LOCUS       sequence-273653-       11980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible p53DD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11980)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 11980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pSLIK Zeo p53DD
FEATURES             Location/Qualifiers
     source          1..11980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2377..2401
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2433..2747
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2441..2459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2477..2495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2512..2530
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2548..2566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2584..2602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2655..2673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3098..3122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3172..4383
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4220..4239
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4442..5059,5060..5065,5066..5101,5102..5104,5105..5140,
                     5141..5143,5144..5179,5180..5188)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5333..5795
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5389..5406)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5616..5635
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        5817..5864
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5883..6257
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    6431..7019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6484..6504)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6902..6913)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7022..7038)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7023..7039)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7548..7728
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7754..7771)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7760..7984
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8030..8458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8117..8136)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8327..8348
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8467..8487)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8472..8801
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8652..8787
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8714..8733
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8849..8896
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8915..9289
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9419..9540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9456..9475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9510..9529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9589..9605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9589..9605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9602..9624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9613..9629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9637..9643,9644..9661,9662..9667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9682..9703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9820..9837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9991..10579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10071..10090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10750..11541,11542..11610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11373..11392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11611..11715)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11790..11809)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc
     2401 tttaaaggaa ccaattcagt cgactggatc ccgagtttac tccctatcag tgatagagaa
     2461 cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca
     2521 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     2581 tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg
     2641 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgccact agtatgactg
     2761 ccatggagga gtcacagtcg gatatcagcc tcgagagagc gctgcccacc tgcacaagcg
     2821 cctctccccc gcaaaagaaa aaaccacttg atggagagta tttcaccctc aagatccgcg
     2881 ggcgtaaacg cttcgagatg ttccgggagc tgaatgaggc cttagagtta aaggatgccc
     2941 atgctacaga ggagtctgga gacagcaggg ctcactccag ctacctgaag accaagaagg
     3001 gccagtctac ttcccgccat aaaaaaacaa tggtcaagaa agtggggcct gactcagact
     3061 gacaattgga agactaatgc gttttcgaga tatctagacc cagctttctt gtacaaagtg
     3121 gttgataaca tctcgagcca taaagatggt taattaaccc acccaagatc tggcctccgc
     3181 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
     3241 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
     3301 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
     3361 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
     3421 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
     3481 aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
     3541 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
     3601 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
     3661 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
     3721 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
     3781 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     3841 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     3901 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     3961 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     4021 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     4081 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     4141 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     4201 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     4261 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     4321 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     4381 gacgaagctt ggtaccgagc tcggatctcc accccgtacc ggtcctgcag tcgaattcac
     4441 catgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt
     4501 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     4561 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     4621 ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg
     4681 gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca
     4741 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     4801 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     4861 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     4921 acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca
     4981 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     5041 gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct
     5101 cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga
     5161 ttttgacctt gacatgctcc ccgggtaact aagtaaggat ccgcggccgc actagaggaa
     5221 ttccgcccct ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     5281 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     5341 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     5401 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     5461 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     5521 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5581 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     5641 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5701 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     5761 gggacgtggt tttcctttga aaaacacgat gataagctta ccggtacgcg tgatgtgttg
     5821 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     5881 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     5941 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     6001 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     6061 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     6121 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     6181 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     6241 ccgaggagca ggactgacac atctgtacaa gtaaagcggc cgcgactcta gatcataatc
     6301 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     6361 aacctgaaac ataaaatgaa tgcaattgtt gttgtttagt ccctcccaat tcgatatcaa
     6421 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6481 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6541 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6601 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6661 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6721 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6781 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6841 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6901 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6961 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7021 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     7081 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7141 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7201 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7261 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     7321 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     7381 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     7441 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     7501 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     7561 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7621 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7681 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     7741 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     7801 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     7861 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     7921 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     7981 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     8041 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8101 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8161 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8221 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8281 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8341 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8401 ccgatttcgg cctattgttt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     8461 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8521 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     8581 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     8641 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8701 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     8761 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     8821 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     8881 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     8941 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     9001 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     9061 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     9121 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     9181 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     9241 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     9301 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9361 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9421 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9481 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9541 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9601 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9661 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9721 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9781 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9841 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9901 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9961 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10021 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10081 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10141 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10201 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10261 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10321 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10381 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10441 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10501 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10561 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10621 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10681 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10741 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10801 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10861 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10921 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10981 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11041 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11101 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11161 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11221 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11281 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11341 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11401 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11461 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11521 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11581 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11641 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11701 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    11761 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    11821 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
    11881 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
    11941 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
