LOCUS       sequence-273645-        9886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentivirus vector to integrate a surface membrane anchored human 
            IL3 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Liu T, Jia P, Ma H, Reed SA, Luo X, Larman HB, Schultz PG
  TITLE     Construction and Screening of a Lentiviral Secretome Library.
  JOURNAL   Cell Chem Biol. 2017 Jun 22;24(6):767-771.e3. doi: 
            10.1016/j.chembiol.2017.05.017. Epub 2017 Jun 9.
  PUBMED    28602759
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2017.05.017"; journalName: "Cell Chem Biol"; 
            date: "2017-06-22- 22"; volume: "24"; issue: "6"; pages: 
            "767-771.e3"
COMMENT     Sequence Label: pLV-EF1alpha-IL3-218-PDGFR
FEATURES             Location/Qualifiers
     source          1..9886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2561..3723
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2792..3714
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3671..3691
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3741..3765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4222..4246)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4302..4322
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4332..4478
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    4532..5120
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4585..4605)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5003..5014)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5123..5139)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5124..5140)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5645..5825
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5851..5868)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5857..6081
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6127..6555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6214..6233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6424..6445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6564..6584)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6569..6898
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6749..6884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6811..6830
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6946..6993
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7012..7386
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7516..7637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7553..7572)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7607..7626
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7686..7702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7686..7702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7699..7721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7710..7726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7734..7740,7741..7758,7759..7764))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7779..7800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7917..7934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8088..8676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8168..8187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8847..9638,9639..9707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9470..9489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9708..9812)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaacgtga ggctccggtg cccgtcagtg
     2581 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2641 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2701 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2761 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2821 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctccagta
     2881 cgtgattctt gatcccgagc tggagccagg ggcgggcctt gcgctttagg agccccttcg
     2941 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     3001 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     3061 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca ggatctgcac
     3121 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     3181 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     3241 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     3301 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     3361 gctccagggg gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     3421 acacaaagga aaggggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     3481 cgggcgccgt ccaggcacct cgattagttc tggagctttt ggagtacgtc gtctttaggt
     3541 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     3601 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     3661 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     3721 tgaacacgtg gtcgtctaga acaagtttgt acaaaaaagt tggcatgagc cgcctgcccg
     3781 tcctgctcct gctccaactc ctggtccgcc ccggactcca agctcccatg acccagacaa
     3841 cgcccttgaa gacaagctgg gttaactgct ctaacatgat cgatgaaatt ataacacact
     3901 taaagcagcc acctttgcct ttgctggact tcaacaacct caatggggaa gaccaagaca
     3961 ttctgatgga aaataacctt cgaaggccaa acctggaggc attcaacagg gctgtcaaga
     4021 gtttacagaa cgcatcagca attgagagca ttcttaaaaa tctcctgcca tgtctgcccc
     4081 tggccacggc cgcacccacg cgacatccaa tccatatcaa ggacggtgac tggaatgaat
     4141 tccggaggaa actgacgttc tatctgaaaa cccttgagaa tgcgcaggct caacagacga
     4201 ctttgagcct cgcgatcttt tgcccaactt tcttgtacaa agtggttggc tccaccagcg
     4261 gcagcggcaa gccaggctcc ggcgaaggca gcaccaaagg cgaaaacctg tattttcagg
     4321 gcgatctgaa tgctgtgggc caggacacgc aggaggtcat cgtggtgcca cactccttgc
     4381 cctttaaggt ggtggtgatc tcagccatcc tggccctggt ggtgctcacc atcatctccc
     4441 ttatcatcct catcatgctt tggcagaaga agccacgtat agggatccgt tgataaggat
     4501 ccgttaacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     4561 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4621 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4681 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4741 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4801 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4861 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4921 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4981 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5041 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5101 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     5161 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     5221 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     5281 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5341 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     5401 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     5461 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     5521 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     5581 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     5641 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5701 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5761 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5821 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     5881 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5941 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6001 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6061 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6121 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6181 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6241 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6301 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6361 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6421 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6481 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6541 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6601 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6661 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6721 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6781 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     6841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6901 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     6961 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     7021 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     7081 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     7141 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     7201 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     7261 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     7321 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     7381 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7441 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7501 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7561 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7621 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7681 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7741 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7801 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7861 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7921 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7981 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8041 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8101 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8161 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8221 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8281 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8341 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8401 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8461 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8521 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8701 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8761 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8821 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8881 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8941 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9001 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9061 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9121 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9181 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9241 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9301 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9361 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9421 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9481 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9541 tcaaggatct taccgctgtt gagatccagt tctatgtaac ccactcgtgc acccaactga
     9601 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9661 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9721 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9781 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9841 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcag
//
