561 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4621 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     4681 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     4741 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     4801 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     4861 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     4921 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     4981 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     5041 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     5101 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5161 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5221 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5281 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     5341 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5461 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5521 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5581 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5641 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5701 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7141 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7561 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7681 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     7741 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     7801 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     7861 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     7921 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7981 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8041 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     8101 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     8161 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     8221 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     8281 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     8341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8521 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8581 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8641 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8701 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8761 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8821 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8881 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8941 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9001 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     9061 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9121 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9181 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9241 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9301 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9361 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9421 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9481 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9541 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9601 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9661 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9721 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9781 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     9841 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgcctt
     9901 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     9961 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    10021 ttattacagg gacagcagag atccagttta tcgatgagta attcatacaa aaggactcgc
    10081 ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag aacccagaga
    10141 tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg gagaagagca
    10201 tg
//
