LOCUS       sequence-27364-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Snider L, Asawachaicharn A, Tyler AE, Geng LN, Petek LM, Maves L, 
            Miller DG, Lemmers RJ, Winokur ST, Tawil R, van der Maarel SM, 
            Filippova GN, Tapscott SJ
  TITLE     RNA transcripts, miRNA-sized fragments and proteins produced from 
            D4Z4 units: new candidates for the pathophysiology of 
            facioscapulohumeral dystrophy.
  JOURNAL   Hum Mol Genet. 2009 Jul 1;18(13):2414-30. doi: 10.1093/hmg/ddp180. 
            Epub 2009 Apr 9.
  PUBMED    19359275
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddp180"; journalName: "Hum Mol Genet"; date: 
            "2009-07-1- 1"; volume: "18"; issue: "13"; pages: "2414-30"
COMMENT     Sequence Label: pCS2-mqgDUX4
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    266..400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(290..309)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     344..363
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(513..533)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(513..531)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(552..568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(552..568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(565..587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    576..592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(600..606,607..624,625..630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    645..666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gatccatgca aggcatcccg gcgccctccc aggcgctcca ggagccggcg ccctggtctg
       61 cactcccctg cggcctgctg ctggatgagc tcctggcgag cccggagttt ctgcagcagg
      121 cgcaacctct cctagaaacg gaggccccgg gggagctgga ggcctcggaa gaggccgcct
      181 cgctggaagc acccctcagc gaggaagaat accgggctct gctggaggag ctttagtcta
      241 gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg
      301 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      361 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      421 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc
      481 aatgcattgg gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
      541 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      601 aacatacgag ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      661 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      721 cattaatgaa tcggccaacg cgc
//
