LOCUS       sequence-273650-        9865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentivirus vector to integrate a surface membrane anchored human 
            CSF2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Liu T, Jia P, Ma H, Reed SA, Luo X, Larman HB, Schultz PG
  TITLE     Construction and Screening of a Lentiviral Secretome Library.
  JOURNAL   Cell Chem Biol. 2017 Jun 22;24(6):767-771.e3. doi: 
            10.1016/j.chembiol.2017.05.017. Epub 2017 Jun 9.
  PUBMED    28602759
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2017.05.017"; journalName: "Cell Chem Biol"; 
            date: "2017-06-22- 22"; volume: "24"; issue: "6"; pages: 
            "767-771.e3"
COMMENT     Sequence Label: pLV-EF1alpha-CSF2-218-PDGFR
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2561..3723
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2792..3714
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3671..3691
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3741..3765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4201..4225)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4281..4301
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4311..4457
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    4511..5099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4564..4584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4982..4993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5102..5118)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5103..5119)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5624..5804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5830..5847)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5836..6060
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6106..6534
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6193..6212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6403..6424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6543..6563)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6548..6877
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6728..6863
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6790..6809
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6925..6972
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6991..7365
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7495..7616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7532..7551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7586..7605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7665..7681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7665..7681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7678..7700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7689..7705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7713..7719,7720..7737,7738..7743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7758..7779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7896..7913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8067..8655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8147..8166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8826..9617,9618..9686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9449..9468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9687..9791)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaacgtga ggctccggtg cccgtcagtg
     2581 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2641 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2701 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2761 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2821 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctccagta
     2881 cgtgattctt gatcccgagc tggagccagg ggcgggcctt gcgctttagg agccccttcg
     2941 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     3001 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     3061 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca ggatctgcac
     3121 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     3181 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     3241 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     3301 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     3361 gctccagggg gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     3421 acacaaagga aaggggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     3481 cgggcgccgt ccaggcacct cgattagttc tggagctttt ggagtacgtc gtctttaggt
     3541 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     3601 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     3661 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     3721 tgaacacgtg gtcgtctaga acaagtttgt acaaaaaagt tggcaccatg tggctgcaga
     3781 gcctgctgct cttgggcact gtggcctgca gcatctctgc acccgcccgc tcgcccagcc
     3841 ccagcacgca gccctgggag catgtgaatg ccatccagga ggcccggcgt ctcctgaacc
     3901 tgagtagaga cactgctgct gagatgaatg aaacagtaga agtcatctca gaaatgtttg
     3961 acctccagga gccgacctgc ctacagaccc gcctggagct gtacaagcag ggcctgcggg
     4021 gcagcctcac caagctcaag ggccccttga ccatgatggc cagccactac aagcagcact
     4081 gccctccaac cccggaaact tcctgtgcaa cccagattat cacctttgaa agtttcaaag
     4141 agaacctgaa ggactttctg cttgtcatcc cctttgactg ctgggagcca gtccaggagt
     4201 tgccaacttt cttgtacaaa gtggttggct ccaccagcgg cagcggcaag ccaggctccg
     4261 gcgaaggcag caccaaaggc gaaaacctgt attttcaggg cgatctgaat gctgtgggcc
     4321 aggacacgca ggaggtcatc gtggtgccac actccttgcc ctttaaggtg gtggtgatct
     4381 cagccatcct ggccctggtg gtgctcacca tcatctccct tatcatcctc atcatgcttt
     4441 ggcagaagaa gccacgtata gggatccgtt gataaggatc cgttaacaat tcgatatcaa
     4501 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4561 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4621 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4681 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4741 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4801 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4861 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4921 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4981 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5041 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5101 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     5161 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     5221 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5281 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     5341 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     5401 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     5461 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     5521 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     5581 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     5641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     5821 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     5881 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     5941 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6001 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6061 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6121 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6181 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6241 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6301 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6361 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6421 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6481 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6541 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6601 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6661 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6721 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6781 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     6841 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     6901 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     6961 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     7021 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7081 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7141 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     7201 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     7261 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     7321 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     7381 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7441 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7501 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7561 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7621 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7681 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7741 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7801 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7861 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7921 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7981 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8041 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8101 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8161 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8221 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8281 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8341 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8401 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8461 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8521 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8581 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8641 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8701 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8761 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8821 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8881 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8941 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9001 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9061 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9121 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9181 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9241 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9301 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9361 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9421 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9481 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9541 agatccagtt ctatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9601 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9661 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9721 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9781 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     9841 ccgatcccct atggtgcact ctcag
//
