LOCUS       sequence-273589-        3394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial Himar1 mini-transposon donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Chen SP, Wang HH
  TITLE     An Engineered Cas-Transposon System for Programmable and 
            Site-Directed DNA Transpositions.
  JOURNAL   CRISPR J. 2019 Dec;2(6):376-394. doi: 10.1089/crispr.2019.0030. Epub
            2019 Nov 19.
  PUBMED    31742433
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2019.0030"; journalName: "CRISPR J"; date: 
            "2019-12"; volume: "2"; issue: "6"; pages: "376-394"
COMMENT     Sequence Label: pHimar6
FEATURES             Location/Qualifiers
     source          1..3394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      51..439
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            554..663
                     /note="incP origin of transfer"
     CDS             complement(984..1793)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1707..1726
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1794..1898)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1966..1984
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2393..3052
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2459..2478)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3109..3195
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3287..3314
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctctagagta gtagtccatg ggtatggaca gcggtggagg ggaattccca tgtcagccgt
       61 taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt
      121 acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat
      181 tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta
      241 attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc
      301 atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg
      361 ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg
      421 aactgctgat cttcagatcc tctacgccgg acgcatcgtg gccggatcaa ttccgtgata
      481 ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt
      541 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
      601 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
      661 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
      721 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
      781 ggttatgcag cggaaaacgg aattgatccc cctcgaattg acgcgtaacc agcaatagac
      841 ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa
      901 tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg
      961 caccaataac tgccttaaaa aaattagaaa aactcatcga gcatcaaatg aaactgcaat
     1021 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     1081 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     1141 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     1201 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct
     1261 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     1321 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     1381 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     1441 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt tccggggatc
     1501 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     1561 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     1621 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caagcgatag
     1681 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     1741 tccatgttgg aatttaatcg cggcctcgac gtttcccgtt gaatatggct catactcttc
     1801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1921 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     1981 aggccctttc gtcacgcgtc ctcggtacct aacaggttgg atgataagtc cccggtcttc
     2041 gtatgccgtc ttctgcttgg cgcgccctcg agcaattgcc gaccgaattt ttatgtcgta
     2101 aagaggggct ttgcaggggg tggactcaga aagatgagaa tagatgacta ttgtagttga
     2161 aacacataga aagttgctga tatacagacc gatacgcata tcgggatgaa ccatgagtac
     2221 gttcttttct caaaaaacat aaatattcga aaagagatgc aataaattaa ggagaggtta
     2281 tactctagag tagtagatta ttttaggaat ttagatgttt tgtatgaaat agatgcttcg
     2341 tatggaatta atgaaatttt tagtcaggta aaaaaggtaa taggagaata ttatggagaa
     2401 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     2461 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     2521 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     2581 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
     2641 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     2701 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     2761 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     2821 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     2881 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     2941 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     3001 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaaaacaata
     3061 ggccacatgc aactgtctag aatgcgagag tagggaactg ccaggcatca aataaaacga
     3121 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     3181 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     3241 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     3301 acggatggcc tttttgcgtt tctacctgca gggcgcgcca agcagaagac ggcatacgaa
     3361 gaccggggac ttatcatcca acctgttagg atcc
//
