LOCUS       sequence-273655-        3205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Prochlorococcus phage P-SSM2 phage Fd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Campbell IJ, Olmos JL Jr, Xu W, Kahanda D, Atkinson JT, Sparks ON, 
            Miller MD, Phillips GN Jr, Bennett GN, Silberg JJ
  TITLE     Prochlorococcus phage ferredoxin: Structural characterization and 
            electron transfer to cyanobacterial sulfite reductases.
  JOURNAL   J Biol Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501.
  PUBMED    32434930
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2020 May 19. pii: RA120.013501. doi: 
            10.1074/jbc.RA120.013501."
COMMENT     Sequence Label: pFd022.R2
FEATURES             Location/Qualifiers
     source          1..3205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..19,20..25,26..42,43..48,49..74)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    26..44
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      430..501
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      517..544
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        596..630
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             665..1291
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(741..760)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      1297..1383
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1548..2136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1628..1647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2224..2318)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2339..2998)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2913..2932
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3167..3185
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc
       61 aggacgcact gaccgtataa acggggaata tagaaaagag ggttctatgg cgtatagcat
      121 taccctgcgc tcgccggatg gcgctgaaga agtggtacag tgcgaggaag atcagtatat
      181 tttagaagcg gcggaggatg ctgggctgga tatgccatca agttgccgtg cgggcgcttg
      241 ctccgcttgt ctcggcaaag tgttagaggg gtcagttaac aatgaggaac agtctttttt
      301 agacgacgat cagctggaag aaggatggtc actgctttgc gtggcaatgc cacagtcgga
      361 ctgtgttatc ctgacggagc aagaagacaa cctggattaa gaattctgat aataatctag
      421 accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      481 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      541 tgcgtttata ggtacccgtg gatcctctag agtcgacctg caggcatgca agctttttac
      601 ggctagctca gtcctaggta tagtgctagc ccagccagag aaacactctt taacaggggg
      661 tagtatgatg tctcgtttag ataaaagtaa agtgattaac agcgcattag agctgcttaa
      721 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
      781 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
      841 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
      901 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
      961 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
     1021 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgcagtggg
     1081 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
     1141 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
     1201 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
     1261 aaaacaactt aaatgtgaaa gtgggtctta aggcatcaaa taaaacgaaa ggctcagtcg
     1321 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     1381 aatccgccgc cctagaccta gggcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1441 gcggtaatac ggttatccac agaatcaggg gataacgcat gcaagaacat gtgagcaaaa
     1501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1981 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gactagtgct
     2221 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     2281 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     2341 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     2401 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2461 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2521 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2581 caataaaccc tttagggaaa taggccaggt tttcaccata acacgccaca tcttgcgaat
     2641 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     2701 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     2761 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     2821 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2881 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2941 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     3001 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgaccggt agtgatctta
     3061 tttcattatg gtgaaagttg gaacctctta cgggccggcc aacgtctcat tttcgccaga
     3121 tatcgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3181 gaggcccttt cgtcttcacc tcgag
//
